Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1124 and Pf1N1B4_1125 are separated by 10 nucleotides Pf1N1B4_1125 and Pf1N1B4_1126 are separated by 10 nucleotides Pf1N1B4_1126 and Pf1N1B4_1127 overlap by 1 nucleotides
Pf1N1B4_1124 - Dipeptide transport system permease protein DppB (TC 3.A.1.5.2), at 1,262,812 to 1,263,822
_1124
Pf1N1B4_1125 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 1,263,833 to 1,264,744
_1125
Pf1N1B4_1126 - Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2), at 1,264,755 to 1,265,723
_1126
Pf1N1B4_1127 - Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2), at 1,265,723 to 1,266,703
_1127
Position (kb)
1264
1265
1266 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1263.785 kb on - strand at 1263.848 kb on - strand at 1263.848 kb on - strand at 1263.879 kb on - strand at 1263.891 kb on - strand at 1263.891 kb on - strand at 1263.893 kb on - strand at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.361 kb on + strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.362 kb on - strand, within Pf1N1B4_1125 at 1264.410 kb on - strand, within Pf1N1B4_1125 at 1264.566 kb on + strand, within Pf1N1B4_1125 at 1264.566 kb on + strand, within Pf1N1B4_1125 at 1264.567 kb on - strand, within Pf1N1B4_1125 at 1264.567 kb on - strand, within Pf1N1B4_1125 at 1264.617 kb on - strand, within Pf1N1B4_1125 at 1264.617 kb on - strand, within Pf1N1B4_1125 at 1264.766 kb on - strand at 1264.766 kb on - strand at 1265.067 kb on + strand, within Pf1N1B4_1126 at 1265.067 kb on + strand, within Pf1N1B4_1126 at 1265.309 kb on - strand, within Pf1N1B4_1126 at 1265.434 kb on - strand, within Pf1N1B4_1126 at 1265.625 kb on + strand, within Pf1N1B4_1126 at 1265.625 kb on + strand, within Pf1N1B4_1126 at 1265.625 kb on + strand, within Pf1N1B4_1126 at 1265.626 kb on - strand, within Pf1N1B4_1126 at 1265.733 kb on + strand at 1265.733 kb on + strand at 1265.733 kb on + strand at 1265.734 kb on - strand at 1265.762 kb on + strand at 1265.763 kb on - strand at 1266.319 kb on - strand, within Pf1N1B4_1127 at 1266.319 kb on - strand, within Pf1N1B4_1127 at 1266.319 kb on - strand at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.341 kb on + strand, within Pf1N1B4_1127 at 1266.342 kb on - strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.404 kb on + strand, within Pf1N1B4_1127 at 1266.405 kb on - strand, within Pf1N1B4_1127 at 1266.405 kb on - strand, within Pf1N1B4_1127 at 1266.446 kb on + strand at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.446 kb on + strand at 1266.446 kb on + strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.447 kb on - strand, within Pf1N1B4_1127 at 1266.671 kb on + strand at 1266.671 kb on + strand at 1266.701 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,263,785 - -1.1 1,263,848 - +0.1 1,263,848 - +0.2 1,263,879 - -1.1 1,263,891 - +0.5 1,263,891 - +0.2 1,263,893 - +0.8 1,264,361 + Pf1N1B4_1125 0.58 +1.2 1,264,361 + Pf1N1B4_1125 0.58 -1.4 1,264,361 + Pf1N1B4_1125 0.58 -0.1 1,264,361 + Pf1N1B4_1125 0.58 -0.0 1,264,361 + Pf1N1B4_1125 0.58 +2.8 1,264,361 + Pf1N1B4_1125 0.58 -2.0 1,264,361 + Pf1N1B4_1125 0.58 +0.2 1,264,362 - Pf1N1B4_1125 0.58 +0.5 1,264,362 - Pf1N1B4_1125 0.58 +0.2 1,264,362 - Pf1N1B4_1125 0.58 -0.5 1,264,362 - Pf1N1B4_1125 0.58 +1.2 1,264,362 - Pf1N1B4_1125 0.58 +0.6 1,264,362 - Pf1N1B4_1125 0.58 +0.8 1,264,362 - Pf1N1B4_1125 0.58 +0.2 1,264,410 - Pf1N1B4_1125 0.63 +1.2 1,264,566 + Pf1N1B4_1125 0.80 +0.3 1,264,566 + Pf1N1B4_1125 0.80 +0.5 1,264,567 - Pf1N1B4_1125 0.80 +0.2 1,264,567 - Pf1N1B4_1125 0.80 +1.9 1,264,617 - Pf1N1B4_1125 0.86 +0.8 1,264,617 - Pf1N1B4_1125 0.86 +0.5 1,264,766 - -0.4 1,264,766 - -0.4 1,265,067 + Pf1N1B4_1126 0.32 -0.8 1,265,067 + Pf1N1B4_1126 0.32 +0.2 1,265,309 - Pf1N1B4_1126 0.57 -1.4 1,265,434 - Pf1N1B4_1126 0.70 -1.1 1,265,625 + Pf1N1B4_1126 0.90 -0.1 1,265,625 + Pf1N1B4_1126 0.90 -0.5 1,265,625 + Pf1N1B4_1126 0.90 -1.3 1,265,626 - Pf1N1B4_1126 0.90 +0.5 1,265,733 + -0.6 1,265,733 + +0.4 1,265,733 + +0.6 1,265,734 - +2.0 1,265,762 + +1.2 1,265,763 - -0.8 1,266,319 - Pf1N1B4_1127 0.61 -0.4 1,266,319 - Pf1N1B4_1127 0.61 +0.5 1,266,319 - +0.2 1,266,341 + Pf1N1B4_1127 0.63 +0.5 1,266,341 + Pf1N1B4_1127 0.63 -1.1 1,266,341 + Pf1N1B4_1127 0.63 +0.6 1,266,341 + Pf1N1B4_1127 0.63 -0.3 1,266,342 - Pf1N1B4_1127 0.63 -1.4 1,266,404 + Pf1N1B4_1127 0.69 +0.6 1,266,404 + Pf1N1B4_1127 0.69 -0.0 1,266,404 + Pf1N1B4_1127 0.69 +0.8 1,266,404 + Pf1N1B4_1127 0.69 +0.3 1,266,404 + Pf1N1B4_1127 0.69 +0.2 1,266,404 + Pf1N1B4_1127 0.69 -0.6 1,266,405 - Pf1N1B4_1127 0.70 +1.6 1,266,405 - Pf1N1B4_1127 0.70 +0.3 1,266,446 + +1.2 1,266,446 + Pf1N1B4_1127 0.74 -0.5 1,266,446 + Pf1N1B4_1127 0.74 +1.7 1,266,446 + Pf1N1B4_1127 0.74 -1.1 1,266,446 + Pf1N1B4_1127 0.74 +0.8 1,266,446 + Pf1N1B4_1127 0.74 +1.2 1,266,446 + Pf1N1B4_1127 0.74 -1.1 1,266,446 + +2.2 1,266,446 + Pf1N1B4_1127 0.74 +1.2 1,266,447 - Pf1N1B4_1127 0.74 -0.1 1,266,447 - Pf1N1B4_1127 0.74 -0.3 1,266,447 - Pf1N1B4_1127 0.74 +0.4 1,266,447 - Pf1N1B4_1127 0.74 -0.4 1,266,447 - Pf1N1B4_1127 0.74 +0.6 1,266,671 + -0.9 1,266,671 + +1.8 1,266,701 + -0.0
Or see this region's nucleotide sequence