Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1577 and Pf1N1B4_1578 are separated by 181 nucleotides Pf1N1B4_1578 and Pf1N1B4_1579 are separated by 10 nucleotides Pf1N1B4_1579 and Pf1N1B4_1580 are separated by 17 nucleotides
Pf1N1B4_1577 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 1,816,227 to 1,817,183
_1577
Pf1N1B4_1578 - N-formylglutamate deformylase (EC 3.5.1.68) (from data) , at 1,817,365 to 1,818,165
_1578
Pf1N1B4_1579 - Imidazolonepropionase (EC 3.5.2.7) (from data) , at 1,818,176 to 1,819,381
_1579
Pf1N1B4_1580 - Histidine transport protein (permease), at 1,819,399 to 1,820,808
_1580
Position (kb)
1818
1819
1820 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1817.184 kb on - strand at 1817.208 kb on + strand at 1817.209 kb on - strand at 1817.251 kb on + strand at 1817.251 kb on + strand at 1817.251 kb on + strand at 1817.252 kb on - strand at 1817.252 kb on - strand at 1817.282 kb on + strand at 1817.283 kb on - strand at 1817.283 kb on - strand at 1817.324 kb on - strand at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.494 kb on + strand, within Pf1N1B4_1578 at 1817.589 kb on - strand, within Pf1N1B4_1578 at 1817.589 kb on - strand, within Pf1N1B4_1578 at 1817.795 kb on + strand, within Pf1N1B4_1578 at 1817.819 kb on + strand, within Pf1N1B4_1578 at 1817.895 kb on - strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.897 kb on - strand, within Pf1N1B4_1578 at 1817.897 kb on - strand, within Pf1N1B4_1578 at 1817.897 kb on - strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.920 kb on + strand, within Pf1N1B4_1578 at 1817.921 kb on - strand, within Pf1N1B4_1578 at 1817.921 kb on - strand, within Pf1N1B4_1578 at 1817.921 kb on - strand, within Pf1N1B4_1578 at 1817.969 kb on + strand, within Pf1N1B4_1578 at 1818.159 kb on + strand at 1818.238 kb on - strand at 1818.525 kb on + strand, within Pf1N1B4_1579 at 1818.526 kb on - strand, within Pf1N1B4_1579 at 1818.831 kb on + strand, within Pf1N1B4_1579 at 1818.831 kb on + strand, within Pf1N1B4_1579 at 1818.831 kb on + strand, within Pf1N1B4_1579 at 1818.832 kb on - strand, within Pf1N1B4_1579 at 1818.832 kb on - strand, within Pf1N1B4_1579 at 1818.832 kb on - strand, within Pf1N1B4_1579 at 1818.852 kb on + strand, within Pf1N1B4_1579 at 1818.853 kb on - strand, within Pf1N1B4_1579 at 1819.120 kb on - strand at 1819.178 kb on - strand, within Pf1N1B4_1579 at 1819.395 kb on + strand at 1819.539 kb on - strand at 1819.751 kb on + strand, within Pf1N1B4_1580 at 1819.752 kb on - strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.871 kb on + strand, within Pf1N1B4_1580 at 1819.872 kb on - strand, within Pf1N1B4_1580 at 1819.872 kb on - strand, within Pf1N1B4_1580 at 1819.872 kb on - strand, within Pf1N1B4_1580 at 1819.872 kb on - strand, within Pf1N1B4_1580 at 1819.872 kb on - strand, within Pf1N1B4_1580 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.146 kb on + strand, within Pf1N1B4_1580
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,817,184 - +0.1 1,817,208 + -1.1 1,817,209 - +1.4 1,817,251 + -0.6 1,817,251 + -0.3 1,817,251 + -0.2 1,817,252 - -1.1 1,817,252 - -0.4 1,817,282 + -1.1 1,817,283 - -0.6 1,817,283 - +0.1 1,817,324 - -0.0 1,817,480 + Pf1N1B4_1578 0.14 -1.8 1,817,480 + Pf1N1B4_1578 0.14 +0.8 1,817,480 + Pf1N1B4_1578 0.14 +1.1 1,817,480 + Pf1N1B4_1578 0.14 +0.8 1,817,480 + Pf1N1B4_1578 0.14 +0.4 1,817,480 + Pf1N1B4_1578 0.14 -0.0 1,817,480 + Pf1N1B4_1578 0.14 +2.0 1,817,480 + Pf1N1B4_1578 0.14 -1.1 1,817,481 - Pf1N1B4_1578 0.14 +1.4 1,817,481 - Pf1N1B4_1578 0.14 -0.6 1,817,481 - Pf1N1B4_1578 0.14 +0.6 1,817,481 - Pf1N1B4_1578 0.14 +0.6 1,817,481 - Pf1N1B4_1578 0.14 +0.4 1,817,481 - Pf1N1B4_1578 0.14 +1.8 1,817,494 + Pf1N1B4_1578 0.16 -1.1 1,817,589 - Pf1N1B4_1578 0.28 +0.4 1,817,589 - Pf1N1B4_1578 0.28 -0.9 1,817,795 + Pf1N1B4_1578 0.54 +1.0 1,817,819 + Pf1N1B4_1578 0.57 -0.6 1,817,895 - Pf1N1B4_1578 0.66 -0.0 1,817,896 + Pf1N1B4_1578 0.66 +2.0 1,817,896 + Pf1N1B4_1578 0.66 -0.9 1,817,896 + Pf1N1B4_1578 0.66 -1.4 1,817,896 + Pf1N1B4_1578 0.66 -0.7 1,817,897 - Pf1N1B4_1578 0.66 +0.8 1,817,897 - Pf1N1B4_1578 0.66 +1.4 1,817,897 - Pf1N1B4_1578 0.66 +0.9 1,817,918 + Pf1N1B4_1578 0.69 +0.3 1,817,918 + Pf1N1B4_1578 0.69 +0.3 1,817,918 + Pf1N1B4_1578 0.69 +1.6 1,817,918 + +0.8 1,817,918 + Pf1N1B4_1578 0.69 +1.6 1,817,918 + Pf1N1B4_1578 0.69 +0.2 1,817,918 + Pf1N1B4_1578 0.69 -0.2 1,817,919 - Pf1N1B4_1578 0.69 -0.0 1,817,919 - Pf1N1B4_1578 0.69 -0.0 1,817,919 - Pf1N1B4_1578 0.69 -0.2 1,817,919 - +1.4 1,817,919 - Pf1N1B4_1578 0.69 -0.9 1,817,919 - Pf1N1B4_1578 0.69 -1.4 1,817,919 - Pf1N1B4_1578 0.69 +0.1 1,817,919 - Pf1N1B4_1578 0.69 -1.6 1,817,919 - Pf1N1B4_1578 0.69 +0.8 1,817,920 + Pf1N1B4_1578 0.69 +0.5 1,817,921 - Pf1N1B4_1578 0.69 +1.4 1,817,921 - Pf1N1B4_1578 0.69 +0.2 1,817,921 - Pf1N1B4_1578 0.69 +0.4 1,817,969 + Pf1N1B4_1578 0.75 -0.2 1,818,159 + -0.1 1,818,238 - +1.4 1,818,525 + Pf1N1B4_1579 0.29 +1.7 1,818,526 - Pf1N1B4_1579 0.29 -1.9 1,818,831 + Pf1N1B4_1579 0.54 -0.4 1,818,831 + Pf1N1B4_1579 0.54 -1.9 1,818,831 + Pf1N1B4_1579 0.54 +1.2 1,818,832 - Pf1N1B4_1579 0.54 -1.6 1,818,832 - Pf1N1B4_1579 0.54 +0.7 1,818,832 - Pf1N1B4_1579 0.54 +0.4 1,818,852 + Pf1N1B4_1579 0.56 -2.1 1,818,853 - Pf1N1B4_1579 0.56 +0.1 1,819,120 - +0.4 1,819,178 - Pf1N1B4_1579 0.83 -1.4 1,819,395 + -0.1 1,819,539 - +0.4 1,819,751 + Pf1N1B4_1580 0.25 -0.9 1,819,752 - Pf1N1B4_1580 0.25 -1.6 1,819,871 + Pf1N1B4_1580 0.33 -0.1 1,819,871 + Pf1N1B4_1580 0.33 -1.2 1,819,871 + Pf1N1B4_1580 0.33 +0.7 1,819,871 + Pf1N1B4_1580 0.33 -0.8 1,819,871 + Pf1N1B4_1580 0.33 +0.4 1,819,871 + Pf1N1B4_1580 0.33 +1.8 1,819,871 + Pf1N1B4_1580 0.33 -0.3 1,819,871 + Pf1N1B4_1580 0.33 -0.2 1,819,871 + Pf1N1B4_1580 0.33 +0.9 1,819,871 + Pf1N1B4_1580 0.33 -0.4 1,819,872 - Pf1N1B4_1580 0.34 +0.5 1,819,872 - Pf1N1B4_1580 0.34 -0.9 1,819,872 - Pf1N1B4_1580 0.34 -0.0 1,819,872 - Pf1N1B4_1580 0.34 +1.4 1,819,872 - Pf1N1B4_1580 0.34 -0.9 1,820,060 + Pf1N1B4_1580 0.47 +1.6 1,820,060 + Pf1N1B4_1580 0.47 +0.4 1,820,060 + Pf1N1B4_1580 0.47 +0.4 1,820,061 - Pf1N1B4_1580 0.47 +1.4 1,820,061 - Pf1N1B4_1580 0.47 +1.2 1,820,061 - Pf1N1B4_1580 0.47 +0.3 1,820,061 - Pf1N1B4_1580 0.47 +2.7 1,820,061 - Pf1N1B4_1580 0.47 +0.7 1,820,073 + Pf1N1B4_1580 0.48 -0.6 1,820,073 + Pf1N1B4_1580 0.48 +0.6 1,820,073 + Pf1N1B4_1580 0.48 -0.6 1,820,073 + Pf1N1B4_1580 0.48 +0.5 1,820,073 + Pf1N1B4_1580 0.48 +0.4 1,820,146 + Pf1N1B4_1580 0.53 +1.4
Or see this region's nucleotide sequence