Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_192 and Pf1N1B4_193 are separated by 205 nucleotides Pf1N1B4_193 and Pf1N1B4_194 are separated by 101 nucleotides Pf1N1B4_194 and Pf1N1B4_195 are separated by 223 nucleotides
Pf1N1B4_192 - Cysteine desulfurase (EC 2.8.1.7), at 241,196 to 242,362
_192
Pf1N1B4_193 - Transporter, LysE family, at 242,568 to 243,182
_193
Pf1N1B4_194 - ABC-type proline/glycine betaine transport systems, ATPase components, at 243,284 to 243,553
_194
Pf1N1B4_195 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 243,777 to 244,235
_195
Position (kb)
242
243
244 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 241.710 kb on + strand, within Pf1N1B4_192 at 241.710 kb on + strand, within Pf1N1B4_192 at 241.710 kb on + strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.711 kb on - strand, within Pf1N1B4_192 at 241.964 kb on - strand, within Pf1N1B4_192 at 241.964 kb on - strand, within Pf1N1B4_192 at 241.992 kb on + strand at 241.992 kb on + strand, within Pf1N1B4_192 at 241.992 kb on + strand at 241.993 kb on - strand at 241.993 kb on - strand, within Pf1N1B4_192 at 241.993 kb on - strand, within Pf1N1B4_192 at 241.993 kb on - strand at 241.993 kb on - strand, within Pf1N1B4_192 at 241.993 kb on - strand, within Pf1N1B4_192 at 242.344 kb on - strand at 242.344 kb on - strand at 242.363 kb on - strand at 242.462 kb on + strand at 242.462 kb on + strand at 242.463 kb on - strand at 242.501 kb on - strand at 242.510 kb on + strand at 242.534 kb on + strand at 242.534 kb on + strand at 242.761 kb on + strand, within Pf1N1B4_193 at 242.779 kb on + strand, within Pf1N1B4_193 at 242.851 kb on + strand, within Pf1N1B4_193 at 242.851 kb on + strand, within Pf1N1B4_193 at 242.852 kb on - strand, within Pf1N1B4_193 at 242.852 kb on - strand, within Pf1N1B4_193 at 242.932 kb on + strand, within Pf1N1B4_193 at 242.932 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.962 kb on + strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.963 kb on - strand, within Pf1N1B4_193 at 242.974 kb on + strand, within Pf1N1B4_193 at 242.974 kb on + strand, within Pf1N1B4_193 at 242.974 kb on + strand, within Pf1N1B4_193 at 242.975 kb on - strand, within Pf1N1B4_193 at 243.211 kb on + strand at 243.212 kb on - strand at 243.272 kb on + strand at 243.308 kb on + strand at 243.309 kb on - strand at 243.396 kb on + strand, within Pf1N1B4_194 at 243.465 kb on + strand, within Pf1N1B4_194 at 243.466 kb on - strand, within Pf1N1B4_194 at 243.466 kb on - strand, within Pf1N1B4_194 at 243.617 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.713 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.715 kb on + strand at 243.719 kb on + strand at 243.719 kb on + strand at 243.784 kb on + strand at 243.804 kb on + strand at 243.822 kb on + strand at 243.826 kb on + strand, within Pf1N1B4_195 at 243.826 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.938 kb on + strand, within Pf1N1B4_195 at 243.939 kb on - strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.029 kb on + strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.030 kb on - strand, within Pf1N1B4_195 at 244.055 kb on + strand, within Pf1N1B4_195 at 244.111 kb on - strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.153 kb on + strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195 at 244.154 kb on - strand, within Pf1N1B4_195
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 241,710 + Pf1N1B4_192 0.44 -1.6 241,710 + Pf1N1B4_192 0.44 +0.3 241,710 + Pf1N1B4_192 0.44 +0.8 241,711 - Pf1N1B4_192 0.44 -0.1 241,711 - Pf1N1B4_192 0.44 +1.3 241,711 - Pf1N1B4_192 0.44 -0.4 241,711 - Pf1N1B4_192 0.44 +1.5 241,711 - Pf1N1B4_192 0.44 +0.1 241,964 - Pf1N1B4_192 0.66 -0.2 241,964 - Pf1N1B4_192 0.66 +0.6 241,992 + -0.8 241,992 + Pf1N1B4_192 0.68 -0.2 241,992 + +0.2 241,993 - -0.2 241,993 - Pf1N1B4_192 0.68 -0.1 241,993 - Pf1N1B4_192 0.68 +1.3 241,993 - -0.8 241,993 - Pf1N1B4_192 0.68 +0.9 241,993 - Pf1N1B4_192 0.68 +0.2 242,344 - -0.8 242,344 - -1.8 242,363 - -0.4 242,462 + +0.3 242,462 + -0.2 242,463 - +0.0 242,501 - -0.5 242,510 + +0.8 242,534 + -0.2 242,534 + +1.3 242,761 + Pf1N1B4_193 0.31 +2.2 242,779 + Pf1N1B4_193 0.34 +0.2 242,851 + Pf1N1B4_193 0.46 -1.6 242,851 + Pf1N1B4_193 0.46 -0.8 242,852 - Pf1N1B4_193 0.46 -0.2 242,852 - Pf1N1B4_193 0.46 -0.8 242,932 + Pf1N1B4_193 0.59 -0.2 242,932 + Pf1N1B4_193 0.59 -1.2 242,962 + Pf1N1B4_193 0.64 +0.8 242,962 + Pf1N1B4_193 0.64 -1.2 242,962 + Pf1N1B4_193 0.64 -0.5 242,962 + Pf1N1B4_193 0.64 -0.6 242,962 + Pf1N1B4_193 0.64 -1.6 242,963 - Pf1N1B4_193 0.64 +1.2 242,963 - Pf1N1B4_193 0.64 -0.6 242,963 - Pf1N1B4_193 0.64 -0.6 242,963 - Pf1N1B4_193 0.64 +1.1 242,974 + Pf1N1B4_193 0.66 -1.6 242,974 + Pf1N1B4_193 0.66 -1.6 242,974 + Pf1N1B4_193 0.66 -0.8 242,975 - Pf1N1B4_193 0.66 -0.2 243,211 + -0.1 243,212 - -1.0 243,272 + -0.2 243,308 + +1.3 243,309 - +0.2 243,396 + Pf1N1B4_194 0.41 +0.8 243,465 + Pf1N1B4_194 0.67 +0.0 243,466 - Pf1N1B4_194 0.67 +1.3 243,466 - Pf1N1B4_194 0.67 -1.0 243,617 + -0.6 243,713 + -0.9 243,713 + +0.0 243,713 + -2.6 243,713 + +0.8 243,713 + +0.2 243,713 + -0.0 243,713 + -0.4 243,715 + -0.2 243,715 + -0.6 243,715 + -0.6 243,715 + -0.7 243,715 + -1.2 243,719 + +0.0 243,719 + +0.3 243,784 + +1.6 243,804 + -1.1 243,822 + -0.5 243,826 + Pf1N1B4_195 0.11 -0.2 243,826 + Pf1N1B4_195 0.11 -1.8 243,938 + Pf1N1B4_195 0.35 +0.2 243,938 + Pf1N1B4_195 0.35 -1.6 243,939 - Pf1N1B4_195 0.35 +0.9 244,029 + Pf1N1B4_195 0.55 +0.8 244,029 + Pf1N1B4_195 0.55 +0.5 244,029 + Pf1N1B4_195 0.55 +0.9 244,029 + Pf1N1B4_195 0.55 +0.0 244,030 - Pf1N1B4_195 0.55 -0.6 244,030 - Pf1N1B4_195 0.55 +0.2 244,030 - Pf1N1B4_195 0.55 -0.1 244,030 - Pf1N1B4_195 0.55 -1.2 244,055 + Pf1N1B4_195 0.61 +0.2 244,111 - Pf1N1B4_195 0.73 -1.0 244,153 + Pf1N1B4_195 0.82 -1.5 244,153 + -0.8 244,153 + Pf1N1B4_195 0.82 +1.2 244,153 + +0.8 244,153 + Pf1N1B4_195 0.82 -0.7 244,153 + Pf1N1B4_195 0.82 -0.2 244,153 + Pf1N1B4_195 0.82 -0.2 244,154 - Pf1N1B4_195 0.82 -1.2 244,154 - Pf1N1B4_195 0.82 +0.3
Or see this region's nucleotide sequence