Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1156 and Pf1N1B4_1157 are separated by 42 nucleotides Pf1N1B4_1157 and Pf1N1B4_1158 are separated by 12 nucleotides Pf1N1B4_1158 and Pf1N1B4_1159 are separated by 142 nucleotides Pf1N1B4_1159 and Pf1N1B4_1160 are separated by 3 nucleotides Pf1N1B4_1160 and Pf1N1B4_1161 are separated by 159 nucleotides
Pf1N1B4_1156 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 1,303,300 to 1,305,405
_1156
Pf1N1B4_1157 - hypothetical protein, at 1,305,448 to 1,305,588
_1157
Pf1N1B4_1158 - SSU ribosomal protein S15p (S13e), at 1,305,601 to 1,305,870
_1158
Pf1N1B4_1159 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 1,306,013 to 1,306,930
_1159
Pf1N1B4_1160 - Ribosome-binding factor A, at 1,306,934 to 1,307,335
_1160
Pf1N1B4_1161 - Translation initiation factor 2, at 1,307,495 to 1,310,020
_1161
Position (kb)
1306
1307 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1306.345 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.396 kb on - strand, within Pf1N1B4_1159 at 1306.469 kb on - strand, within Pf1N1B4_1159 at 1306.497 kb on - strand, within Pf1N1B4_1159 at 1306.521 kb on + strand, within Pf1N1B4_1159 at 1306.554 kb on + strand, within Pf1N1B4_1159 at 1306.729 kb on - strand, within Pf1N1B4_1159 at 1306.912 kb on + strand at 1306.913 kb on - strand at 1306.935 kb on + strand at 1307.351 kb on - strand at 1307.351 kb on - strand at 1307.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,306,345 - Pf1N1B4_1159 0.36 +0.2 1,306,396 - Pf1N1B4_1159 0.42 -0.4 1,306,396 - Pf1N1B4_1159 0.42 -1.1 1,306,396 - Pf1N1B4_1159 0.42 +0.7 1,306,396 - Pf1N1B4_1159 0.42 -1.8 1,306,396 - Pf1N1B4_1159 0.42 -0.8 1,306,469 - Pf1N1B4_1159 0.50 +0.7 1,306,497 - Pf1N1B4_1159 0.53 -1.2 1,306,521 + Pf1N1B4_1159 0.55 +0.5 1,306,554 + Pf1N1B4_1159 0.59 +2.8 1,306,729 - Pf1N1B4_1159 0.78 -2.3 1,306,912 + +1.2 1,306,913 - -1.1 1,306,935 + +0.7 1,307,351 - +0.8 1,307,351 - -0.1 1,307,497 - -0.4
Or see this region's nucleotide sequence