Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1402 and Pf1N1B4_1403 overlap by 4 nucleotides Pf1N1B4_1403 and Pf1N1B4_5183 are separated by 226 nucleotides Pf1N1B4_5183 and Pf1N1B4_1404 are separated by 337 nucleotides
Pf1N1B4_1402 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 1,605,577 to 1,606,338
_1402
Pf1N1B4_1403 - 3'-to-5' exoribonuclease RNase R, at 1,606,335 to 1,608,965
_1403
Pf1N1B4_5183 - tRNA-Leu-CAG, at 1,609,192 to 1,609,275
_5183
Pf1N1B4_1404 - Ferric iron ABC transporter, iron-binding protein, at 1,609,613 to 1,610,623
_1404
Position (kb)
1606
1607
1608
1609 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1605.433 kb on + strand at 1605.434 kb on - strand at 1605.434 kb on - strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.463 kb on + strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.464 kb on - strand at 1605.535 kb on + strand at 1605.535 kb on + strand at 1605.536 kb on - strand at 1605.536 kb on - strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.562 kb on + strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.563 kb on - strand at 1605.699 kb on - strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.706 kb on + strand, within Pf1N1B4_1402 at 1605.707 kb on - strand, within Pf1N1B4_1402 at 1605.707 kb on - strand, within Pf1N1B4_1402 at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.316 kb on + strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.317 kb on - strand at 1606.349 kb on + strand at 1606.350 kb on - strand at 1606.544 kb on + strand at 1606.837 kb on - strand, within Pf1N1B4_1403 at 1606.855 kb on + strand, within Pf1N1B4_1403 at 1606.856 kb on - strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.897 kb on + strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1606.898 kb on - strand, within Pf1N1B4_1403 at 1607.230 kb on + strand, within Pf1N1B4_1403 at 1607.231 kb on - strand, within Pf1N1B4_1403 at 1607.239 kb on + strand at 1607.240 kb on - strand, within Pf1N1B4_1403 at 1607.275 kb on + strand, within Pf1N1B4_1403 at 1607.276 kb on - strand, within Pf1N1B4_1403 at 1607.276 kb on - strand, within Pf1N1B4_1403 at 1607.410 kb on - strand, within Pf1N1B4_1403 at 1607.410 kb on - strand, within Pf1N1B4_1403 at 1607.680 kb on + strand, within Pf1N1B4_1403 at 1607.723 kb on - strand, within Pf1N1B4_1403 at 1607.787 kb on - strand, within Pf1N1B4_1403 at 1607.788 kb on + strand, within Pf1N1B4_1403 at 1607.788 kb on + strand, within Pf1N1B4_1403 at 1607.789 kb on - strand, within Pf1N1B4_1403 at 1607.789 kb on - strand, within Pf1N1B4_1403 at 1607.824 kb on + strand, within Pf1N1B4_1403 at 1607.966 kb on - strand, within Pf1N1B4_1403 at 1607.966 kb on - strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.049 kb on + strand, within Pf1N1B4_1403 at 1608.050 kb on - strand, within Pf1N1B4_1403 at 1608.050 kb on - strand, within Pf1N1B4_1403 at 1608.050 kb on - strand, within Pf1N1B4_1403 at 1608.262 kb on + strand, within Pf1N1B4_1403 at 1608.263 kb on - strand at 1608.263 kb on - strand, within Pf1N1B4_1403 at 1608.263 kb on - strand, within Pf1N1B4_1403 at 1608.263 kb on - strand at 1608.304 kb on + strand, within Pf1N1B4_1403 at 1608.304 kb on + strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.305 kb on - strand, within Pf1N1B4_1403 at 1608.487 kb on + strand, within Pf1N1B4_1403 at 1608.727 kb on + strand at 1608.727 kb on + strand at 1608.727 kb on + strand at 1608.728 kb on - strand at 1608.728 kb on - strand at 1608.745 kb on + strand at 1608.745 kb on + strand at 1608.745 kb on + strand at 1608.746 kb on - strand at 1608.986 kb on + strand at 1608.997 kb on + strand at 1609.050 kb on + strand at 1609.367 kb on + strand at 1609.367 kb on + strand at 1609.367 kb on + strand at 1609.368 kb on - strand at 1609.434 kb on - strand at 1609.434 kb on - strand at 1609.434 kb on - strand at 1609.516 kb on + strand at 1609.517 kb on - strand at 1609.575 kb on + strand at 1609.627 kb on + strand at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.718 kb on + strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.719 kb on - strand, within Pf1N1B4_1404 at 1609.868 kb on + strand, within Pf1N1B4_1404 at 1609.868 kb on + strand, within Pf1N1B4_1404 at 1609.868 kb on + strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.869 kb on - strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.961 kb on + strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404 at 1609.962 kb on - strand, within Pf1N1B4_1404
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,605,433 + +0.1 1,605,434 - +0.7 1,605,434 - +0.4 1,605,463 + +0.8 1,605,463 + -1.6 1,605,463 + -0.1 1,605,463 + -0.3 1,605,463 + +0.3 1,605,464 - +0.9 1,605,464 - -0.5 1,605,464 - +2.3 1,605,464 - -0.3 1,605,464 - -0.7 1,605,535 + +1.3 1,605,535 + +1.9 1,605,536 - -0.8 1,605,536 - -1.3 1,605,562 + +0.1 1,605,562 + +0.9 1,605,562 + -0.3 1,605,562 + +0.3 1,605,562 + -1.3 1,605,562 + +2.3 1,605,563 - -0.3 1,605,563 - -0.1 1,605,563 - -0.7 1,605,563 - -1.4 1,605,563 - -0.3 1,605,563 - -0.1 1,605,699 - Pf1N1B4_1402 0.16 +0.0 1,605,706 + Pf1N1B4_1402 0.17 +0.5 1,605,706 + Pf1N1B4_1402 0.17 +0.5 1,605,706 + Pf1N1B4_1402 0.17 -0.0 1,605,706 + Pf1N1B4_1402 0.17 -0.3 1,605,707 - Pf1N1B4_1402 0.17 -1.0 1,605,707 - Pf1N1B4_1402 0.17 -1.2 1,606,316 + +0.9 1,606,316 + +1.0 1,606,316 + -0.3 1,606,316 + +0.5 1,606,317 - +1.7 1,606,317 - -0.5 1,606,317 - -0.3 1,606,317 - -0.5 1,606,349 + -2.4 1,606,350 - -0.1 1,606,544 + +0.8 1,606,837 - Pf1N1B4_1403 0.19 -0.1 1,606,855 + Pf1N1B4_1403 0.20 -0.5 1,606,856 - Pf1N1B4_1403 0.20 +1.3 1,606,897 + Pf1N1B4_1403 0.21 +1.3 1,606,897 + Pf1N1B4_1403 0.21 +0.6 1,606,897 + Pf1N1B4_1403 0.21 -2.1 1,606,897 + Pf1N1B4_1403 0.21 -0.3 1,606,898 - Pf1N1B4_1403 0.21 +0.7 1,606,898 - Pf1N1B4_1403 0.21 -0.3 1,606,898 - Pf1N1B4_1403 0.21 -0.4 1,607,230 + Pf1N1B4_1403 0.34 -1.2 1,607,231 - Pf1N1B4_1403 0.34 -0.7 1,607,239 + +0.3 1,607,240 - Pf1N1B4_1403 0.34 -1.8 1,607,275 + Pf1N1B4_1403 0.36 +0.3 1,607,276 - Pf1N1B4_1403 0.36 +1.9 1,607,276 - Pf1N1B4_1403 0.36 -0.3 1,607,410 - Pf1N1B4_1403 0.41 +0.9 1,607,410 - Pf1N1B4_1403 0.41 -1.3 1,607,680 + Pf1N1B4_1403 0.51 -1.3 1,607,723 - Pf1N1B4_1403 0.53 -1.1 1,607,787 - Pf1N1B4_1403 0.55 -1.3 1,607,788 + Pf1N1B4_1403 0.55 +0.7 1,607,788 + Pf1N1B4_1403 0.55 -1.7 1,607,789 - Pf1N1B4_1403 0.55 +2.3 1,607,789 - Pf1N1B4_1403 0.55 -1.8 1,607,824 + Pf1N1B4_1403 0.57 -1.3 1,607,966 - Pf1N1B4_1403 0.62 -0.7 1,607,966 - Pf1N1B4_1403 0.62 -0.3 1,608,049 + Pf1N1B4_1403 0.65 -0.1 1,608,049 + Pf1N1B4_1403 0.65 -1.0 1,608,049 + Pf1N1B4_1403 0.65 -1.0 1,608,049 + Pf1N1B4_1403 0.65 -1.4 1,608,050 - Pf1N1B4_1403 0.65 -1.3 1,608,050 - Pf1N1B4_1403 0.65 +0.3 1,608,050 - Pf1N1B4_1403 0.65 +0.6 1,608,262 + Pf1N1B4_1403 0.73 +0.9 1,608,263 - +0.3 1,608,263 - Pf1N1B4_1403 0.73 -1.4 1,608,263 - Pf1N1B4_1403 0.73 -0.7 1,608,263 - -0.3 1,608,304 + Pf1N1B4_1403 0.75 +0.3 1,608,304 + Pf1N1B4_1403 0.75 +0.0 1,608,305 - Pf1N1B4_1403 0.75 +0.1 1,608,305 - Pf1N1B4_1403 0.75 -0.5 1,608,305 - Pf1N1B4_1403 0.75 -0.7 1,608,305 - Pf1N1B4_1403 0.75 +0.3 1,608,487 + Pf1N1B4_1403 0.82 -0.6 1,608,727 + -1.0 1,608,727 + +0.1 1,608,727 + -1.7 1,608,728 - +0.2 1,608,728 - +0.1 1,608,745 + -0.7 1,608,745 + -0.7 1,608,745 + +0.6 1,608,746 - -1.3 1,608,986 + +1.0 1,608,997 + +0.3 1,609,050 + -0.8 1,609,367 + +0.0 1,609,367 + +0.7 1,609,367 + -0.1 1,609,368 - -0.8 1,609,434 - -1.0 1,609,434 - +0.3 1,609,434 - -0.3 1,609,516 + +0.2 1,609,517 - -2.3 1,609,575 + +0.3 1,609,627 + -2.0 1,609,718 + Pf1N1B4_1404 0.10 -1.7 1,609,718 + Pf1N1B4_1404 0.10 +1.6 1,609,718 + Pf1N1B4_1404 0.10 +1.0 1,609,718 + Pf1N1B4_1404 0.10 +0.0 1,609,719 - Pf1N1B4_1404 0.10 -1.0 1,609,719 - Pf1N1B4_1404 0.10 +0.9 1,609,719 - Pf1N1B4_1404 0.10 -1.0 1,609,719 - Pf1N1B4_1404 0.10 -0.3 1,609,719 - Pf1N1B4_1404 0.10 +1.9 1,609,868 + Pf1N1B4_1404 0.25 +1.3 1,609,868 + Pf1N1B4_1404 0.25 +1.3 1,609,868 + Pf1N1B4_1404 0.25 +0.6 1,609,869 - Pf1N1B4_1404 0.25 +0.7 1,609,869 - -0.3 1,609,869 - Pf1N1B4_1404 0.25 +0.5 1,609,869 - Pf1N1B4_1404 0.25 +1.3 1,609,869 - Pf1N1B4_1404 0.25 -0.1 1,609,869 - Pf1N1B4_1404 0.25 +0.2 1,609,961 + Pf1N1B4_1404 0.34 +0.7 1,609,961 + Pf1N1B4_1404 0.34 +1.6 1,609,961 + Pf1N1B4_1404 0.34 +0.2 1,609,961 + Pf1N1B4_1404 0.34 +1.1 1,609,962 - Pf1N1B4_1404 0.35 +0.1 1,609,962 - Pf1N1B4_1404 0.35 +0.9 1,609,962 - Pf1N1B4_1404 0.35 +0.5 1,609,962 - Pf1N1B4_1404 0.35 +2.9
Or see this region's nucleotide sequence