Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1330 and Pf1N1B4_1331 are separated by 242 nucleotides Pf1N1B4_1331 and Pf1N1B4_1332 overlap by 8 nucleotides
Pf1N1B4_1330 - hypothetical protein, at 1,525,225 to 1,525,881
_1330
Pf1N1B4_1331 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 1,526,124 to 1,527,335
_1331
Pf1N1B4_1332 - Cobalt-precorrin-6 synthase, anaerobic, at 1,527,328 to 1,528,425
_1332
Position (kb)
1526
1527
1528 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1525.145 kb on - strand at 1525.201 kb on - strand at 1525.201 kb on - strand at 1525.208 kb on - strand at 1525.236 kb on - strand at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.383 kb on + strand, within Pf1N1B4_1330 at 1525.384 kb on - strand, within Pf1N1B4_1330 at 1525.448 kb on + strand, within Pf1N1B4_1330 at 1525.464 kb on + strand, within Pf1N1B4_1330 at 1525.729 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.759 kb on + strand, within Pf1N1B4_1330 at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.882 kb on - strand at 1525.975 kb on + strand at 1526.016 kb on + strand at 1526.016 kb on + strand at 1526.339 kb on - strand, within Pf1N1B4_1331 at 1526.482 kb on + strand, within Pf1N1B4_1331 at 1526.483 kb on - strand, within Pf1N1B4_1331 at 1526.914 kb on + strand at 1526.915 kb on - strand, within Pf1N1B4_1331 at 1526.915 kb on - strand, within Pf1N1B4_1331 at 1526.915 kb on - strand, within Pf1N1B4_1331 at 1527.116 kb on - strand, within Pf1N1B4_1331 at 1527.116 kb on - strand, within Pf1N1B4_1331 at 1527.123 kb on - strand, within Pf1N1B4_1331 at 1527.123 kb on - strand, within Pf1N1B4_1331 at 1527.217 kb on - strand at 1527.299 kb on + strand at 1527.299 kb on + strand at 1527.300 kb on - strand at 1527.300 kb on - strand at 1527.757 kb on + strand, within Pf1N1B4_1332 at 1527.901 kb on + strand, within Pf1N1B4_1332 at 1527.902 kb on - strand, within Pf1N1B4_1332 at 1528.007 kb on - strand, within Pf1N1B4_1332 at 1528.007 kb on - strand, within Pf1N1B4_1332 at 1528.252 kb on - strand, within Pf1N1B4_1332
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,525,145 - +1.0 1,525,201 - +0.0 1,525,201 - +1.3 1,525,208 - -0.5 1,525,236 - +0.1 1,525,377 - Pf1N1B4_1330 0.23 +0.7 1,525,377 - Pf1N1B4_1330 0.23 -0.3 1,525,377 - Pf1N1B4_1330 0.23 -1.2 1,525,383 + Pf1N1B4_1330 0.24 -1.0 1,525,384 - Pf1N1B4_1330 0.24 +0.3 1,525,448 + Pf1N1B4_1330 0.34 +0.6 1,525,464 + Pf1N1B4_1330 0.36 -1.7 1,525,729 - Pf1N1B4_1330 0.77 +0.0 1,525,731 - Pf1N1B4_1330 0.77 +0.7 1,525,731 - Pf1N1B4_1330 0.77 +0.3 1,525,759 + Pf1N1B4_1330 0.81 -0.3 1,525,825 - +0.0 1,525,825 - -1.5 1,525,825 - +0.3 1,525,882 - +1.9 1,525,975 + -0.3 1,526,016 + -0.3 1,526,016 + +0.6 1,526,339 - Pf1N1B4_1331 0.18 +1.1 1,526,482 + Pf1N1B4_1331 0.30 +0.1 1,526,483 - Pf1N1B4_1331 0.30 +0.1 1,526,914 + +2.9 1,526,915 - Pf1N1B4_1331 0.65 +0.8 1,526,915 - Pf1N1B4_1331 0.65 +0.3 1,526,915 - Pf1N1B4_1331 0.65 +0.2 1,527,116 - Pf1N1B4_1331 0.82 -0.0 1,527,116 - Pf1N1B4_1331 0.82 +2.3 1,527,123 - Pf1N1B4_1331 0.82 +1.3 1,527,123 - Pf1N1B4_1331 0.82 +0.4 1,527,217 - -1.5 1,527,299 + -0.3 1,527,299 + +0.5 1,527,300 - -0.3 1,527,300 - +0.3 1,527,757 + Pf1N1B4_1332 0.39 +0.3 1,527,901 + Pf1N1B4_1332 0.52 +0.2 1,527,902 - Pf1N1B4_1332 0.52 +0.6 1,528,007 - Pf1N1B4_1332 0.62 -0.4 1,528,007 - Pf1N1B4_1332 0.62 +0.1 1,528,252 - Pf1N1B4_1332 0.84 -0.0
Or see this region's nucleotide sequence