Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_151 and Pf1N1B4_152 are separated by 274 nucleotides Pf1N1B4_152 and Pf1N1B4_153 are separated by 213 nucleotides
Pf1N1B4_151 - Thioesterase in siderophore biosynthesis gene cluster, at 193,128 to 193,868
_151
Pf1N1B4_152 - ABC transporter, permease protein, at 194,143 to 195,264
_152
Pf1N1B4_153 - Glucoamylase (EC 3.2.1.3), at 195,478 to 197,301
_153
Position (kb)
194
195
196 Strain fitness (log2 ratio)
-2
-1
0
1 at 193.190 kb on + strand at 193.813 kb on + strand at 193.813 kb on + strand at 193.875 kb on + strand at 193.894 kb on + strand at 193.895 kb on - strand at 193.925 kb on + strand at 193.959 kb on + strand at 193.960 kb on - strand at 194.091 kb on + strand at 194.091 kb on + strand at 194.091 kb on + strand at 194.092 kb on - strand at 194.112 kb on + strand at 194.552 kb on + strand, within Pf1N1B4_152 at 194.718 kb on - strand, within Pf1N1B4_152 at 194.718 kb on - strand, within Pf1N1B4_152 at 194.725 kb on + strand, within Pf1N1B4_152 at 194.725 kb on + strand, within Pf1N1B4_152 at 194.856 kb on + strand at 194.857 kb on - strand, within Pf1N1B4_152 at 194.902 kb on + strand, within Pf1N1B4_152 at 195.024 kb on + strand, within Pf1N1B4_152 at 195.025 kb on - strand, within Pf1N1B4_152 at 195.445 kb on - strand at 195.449 kb on - strand at 195.871 kb on + strand, within Pf1N1B4_153 at 195.916 kb on + strand, within Pf1N1B4_153 at 195.917 kb on - strand, within Pf1N1B4_153 at 195.969 kb on - strand, within Pf1N1B4_153
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 193,190 + +0.4 193,813 + -0.3 193,813 + -1.1 193,875 + +1.0 193,894 + -1.6 193,895 - -0.7 193,925 + +1.3 193,959 + +0.7 193,960 - +0.7 194,091 + -1.1 194,091 + -1.8 194,091 + -0.8 194,092 - +0.3 194,112 + +0.4 194,552 + Pf1N1B4_152 0.36 -0.1 194,718 - Pf1N1B4_152 0.51 -2.4 194,718 - Pf1N1B4_152 0.51 -0.7 194,725 + Pf1N1B4_152 0.52 -0.3 194,725 + Pf1N1B4_152 0.52 -1.2 194,856 + -1.6 194,857 - Pf1N1B4_152 0.64 +1.2 194,902 + Pf1N1B4_152 0.68 -0.6 195,024 + Pf1N1B4_152 0.79 -0.1 195,025 - Pf1N1B4_152 0.79 -2.3 195,445 - +0.6 195,449 - +0.1 195,871 + Pf1N1B4_153 0.22 +1.6 195,916 + Pf1N1B4_153 0.24 -0.8 195,917 - Pf1N1B4_153 0.24 -0.8 195,969 - Pf1N1B4_153 0.27 -0.3
Or see this region's nucleotide sequence