Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1056 and Pf1N1B4_1057 are separated by 181 nucleotides Pf1N1B4_1057 and Pf1N1B4_1058 are separated by 9 nucleotides
Pf1N1B4_1056 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,191,106 to 1,192,014
_1056
Pf1N1B4_1057 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25), at 1,192,196 to 1,194,094
_1057
Pf1N1B4_1058 - Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4), at 1,194,104 to 1,195,021
_1058
Position (kb)
1192
1193
1194
1195 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1191.359 kb on - strand, within Pf1N1B4_1056 at 1191.370 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.421 kb on + strand, within Pf1N1B4_1056 at 1191.422 kb on - strand, within Pf1N1B4_1056 at 1191.422 kb on - strand, within Pf1N1B4_1056 at 1191.548 kb on - strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.680 kb on + strand, within Pf1N1B4_1056 at 1191.681 kb on - strand, within Pf1N1B4_1056 at 1191.681 kb on - strand, within Pf1N1B4_1056 at 1191.901 kb on + strand, within Pf1N1B4_1056 at 1191.902 kb on - strand, within Pf1N1B4_1056 at 1191.997 kb on + strand at 1191.997 kb on + strand at 1191.997 kb on + strand at 1191.998 kb on - strand at 1192.180 kb on + strand at 1192.198 kb on - strand at 1192.213 kb on + strand at 1192.345 kb on - strand at 1192.395 kb on + strand, within Pf1N1B4_1057 at 1192.396 kb on - strand, within Pf1N1B4_1057 at 1192.751 kb on + strand, within Pf1N1B4_1057 at 1192.752 kb on - strand, within Pf1N1B4_1057 at 1192.752 kb on - strand, within Pf1N1B4_1057 at 1193.038 kb on - strand, within Pf1N1B4_1057 at 1193.374 kb on - strand, within Pf1N1B4_1057 at 1194.105 kb on + strand at 1195.026 kb on - strand at 1195.026 kb on - strand at 1195.043 kb on + strand at 1195.043 kb on + strand at 1195.043 kb on + strand at 1195.043 kb on + strand at 1195.044 kb on - strand at 1195.044 kb on - strand at 1195.044 kb on - strand at 1195.044 kb on - strand at 1195.044 kb on - strand at 1195.044 kb on - strand at 1195.081 kb on + strand at 1195.081 kb on + strand at 1195.081 kb on + strand at 1195.081 kb on + strand at 1195.081 kb on + strand at 1195.081 kb on + strand at 1195.082 kb on - strand at 1195.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,191,359 - Pf1N1B4_1056 0.28 -1.6 1,191,370 + Pf1N1B4_1056 0.29 -1.6 1,191,421 + Pf1N1B4_1056 0.35 -1.4 1,191,421 + Pf1N1B4_1056 0.35 -0.3 1,191,421 + Pf1N1B4_1056 0.35 +1.5 1,191,421 + Pf1N1B4_1056 0.35 -0.5 1,191,422 - Pf1N1B4_1056 0.35 +1.0 1,191,422 - Pf1N1B4_1056 0.35 +1.2 1,191,548 - Pf1N1B4_1056 0.49 +1.8 1,191,680 + Pf1N1B4_1056 0.63 +0.8 1,191,680 + Pf1N1B4_1056 0.63 +1.6 1,191,680 + Pf1N1B4_1056 0.63 -0.4 1,191,681 - Pf1N1B4_1056 0.63 +0.4 1,191,681 - Pf1N1B4_1056 0.63 -1.4 1,191,901 + Pf1N1B4_1056 0.87 -1.2 1,191,902 - Pf1N1B4_1056 0.88 -1.2 1,191,997 + -0.9 1,191,997 + -0.4 1,191,997 + -1.2 1,191,998 - +2.0 1,192,180 + +0.6 1,192,198 - -0.4 1,192,213 + +0.2 1,192,345 - -0.8 1,192,395 + Pf1N1B4_1057 0.10 +0.4 1,192,396 - Pf1N1B4_1057 0.11 -0.2 1,192,751 + Pf1N1B4_1057 0.29 -1.9 1,192,752 - Pf1N1B4_1057 0.29 -0.8 1,192,752 - Pf1N1B4_1057 0.29 +0.6 1,193,038 - Pf1N1B4_1057 0.44 -0.7 1,193,374 - Pf1N1B4_1057 0.62 -2.5 1,194,105 + -1.4 1,195,026 - +0.0 1,195,026 - -0.2 1,195,043 + -0.2 1,195,043 + -1.4 1,195,043 + -2.4 1,195,043 + -1.3 1,195,044 - -0.2 1,195,044 - -1.1 1,195,044 - -1.0 1,195,044 - +0.2 1,195,044 - +0.2 1,195,044 - +0.5 1,195,081 + -0.4 1,195,081 + -0.8 1,195,081 + +0.8 1,195,081 + -1.4 1,195,081 + +0.4 1,195,081 + +0.7 1,195,082 - -0.1 1,195,082 - +0.4
Or see this region's nucleotide sequence