Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1528 and Pf1N1B4_1529 are separated by 37 nucleotides Pf1N1B4_1529 and Pf1N1B4_1530 are separated by 45 nucleotides
Pf1N1B4_1528 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 1,760,651 to 1,761,034
_1528
Pf1N1B4_1529 - hypothetical protein, at 1,761,072 to 1,761,326
_1529
Pf1N1B4_1530 - LysR family transcriptional regulator YnfL, at 1,761,372 to 1,762,274
_1530
Position (kb)
1760
1761
1762 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1759.896 kb on - strand at 1760.066 kb on - strand at 1760.236 kb on - strand at 1760.360 kb on - strand at 1760.364 kb on - strand at 1760.451 kb on + strand at 1760.603 kb on + strand at 1760.958 kb on + strand, within Pf1N1B4_1528 at 1760.958 kb on + strand, within Pf1N1B4_1528 at 1760.958 kb on + strand, within Pf1N1B4_1528 at 1760.958 kb on + strand, within Pf1N1B4_1528 at 1760.964 kb on + strand, within Pf1N1B4_1528 at 1760.976 kb on + strand, within Pf1N1B4_1528 at 1760.976 kb on + strand, within Pf1N1B4_1528 at 1760.977 kb on - strand, within Pf1N1B4_1528 at 1761.036 kb on + strand at 1761.081 kb on + strand at 1761.081 kb on + strand at 1761.081 kb on + strand at 1761.081 kb on + strand at 1761.081 kb on + strand at 1761.081 kb on + strand at 1761.081 kb on + strand at 1761.082 kb on - strand at 1761.089 kb on + strand at 1761.089 kb on + strand at 1761.325 kb on - strand at 1761.374 kb on - strand at 1761.401 kb on + strand at 1761.402 kb on - strand at 1761.499 kb on + strand, within Pf1N1B4_1530 at 1761.515 kb on + strand, within Pf1N1B4_1530 at 1761.529 kb on + strand, within Pf1N1B4_1530 at 1761.658 kb on + strand, within Pf1N1B4_1530 at 1761.659 kb on - strand, within Pf1N1B4_1530 at 1761.659 kb on - strand, within Pf1N1B4_1530 at 1761.674 kb on - strand, within Pf1N1B4_1530 at 1761.690 kb on + strand, within Pf1N1B4_1530 at 1761.690 kb on + strand, within Pf1N1B4_1530 at 1761.690 kb on + strand, within Pf1N1B4_1530 at 1761.691 kb on - strand, within Pf1N1B4_1530 at 1761.723 kb on + strand, within Pf1N1B4_1530 at 1761.723 kb on + strand, within Pf1N1B4_1530 at 1761.724 kb on - strand, within Pf1N1B4_1530
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,759,896 - -0.4 1,760,066 - -0.6 1,760,236 - +0.2 1,760,360 - -0.4 1,760,364 - +0.1 1,760,451 + +2.0 1,760,603 + -0.9 1,760,958 + Pf1N1B4_1528 0.80 -0.6 1,760,958 + Pf1N1B4_1528 0.80 -0.1 1,760,958 + Pf1N1B4_1528 0.80 -0.0 1,760,958 + Pf1N1B4_1528 0.80 -0.6 1,760,964 + Pf1N1B4_1528 0.82 +0.4 1,760,976 + Pf1N1B4_1528 0.85 -1.6 1,760,976 + Pf1N1B4_1528 0.85 -1.2 1,760,977 - Pf1N1B4_1528 0.85 +0.6 1,761,036 + +0.1 1,761,081 + +0.5 1,761,081 + +0.6 1,761,081 + +0.8 1,761,081 + +0.2 1,761,081 + +1.4 1,761,081 + -1.2 1,761,081 + -1.2 1,761,082 - -1.8 1,761,089 + +0.1 1,761,089 + -1.4 1,761,325 - -0.4 1,761,374 - -2.0 1,761,401 + -0.6 1,761,402 - +0.1 1,761,499 + Pf1N1B4_1530 0.14 -0.6 1,761,515 + Pf1N1B4_1530 0.16 -0.1 1,761,529 + Pf1N1B4_1530 0.17 -0.2 1,761,658 + Pf1N1B4_1530 0.32 -0.5 1,761,659 - Pf1N1B4_1530 0.32 -0.2 1,761,659 - Pf1N1B4_1530 0.32 +0.7 1,761,674 - Pf1N1B4_1530 0.33 -0.2 1,761,690 + Pf1N1B4_1530 0.35 +2.4 1,761,690 + Pf1N1B4_1530 0.35 -0.0 1,761,690 + Pf1N1B4_1530 0.35 -0.1 1,761,691 - Pf1N1B4_1530 0.35 +0.7 1,761,723 + Pf1N1B4_1530 0.39 +0.7 1,761,723 + Pf1N1B4_1530 0.39 -0.9 1,761,724 - Pf1N1B4_1530 0.39 +2.2
Or see this region's nucleotide sequence