Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1719 and Pf1N1B4_1720 are separated by 466 nucleotides Pf1N1B4_1720 and Pf1N1B4_1721 are separated by 111 nucleotides Pf1N1B4_1721 and Pf1N1B4_1722 are separated by 111 nucleotides Pf1N1B4_1722 and Pf1N1B4_1723 are separated by 85 nucleotides
Pf1N1B4_1719 - Protein hipA, at 1,967,201 to 1,967,959
_1719
Pf1N1B4_1720 - Helix-turn-helix motif, at 1,968,426 to 1,968,674
_1720
Pf1N1B4_1721 - DNA-binding heavy metal response regulator, at 1,968,786 to 1,969,151
_1721
Pf1N1B4_1722 - transcriptional regulator, Crp/Fnr family, at 1,969,263 to 1,969,949
_1722
Pf1N1B4_1723 - FIG028593: membrane protein, at 1,970,035 to 1,970,553
_1723
Position (kb)
1968
1969
1970 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1967.853 kb on + strand, within Pf1N1B4_1719 at 1967.975 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.003 kb on + strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.004 kb on - strand at 1968.249 kb on - strand at 1968.249 kb on - strand at 1968.267 kb on + strand at 1968.267 kb on + strand at 1968.267 kb on + strand at 1968.268 kb on - strand at 1968.268 kb on - strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.330 kb on + strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.331 kb on - strand at 1968.335 kb on - strand at 1968.335 kb on - strand at 1968.335 kb on - strand at 1968.441 kb on - strand at 1968.441 kb on - strand at 1968.693 kb on - strand at 1968.715 kb on - strand at 1968.724 kb on - strand at 1968.724 kb on - strand at 1968.759 kb on + strand at 1968.760 kb on - strand at 1968.760 kb on - strand at 1968.760 kb on - strand at 1969.071 kb on + strand, within Pf1N1B4_1721 at 1969.072 kb on - strand, within Pf1N1B4_1721 at 1969.072 kb on - strand, within Pf1N1B4_1721 at 1969.079 kb on - strand, within Pf1N1B4_1721 at 1969.079 kb on - strand, within Pf1N1B4_1721 at 1969.079 kb on - strand, within Pf1N1B4_1721 at 1969.138 kb on - strand at 1969.182 kb on - strand at 1969.210 kb on + strand at 1969.305 kb on - strand at 1969.318 kb on + strand at 1969.319 kb on - strand at 1969.319 kb on - strand at 1969.323 kb on + strand at 1969.324 kb on - strand at 1969.324 kb on - strand at 1969.324 kb on - strand at 1969.580 kb on - strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.654 kb on + strand, within Pf1N1B4_1722 at 1969.655 kb on - strand, within Pf1N1B4_1722 at 1969.767 kb on - strand, within Pf1N1B4_1722 at 1969.767 kb on - strand, within Pf1N1B4_1722 at 1969.792 kb on + strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.793 kb on - strand, within Pf1N1B4_1722 at 1969.824 kb on + strand, within Pf1N1B4_1722 at 1969.825 kb on - strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.874 kb on + strand, within Pf1N1B4_1722 at 1969.875 kb on - strand, within Pf1N1B4_1722 at 1969.882 kb on - strand at 1969.893 kb on + strand at 1969.893 kb on + strand at 1969.894 kb on - strand at 1969.894 kb on - strand at 1970.019 kb on + strand at 1970.020 kb on - strand at 1970.020 kb on - strand at 1970.020 kb on - strand at 1970.030 kb on + strand at 1970.030 kb on + strand at 1970.031 kb on - strand at 1970.031 kb on - strand at 1970.094 kb on + strand, within Pf1N1B4_1723 at 1970.095 kb on - strand, within Pf1N1B4_1723 at 1970.129 kb on + strand, within Pf1N1B4_1723 at 1970.129 kb on + strand, within Pf1N1B4_1723 at 1970.130 kb on - strand, within Pf1N1B4_1723
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,967,853 + Pf1N1B4_1719 0.86 +0.4 1,967,975 + +0.4 1,968,003 + +0.4 1,968,003 + +0.1 1,968,003 + +0.4 1,968,003 + -0.6 1,968,003 + +0.7 1,968,003 + -0.3 1,968,003 + -0.7 1,968,003 + -0.0 1,968,003 + +1.4 1,968,004 - +0.1 1,968,004 - +1.4 1,968,004 - -0.2 1,968,004 - -1.5 1,968,004 - +0.2 1,968,004 - -0.9 1,968,004 - -0.2 1,968,004 - +0.3 1,968,004 - -0.5 1,968,004 - -0.6 1,968,004 - +0.0 1,968,004 - +2.2 1,968,004 - -0.9 1,968,249 - -0.2 1,968,249 - +0.7 1,968,267 + +0.4 1,968,267 + +0.6 1,968,267 + -1.2 1,968,268 - +0.8 1,968,268 - +0.6 1,968,330 + +2.2 1,968,330 + +0.4 1,968,330 + -1.1 1,968,330 + -0.9 1,968,330 + -1.5 1,968,331 - +0.7 1,968,331 - -0.2 1,968,331 - -0.6 1,968,331 - +0.4 1,968,331 - +0.7 1,968,335 - -0.5 1,968,335 - -1.6 1,968,335 - -1.9 1,968,441 - +1.4 1,968,441 - -0.1 1,968,693 - +0.4 1,968,715 - -0.3 1,968,724 - +0.3 1,968,724 - -0.5 1,968,759 + +1.4 1,968,760 - +0.6 1,968,760 - -0.3 1,968,760 - +0.4 1,969,071 + Pf1N1B4_1721 0.78 +0.4 1,969,072 - Pf1N1B4_1721 0.78 -0.6 1,969,072 - Pf1N1B4_1721 0.78 +0.1 1,969,079 - Pf1N1B4_1721 0.80 +0.4 1,969,079 - Pf1N1B4_1721 0.80 -0.6 1,969,079 - Pf1N1B4_1721 0.80 -1.2 1,969,138 - -0.4 1,969,182 - -0.3 1,969,210 + +0.4 1,969,305 - +0.4 1,969,318 + -0.5 1,969,319 - -2.2 1,969,319 - +0.4 1,969,323 + +1.4 1,969,324 - -1.5 1,969,324 - +0.7 1,969,324 - +0.2 1,969,580 - Pf1N1B4_1722 0.46 +0.7 1,969,654 + Pf1N1B4_1722 0.57 +0.9 1,969,654 + Pf1N1B4_1722 0.57 +0.2 1,969,654 + Pf1N1B4_1722 0.57 +0.3 1,969,654 + Pf1N1B4_1722 0.57 -1.0 1,969,654 + Pf1N1B4_1722 0.57 -1.4 1,969,655 - Pf1N1B4_1722 0.57 +0.4 1,969,767 - Pf1N1B4_1722 0.73 +0.4 1,969,767 - Pf1N1B4_1722 0.73 -0.4 1,969,792 + Pf1N1B4_1722 0.77 -0.0 1,969,793 - Pf1N1B4_1722 0.77 -0.4 1,969,793 - Pf1N1B4_1722 0.77 +1.8 1,969,793 - Pf1N1B4_1722 0.77 +0.7 1,969,793 - Pf1N1B4_1722 0.77 +0.3 1,969,824 + Pf1N1B4_1722 0.82 +0.4 1,969,825 - Pf1N1B4_1722 0.82 +0.4 1,969,874 + Pf1N1B4_1722 0.89 -0.2 1,969,874 + Pf1N1B4_1722 0.89 -1.4 1,969,874 + Pf1N1B4_1722 0.89 -0.4 1,969,875 - Pf1N1B4_1722 0.89 +0.3 1,969,882 - -1.1 1,969,893 + +0.2 1,969,893 + +0.3 1,969,894 - +0.8 1,969,894 - +0.4 1,970,019 + -0.1 1,970,020 - +0.8 1,970,020 - -1.1 1,970,020 - -0.9 1,970,030 + +0.2 1,970,030 + +1.2 1,970,031 - -0.9 1,970,031 - +0.8 1,970,094 + Pf1N1B4_1723 0.11 +1.6 1,970,095 - Pf1N1B4_1723 0.12 -0.4 1,970,129 + Pf1N1B4_1723 0.18 +0.5 1,970,129 + Pf1N1B4_1723 0.18 +0.6 1,970,130 - Pf1N1B4_1723 0.18 +0.8
Or see this region's nucleotide sequence