Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_108 and Pf1N1B4_109 are separated by 82 nucleotides Pf1N1B4_109 and Pf1N1B4_110 are separated by 17 nucleotides Pf1N1B4_110 and Pf1N1B4_111 are separated by 226 nucleotides
Pf1N1B4_108 - FIG00954456: hypothetical protein, at 113,219 to 114,298
_108
Pf1N1B4_109 - hypothetical protein, at 114,381 to 114,536
_109
Pf1N1B4_110 - Queuosine biosynthesis QueD, PTPS-I, at 114,554 to 114,910
_110
Pf1N1B4_111 - Possible oxidoreductase, at 115,137 to 115,961
_111
Position (kb)
114
115 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 113.961 kb on - strand, within Pf1N1B4_108 at 113.979 kb on - strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.116 kb on + strand, within Pf1N1B4_108 at 114.200 kb on + strand at 114.201 kb on - strand at 114.234 kb on + strand at 114.315 kb on + strand at 114.316 kb on - strand at 114.494 kb on - strand, within Pf1N1B4_109 at 114.740 kb on + strand, within Pf1N1B4_110 at 114.740 kb on + strand, within Pf1N1B4_110 at 114.741 kb on - strand, within Pf1N1B4_110 at 114.757 kb on + strand, within Pf1N1B4_110 at 114.758 kb on - strand at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.758 kb on - strand, within Pf1N1B4_110 at 114.761 kb on + strand, within Pf1N1B4_110 at 114.761 kb on + strand, within Pf1N1B4_110 at 114.762 kb on - strand, within Pf1N1B4_110 at 114.762 kb on - strand, within Pf1N1B4_110 at 114.762 kb on - strand at 114.896 kb on + strand at 115.064 kb on - strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.099 kb on + strand at 115.100 kb on - strand at 115.100 kb on - strand at 115.100 kb on - strand at 115.165 kb on - strand at 115.198 kb on + strand at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.251 kb on + strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.252 kb on - strand, within Pf1N1B4_111 at 115.255 kb on + strand, within Pf1N1B4_111 at 115.255 kb on + strand at 115.255 kb on + strand, within Pf1N1B4_111 at 115.255 kb on + strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.256 kb on - strand, within Pf1N1B4_111 at 115.303 kb on - strand, within Pf1N1B4_111 at 115.303 kb on - strand, within Pf1N1B4_111 at 115.321 kb on - strand, within Pf1N1B4_111 at 115.363 kb on + strand, within Pf1N1B4_111 at 115.482 kb on + strand, within Pf1N1B4_111 at 115.587 kb on + strand, within Pf1N1B4_111 at 115.615 kb on - strand, within Pf1N1B4_111 at 115.615 kb on - strand, within Pf1N1B4_111 at 115.616 kb on + strand at 115.616 kb on + strand, within Pf1N1B4_111 at 115.617 kb on - strand, within Pf1N1B4_111 at 115.617 kb on - strand, within Pf1N1B4_111 at 115.617 kb on - strand, within Pf1N1B4_111 at 115.665 kb on + strand, within Pf1N1B4_111 at 115.665 kb on + strand, within Pf1N1B4_111 at 115.665 kb on + strand, within Pf1N1B4_111 at 115.665 kb on + strand, within Pf1N1B4_111 at 115.666 kb on - strand at 115.708 kb on - strand, within Pf1N1B4_111
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 113,961 - Pf1N1B4_108 0.69 -0.3 113,979 - Pf1N1B4_108 0.70 +1.0 114,116 + Pf1N1B4_108 0.83 +1.7 114,116 + Pf1N1B4_108 0.83 +0.7 114,116 + Pf1N1B4_108 0.83 +1.2 114,116 + Pf1N1B4_108 0.83 +1.5 114,200 + -1.3 114,201 - +0.0 114,234 + +0.7 114,315 + -0.0 114,316 - +2.1 114,494 - Pf1N1B4_109 0.72 +0.2 114,740 + Pf1N1B4_110 0.52 -0.1 114,740 + Pf1N1B4_110 0.52 -1.3 114,741 - Pf1N1B4_110 0.52 -0.6 114,757 + Pf1N1B4_110 0.57 -0.8 114,758 - +0.2 114,758 - Pf1N1B4_110 0.57 -1.3 114,758 - Pf1N1B4_110 0.57 -0.7 114,758 - Pf1N1B4_110 0.57 -0.3 114,758 - Pf1N1B4_110 0.57 +0.3 114,761 + Pf1N1B4_110 0.58 -0.6 114,761 + Pf1N1B4_110 0.58 -0.4 114,762 - Pf1N1B4_110 0.58 -1.1 114,762 - Pf1N1B4_110 0.58 -0.5 114,762 - +1.3 114,896 + -1.3 115,064 - +0.7 115,099 + +2.3 115,099 + -1.6 115,099 + -0.7 115,099 + -0.0 115,099 + +1.1 115,099 + -0.8 115,100 - -0.7 115,100 - +0.7 115,100 - -1.3 115,165 - +0.4 115,198 + -0.3 115,251 + Pf1N1B4_111 0.14 +0.2 115,251 + Pf1N1B4_111 0.14 -0.7 115,251 + Pf1N1B4_111 0.14 +1.6 115,251 + Pf1N1B4_111 0.14 +0.5 115,252 - Pf1N1B4_111 0.14 +0.7 115,252 - Pf1N1B4_111 0.14 +1.7 115,252 - Pf1N1B4_111 0.14 -0.1 115,255 + Pf1N1B4_111 0.14 +0.0 115,255 + +0.7 115,255 + Pf1N1B4_111 0.14 -2.3 115,255 + Pf1N1B4_111 0.14 +0.4 115,256 - Pf1N1B4_111 0.14 +0.5 115,256 - Pf1N1B4_111 0.14 -0.8 115,256 - Pf1N1B4_111 0.14 +0.2 115,256 - Pf1N1B4_111 0.14 -0.1 115,256 - Pf1N1B4_111 0.14 +0.3 115,303 - Pf1N1B4_111 0.20 +1.3 115,303 - Pf1N1B4_111 0.20 +0.7 115,321 - Pf1N1B4_111 0.22 -1.1 115,363 + Pf1N1B4_111 0.27 +0.9 115,482 + Pf1N1B4_111 0.42 -1.1 115,587 + Pf1N1B4_111 0.55 +3.1 115,615 - Pf1N1B4_111 0.58 +0.9 115,615 - Pf1N1B4_111 0.58 +0.1 115,616 + +2.7 115,616 + Pf1N1B4_111 0.58 +1.7 115,617 - Pf1N1B4_111 0.58 -0.5 115,617 - Pf1N1B4_111 0.58 +0.6 115,617 - Pf1N1B4_111 0.58 +0.2 115,665 + Pf1N1B4_111 0.64 -0.5 115,665 + Pf1N1B4_111 0.64 -0.5 115,665 + Pf1N1B4_111 0.64 +0.2 115,665 + Pf1N1B4_111 0.64 +0.3 115,666 - +1.0 115,708 - Pf1N1B4_111 0.69 -0.3
Or see this region's nucleotide sequence