Experiment: formate McAA thiamine dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05710 and MMJJ_RS05715 are separated by 48 nucleotides MMJJ_RS05715 and MMJJ_RS05720 overlap by 14 nucleotides
MMJJ_RS05710: MMJJ_RS05710 - single-stranded-DNA-specific exonuclease RecJ, at 1,063,211 to 1,064,614
_RS05710
MMJJ_RS05715: MMJJ_RS05715 - phosphoadenosine phosphosulfate reductase family protein, at 1,064,663 to 1,065,949
_RS05715
MMJJ_RS05720: MMJJ_RS05720 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 1,065,936 to 1,067,735
_RS05720
Position (kb)
1064
1065
1066 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1063.842 kb on + strand, within MMJJ_RS05710 at 1063.891 kb on - strand, within MMJJ_RS05710 at 1063.891 kb on - strand, within MMJJ_RS05710 at 1063.932 kb on - strand, within MMJJ_RS05710 at 1063.946 kb on - strand, within MMJJ_RS05710 at 1064.209 kb on - strand, within MMJJ_RS05710 at 1064.314 kb on - strand, within MMJJ_RS05710 at 1064.314 kb on - strand, within MMJJ_RS05710 at 1064.442 kb on - strand, within MMJJ_RS05710 at 1064.507 kb on - strand at 1064.507 kb on - strand at 1064.594 kb on - strand at 1064.595 kb on + strand at 1064.623 kb on + strand at 1064.639 kb on + strand at 1064.655 kb on - strand at 1064.663 kb on + strand at 1064.668 kb on - strand at 1064.669 kb on + strand at 1064.703 kb on - strand at 1064.752 kb on + strand at 1064.798 kb on - strand, within MMJJ_RS05715 at 1064.798 kb on - strand, within MMJJ_RS05715 at 1064.799 kb on + strand at 1064.847 kb on - strand, within MMJJ_RS05715 at 1064.879 kb on - strand, within MMJJ_RS05715 at 1064.880 kb on + strand, within MMJJ_RS05715 at 1064.880 kb on + strand, within MMJJ_RS05715 at 1065.070 kb on + strand, within MMJJ_RS05715 at 1065.096 kb on - strand, within MMJJ_RS05715 at 1065.123 kb on - strand, within MMJJ_RS05715 at 1065.129 kb on + strand, within MMJJ_RS05715 at 1065.134 kb on + strand, within MMJJ_RS05715 at 1065.141 kb on + strand, within MMJJ_RS05715 at 1065.265 kb on - strand, within MMJJ_RS05715 at 1065.266 kb on + strand, within MMJJ_RS05715 at 1065.341 kb on - strand, within MMJJ_RS05715 at 1065.387 kb on - strand, within MMJJ_RS05715 at 1065.466 kb on - strand, within MMJJ_RS05715 at 1065.471 kb on - strand, within MMJJ_RS05715 at 1065.488 kb on - strand, within MMJJ_RS05715 at 1065.493 kb on + strand, within MMJJ_RS05715 at 1065.493 kb on + strand, within MMJJ_RS05715 at 1065.523 kb on - strand, within MMJJ_RS05715 at 1065.657 kb on + strand, within MMJJ_RS05715 at 1065.660 kb on - strand, within MMJJ_RS05715 at 1065.661 kb on + strand, within MMJJ_RS05715 at 1065.676 kb on - strand, within MMJJ_RS05715 at 1065.677 kb on + strand, within MMJJ_RS05715 at 1065.677 kb on + strand, within MMJJ_RS05715 at 1065.677 kb on + strand, within MMJJ_RS05715 at 1065.677 kb on + strand, within MMJJ_RS05715 at 1065.678 kb on - strand, within MMJJ_RS05715 at 1065.678 kb on - strand, within MMJJ_RS05715 at 1065.678 kb on - strand, within MMJJ_RS05715 at 1065.678 kb on - strand, within MMJJ_RS05715 at 1065.678 kb on - strand, within MMJJ_RS05715 at 1065.679 kb on + strand, within MMJJ_RS05715 at 1065.679 kb on + strand, within MMJJ_RS05715 at 1065.679 kb on + strand, within MMJJ_RS05715 at 1065.680 kb on - strand, within MMJJ_RS05715 at 1065.680 kb on - strand, within MMJJ_RS05715 at 1065.680 kb on - strand, within MMJJ_RS05715 at 1065.680 kb on - strand, within MMJJ_RS05715 at 1065.680 kb on - strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.681 kb on + strand, within MMJJ_RS05715 at 1065.682 kb on - strand, within MMJJ_RS05715 at 1065.692 kb on - strand, within MMJJ_RS05715 at 1065.692 kb on - strand, within MMJJ_RS05715 at 1065.693 kb on + strand, within MMJJ_RS05715 at 1065.763 kb on + strand, within MMJJ_RS05715 at 1065.774 kb on + strand, within MMJJ_RS05715 at 1065.804 kb on + strand, within MMJJ_RS05715 at 1065.804 kb on + strand, within MMJJ_RS05715 at 1065.804 kb on + strand, within MMJJ_RS05715 at 1065.809 kb on + strand, within MMJJ_RS05715 at 1065.815 kb on - strand, within MMJJ_RS05715 at 1065.879 kb on - strand at 1065.895 kb on - strand at 1065.903 kb on - strand at 1065.903 kb on - strand at 1065.903 kb on - strand at 1065.903 kb on - strand at 1065.904 kb on + strand at 1065.904 kb on + strand at 1065.937 kb on - strand at 1066.063 kb on + strand at 1066.782 kb on - strand, within MMJJ_RS05720 at 1066.846 kb on - strand, within MMJJ_RS05720 at 1066.847 kb on + strand, within MMJJ_RS05720 at 1066.849 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA thiamine dropout remove 1,063,842 + MMJJ_RS05710 0.45 -1.1 1,063,891 - MMJJ_RS05710 0.48 -2.4 1,063,891 - MMJJ_RS05710 0.48 -1.8 1,063,932 - MMJJ_RS05710 0.51 -3.0 1,063,946 - MMJJ_RS05710 0.52 -2.4 1,064,209 - MMJJ_RS05710 0.71 -0.8 1,064,314 - MMJJ_RS05710 0.79 +0.3 1,064,314 - MMJJ_RS05710 0.79 +0.2 1,064,442 - MMJJ_RS05710 0.88 +0.3 1,064,507 - -1.4 1,064,507 - -0.3 1,064,594 - -0.1 1,064,595 + -2.1 1,064,623 + -2.4 1,064,639 + -0.0 1,064,655 - -0.7 1,064,663 + +0.6 1,064,668 - +1.6 1,064,669 + +0.3 1,064,703 - -0.5 1,064,752 + -2.7 1,064,798 - MMJJ_RS05715 0.10 -0.8 1,064,798 - MMJJ_RS05715 0.10 +0.8 1,064,799 + -0.3 1,064,847 - MMJJ_RS05715 0.14 +1.1 1,064,879 - MMJJ_RS05715 0.17 +0.5 1,064,880 + MMJJ_RS05715 0.17 +0.5 1,064,880 + MMJJ_RS05715 0.17 +0.9 1,065,070 + MMJJ_RS05715 0.32 -0.5 1,065,096 - MMJJ_RS05715 0.34 +1.5 1,065,123 - MMJJ_RS05715 0.36 +0.1 1,065,129 + MMJJ_RS05715 0.36 +1.4 1,065,134 + MMJJ_RS05715 0.37 +0.3 1,065,141 + MMJJ_RS05715 0.37 +0.8 1,065,265 - MMJJ_RS05715 0.47 -0.3 1,065,266 + MMJJ_RS05715 0.47 +1.8 1,065,341 - MMJJ_RS05715 0.53 +1.0 1,065,387 - MMJJ_RS05715 0.56 -0.9 1,065,466 - MMJJ_RS05715 0.62 -1.1 1,065,471 - MMJJ_RS05715 0.63 +1.3 1,065,488 - MMJJ_RS05715 0.64 +0.8 1,065,493 + MMJJ_RS05715 0.64 +1.2 1,065,493 + MMJJ_RS05715 0.64 +0.7 1,065,523 - MMJJ_RS05715 0.67 +1.2 1,065,657 + MMJJ_RS05715 0.77 -1.2 1,065,660 - MMJJ_RS05715 0.77 -2.2 1,065,661 + MMJJ_RS05715 0.78 -0.3 1,065,676 - MMJJ_RS05715 0.79 +0.6 1,065,677 + MMJJ_RS05715 0.79 -0.4 1,065,677 + MMJJ_RS05715 0.79 +0.6 1,065,677 + MMJJ_RS05715 0.79 -1.1 1,065,677 + MMJJ_RS05715 0.79 -1.6 1,065,678 - MMJJ_RS05715 0.79 -2.7 1,065,678 - MMJJ_RS05715 0.79 +0.1 1,065,678 - MMJJ_RS05715 0.79 +0.0 1,065,678 - MMJJ_RS05715 0.79 -0.5 1,065,678 - MMJJ_RS05715 0.79 -0.2 1,065,679 + MMJJ_RS05715 0.79 -2.7 1,065,679 + MMJJ_RS05715 0.79 +0.3 1,065,679 + MMJJ_RS05715 0.79 +1.0 1,065,680 - MMJJ_RS05715 0.79 -0.1 1,065,680 - MMJJ_RS05715 0.79 +0.3 1,065,680 - MMJJ_RS05715 0.79 +1.4 1,065,680 - MMJJ_RS05715 0.79 +3.8 1,065,680 - MMJJ_RS05715 0.79 -0.3 1,065,681 + MMJJ_RS05715 0.79 -1.5 1,065,681 + MMJJ_RS05715 0.79 +0.2 1,065,681 + MMJJ_RS05715 0.79 -0.2 1,065,681 + MMJJ_RS05715 0.79 -0.0 1,065,681 + MMJJ_RS05715 0.79 +1.1 1,065,681 + MMJJ_RS05715 0.79 +0.9 1,065,681 + MMJJ_RS05715 0.79 +0.3 1,065,681 + MMJJ_RS05715 0.79 -0.8 1,065,682 - MMJJ_RS05715 0.79 +0.6 1,065,692 - MMJJ_RS05715 0.80 -0.4 1,065,692 - MMJJ_RS05715 0.80 -1.0 1,065,693 + MMJJ_RS05715 0.80 +0.5 1,065,763 + MMJJ_RS05715 0.85 +0.6 1,065,774 + MMJJ_RS05715 0.86 +1.0 1,065,804 + MMJJ_RS05715 0.89 +1.1 1,065,804 + MMJJ_RS05715 0.89 +1.9 1,065,804 + MMJJ_RS05715 0.89 -1.5 1,065,809 + MMJJ_RS05715 0.89 +0.7 1,065,815 - MMJJ_RS05715 0.90 +0.4 1,065,879 - -1.3 1,065,895 - +1.4 1,065,903 - +0.6 1,065,903 - -0.8 1,065,903 - -0.1 1,065,903 - +0.3 1,065,904 + +0.7 1,065,904 + -0.1 1,065,937 - +0.1 1,066,063 + -2.4 1,066,782 - MMJJ_RS05720 0.47 -1.1 1,066,846 - MMJJ_RS05720 0.51 -2.6 1,066,847 + MMJJ_RS05720 0.51 -0.6 1,066,849 + -1.8
Or see this region's nucleotide sequence