Experiment: PYE with Benzethonium chloride 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00823 and CCNA_03941 overlap by 697 nucleotides CCNA_03941 and CCNA_00824 are separated by 415 nucleotides CCNA_00824 and CCNA_00825 are separated by 162 nucleotides CCNA_00825 and CCNA_00826 overlap by 4 nucleotides
CCNA_00823: CCNA_00823 - LuxR-like DNA-binding protein, at 884,441 to 885,244
_00823
CCNA_03941: CCNA_03941 - hypothetical protein, at 884,548 to 884,910
_03941
CCNA_00824: CCNA_00824 - GntR-family transcriptional regulator, at 885,326 to 886,054
_00824
CCNA_00825: CCNA_00825 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 886,217 to 886,825
_00825
CCNA_00826: CCNA_00826 - 2-dehydro-3-deoxygalactonokinase, at 886,822 to 887,742
_00826
Position (kb) 885
886
887 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 884.348 kb on - strand at 884.348 kb on - strand at 884.354 kb on - strand at 884.354 kb on - strand at 884.369 kb on - strand at 884.389 kb on + strand at 884.389 kb on + strand at 884.389 kb on + strand at 884.389 kb on + strand at 884.389 kb on + strand at 884.389 kb on + strand at 884.397 kb on - strand at 884.398 kb on + strand at 884.398 kb on + strand at 884.399 kb on + strand at 884.399 kb on + strand at 884.408 kb on + strand at 884.416 kb on - strand at 884.421 kb on - strand at 884.435 kb on - strand at 884.455 kb on - strand at 884.463 kb on + strand at 884.518 kb on - strand at 884.539 kb on + strand, within CCNA_00823 at 884.562 kb on - strand at 884.576 kb on - strand at 884.590 kb on - strand at 884.666 kb on + strand at 884.669 kb on - strand at 884.669 kb on - strand at 884.738 kb on + strand at 884.789 kb on + strand at 884.814 kb on + strand at 884.821 kb on + strand at 884.847 kb on - strand at 884.848 kb on - strand at 884.877 kb on + strand at 884.906 kb on + strand at 884.907 kb on - strand at 884.932 kb on + strand at 884.938 kb on - strand at 884.938 kb on - strand at 884.940 kb on - strand at 884.953 kb on + strand at 884.964 kb on + strand at 884.964 kb on + strand at 884.977 kb on - strand at 885.008 kb on + strand at 885.016 kb on - strand at 885.016 kb on - strand at 885.078 kb on + strand at 885.086 kb on - strand at 885.099 kb on + strand at 885.107 kb on - strand at 885.113 kb on - strand at 885.115 kb on + strand at 885.115 kb on + strand at 885.115 kb on + strand at 885.123 kb on - strand at 885.123 kb on - strand at 885.196 kb on + strand at 885.203 kb on - strand at 885.214 kb on + strand at 885.223 kb on + strand at 885.227 kb on + strand at 885.264 kb on + strand at 885.274 kb on - strand at 885.325 kb on + strand at 885.463 kb on - strand, within CCNA_00824 at 885.482 kb on - strand, within CCNA_00824 at 885.486 kb on + strand, within CCNA_00824 at 885.494 kb on - strand, within CCNA_00824 at 885.534 kb on + strand, within CCNA_00824 at 885.534 kb on + strand, within CCNA_00824 at 885.542 kb on - strand, within CCNA_00824 at 885.542 kb on - strand, within CCNA_00824 at 885.542 kb on - strand, within CCNA_00824 at 885.542 kb on - strand, within CCNA_00824 at 885.542 kb on - strand, within CCNA_00824 at 885.542 kb on - strand, within CCNA_00824 at 885.542 kb on - strand, within CCNA_00824 at 885.543 kb on + strand, within CCNA_00824 at 885.567 kb on + strand, within CCNA_00824 at 885.664 kb on - strand, within CCNA_00824 at 885.664 kb on - strand, within CCNA_00824 at 885.694 kb on - strand, within CCNA_00824 at 885.792 kb on + strand, within CCNA_00824 at 885.795 kb on - strand, within CCNA_00824 at 885.795 kb on - strand, within CCNA_00824 at 885.795 kb on - strand, within CCNA_00824 at 885.813 kb on - strand, within CCNA_00824 at 885.818 kb on - strand, within CCNA_00824 at 885.819 kb on + strand, within CCNA_00824 at 885.831 kb on + strand, within CCNA_00824 at 885.831 kb on + strand, within CCNA_00824 at 885.839 kb on - strand, within CCNA_00824 at 885.849 kb on + strand, within CCNA_00824 at 885.852 kb on - strand, within CCNA_00824 at 885.862 kb on - strand, within CCNA_00824 at 885.867 kb on + strand, within CCNA_00824 at 885.867 kb on + strand, within CCNA_00824 at 885.880 kb on - strand, within CCNA_00824 at 885.880 kb on - strand, within CCNA_00824 at 885.927 kb on + strand, within CCNA_00824 at 885.937 kb on + strand, within CCNA_00824 at 885.944 kb on - strand, within CCNA_00824 at 885.945 kb on - strand, within CCNA_00824 at 885.946 kb on + strand, within CCNA_00824 at 886.018 kb on + strand at 886.018 kb on + strand at 886.018 kb on + strand at 886.018 kb on + strand at 886.019 kb on + strand at 886.024 kb on + strand at 886.034 kb on - strand at 886.038 kb on - strand at 886.049 kb on - strand at 886.092 kb on - strand at 886.099 kb on + strand at 886.099 kb on + strand at 886.107 kb on - strand at 886.107 kb on - strand at 886.109 kb on + strand at 886.113 kb on + strand at 886.118 kb on + strand at 886.121 kb on - strand at 886.146 kb on + strand at 886.146 kb on + strand at 886.146 kb on + strand at 886.148 kb on + strand at 886.154 kb on - strand at 886.154 kb on - strand at 886.154 kb on - strand at 886.154 kb on - strand at 886.156 kb on - strand at 886.294 kb on - strand, within CCNA_00825 at 886.336 kb on + strand, within CCNA_00825 at 886.355 kb on + strand, within CCNA_00825 at 886.359 kb on - strand, within CCNA_00825 at 886.368 kb on + strand, within CCNA_00825 at 886.373 kb on + strand, within CCNA_00825 at 886.376 kb on - strand, within CCNA_00825 at 886.388 kb on + strand, within CCNA_00825 at 886.388 kb on + strand, within CCNA_00825 at 886.398 kb on + strand, within CCNA_00825 at 886.406 kb on - strand, within CCNA_00825 at 886.407 kb on + strand, within CCNA_00825 at 886.407 kb on + strand, within CCNA_00825 at 886.407 kb on + strand, within CCNA_00825 at 886.407 kb on + strand, within CCNA_00825 at 886.412 kb on + strand, within CCNA_00825 at 886.415 kb on - strand, within CCNA_00825 at 886.415 kb on - strand, within CCNA_00825 at 886.447 kb on + strand, within CCNA_00825 at 886.484 kb on + strand, within CCNA_00825 at 886.540 kb on + strand, within CCNA_00825 at 886.568 kb on - strand, within CCNA_00825 at 886.569 kb on - strand, within CCNA_00825 at 886.619 kb on + strand, within CCNA_00825 at 886.619 kb on + strand, within CCNA_00825 at 886.624 kb on + strand, within CCNA_00825 at 886.627 kb on - strand, within CCNA_00825 at 886.627 kb on - strand, within CCNA_00825 at 886.627 kb on - strand, within CCNA_00825 at 886.627 kb on - strand, within CCNA_00825 at 886.643 kb on - strand, within CCNA_00825 at 886.643 kb on - strand, within CCNA_00825 at 886.652 kb on + strand, within CCNA_00825 at 886.660 kb on - strand, within CCNA_00825 at 886.662 kb on - strand, within CCNA_00825 at 886.670 kb on - strand, within CCNA_00825 at 886.687 kb on + strand, within CCNA_00825 at 886.693 kb on + strand, within CCNA_00825 at 886.693 kb on + strand, within CCNA_00825 at 886.701 kb on - strand, within CCNA_00825 at 886.703 kb on + strand, within CCNA_00825 at 886.706 kb on - strand, within CCNA_00825 at 886.711 kb on - strand, within CCNA_00825 at 886.711 kb on - strand, within CCNA_00825 at 886.734 kb on + strand, within CCNA_00825 at 886.734 kb on + strand, within CCNA_00825 at 886.737 kb on - strand, within CCNA_00825 at 886.739 kb on + strand, within CCNA_00825 at 886.793 kb on + strand at 886.803 kb on - strand at 886.805 kb on + strand at 886.805 kb on + strand at 886.808 kb on - strand at 886.813 kb on - strand at 886.813 kb on - strand at 886.813 kb on - strand at 886.834 kb on + strand at 886.847 kb on - strand at 886.877 kb on + strand at 886.921 kb on + strand, within CCNA_00826 at 886.929 kb on - strand, within CCNA_00826 at 886.929 kb on - strand, within CCNA_00826 at 886.929 kb on - strand, within CCNA_00826 at 886.947 kb on + strand, within CCNA_00826 at 886.964 kb on + strand at 886.970 kb on + strand, within CCNA_00826 at 886.974 kb on + strand, within CCNA_00826 at 886.980 kb on + strand, within CCNA_00826 at 886.980 kb on + strand, within CCNA_00826 at 886.980 kb on - strand, within CCNA_00826 at 886.982 kb on - strand, within CCNA_00826 at 886.985 kb on + strand, within CCNA_00826 at 886.985 kb on + strand, within CCNA_00826 at 886.985 kb on + strand, within CCNA_00826 at 886.985 kb on + strand, within CCNA_00826 at 886.985 kb on + strand, within CCNA_00826 at 886.993 kb on - strand, within CCNA_00826 at 886.993 kb on - strand, within CCNA_00826 at 886.993 kb on - strand, within CCNA_00826 at 886.993 kb on - strand, within CCNA_00826 at 887.007 kb on + strand, within CCNA_00826 at 887.015 kb on - strand, within CCNA_00826 at 887.047 kb on - strand, within CCNA_00826
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Benzethonium chloride 0.001 mM remove 884,348 - +1.1 884,348 - +1.0 884,354 - +1.1 884,354 - -0.4 884,369 - +1.8 884,389 + -0.8 884,389 + -0.4 884,389 + -1.6 884,389 + -1.2 884,389 + -0.4 884,389 + +0.1 884,397 - +1.1 884,398 + -0.9 884,398 + -0.3 884,399 + -0.5 884,399 + -1.1 884,408 + +2.3 884,416 - -0.5 884,421 - +1.0 884,435 - -0.2 884,455 - -2.5 884,463 + -2.5 884,518 - -0.1 884,539 + CCNA_00823 0.12 +0.6 884,562 - -3.1 884,576 - +0.8 884,590 - -1.8 884,666 + -2.8 884,669 - +1.1 884,669 - -1.5 884,738 + -0.1 884,789 + -2.5 884,814 + -4.0 884,821 + -1.5 884,847 - -1.5 884,848 - +0.4 884,877 + -1.4 884,906 + -1.4 884,907 - -2.8 884,932 + -1.2 884,938 - -2.5 884,938 - -2.7 884,940 - -4.2 884,953 + -1.6 884,964 + -0.2 884,964 + +0.4 884,977 - -0.3 885,008 + -2.8 885,016 - +0.8 885,016 - -2.9 885,078 + +0.2 885,086 - -2.0 885,099 + +0.1 885,107 - -2.2 885,113 - -1.2 885,115 + -2.3 885,115 + -2.3 885,115 + -0.9 885,123 - -1.3 885,123 - -1.3 885,196 + -0.4 885,203 - +0.1 885,214 + -2.2 885,223 + -2.9 885,227 + -2.7 885,264 + -2.4 885,274 - +1.4 885,325 + +0.1 885,463 - CCNA_00824 0.19 +1.7 885,482 - CCNA_00824 0.21 +1.1 885,486 + CCNA_00824 0.22 +0.2 885,494 - CCNA_00824 0.23 +0.4 885,534 + CCNA_00824 0.29 -0.1 885,534 + CCNA_00824 0.29 -0.4 885,542 - CCNA_00824 0.30 +0.7 885,542 - CCNA_00824 0.30 +1.3 885,542 - CCNA_00824 0.30 +0.1 885,542 - CCNA_00824 0.30 +0.2 885,542 - CCNA_00824 0.30 +0.9 885,542 - CCNA_00824 0.30 +0.6 885,542 - CCNA_00824 0.30 +1.0 885,543 + CCNA_00824 0.30 +0.4 885,567 + CCNA_00824 0.33 +1.6 885,664 - CCNA_00824 0.46 -0.5 885,664 - CCNA_00824 0.46 +1.3 885,694 - CCNA_00824 0.50 +2.7 885,792 + CCNA_00824 0.64 +2.2 885,795 - CCNA_00824 0.64 +1.0 885,795 - CCNA_00824 0.64 +0.5 885,795 - CCNA_00824 0.64 -0.3 885,813 - CCNA_00824 0.67 +0.5 885,818 - CCNA_00824 0.67 +0.8 885,819 + CCNA_00824 0.68 -2.1 885,831 + CCNA_00824 0.69 -1.0 885,831 + CCNA_00824 0.69 -1.7 885,839 - CCNA_00824 0.70 +1.3 885,849 + CCNA_00824 0.72 +0.6 885,852 - CCNA_00824 0.72 +2.0 885,862 - CCNA_00824 0.74 +0.7 885,867 + CCNA_00824 0.74 -0.5 885,867 + CCNA_00824 0.74 +0.6 885,880 - CCNA_00824 0.76 +1.4 885,880 - CCNA_00824 0.76 +0.6 885,927 + CCNA_00824 0.82 +4.0 885,937 + CCNA_00824 0.84 -0.3 885,944 - CCNA_00824 0.85 +2.2 885,945 - CCNA_00824 0.85 -1.2 885,946 + CCNA_00824 0.85 +0.4 886,018 + -1.4 886,018 + -1.6 886,018 + +0.1 886,018 + -1.6 886,019 + -0.5 886,024 + -0.2 886,034 - +1.3 886,038 - +0.8 886,049 - -0.1 886,092 - +0.3 886,099 + -0.7 886,099 + -1.0 886,107 - -1.0 886,107 - +0.4 886,109 + -0.0 886,113 + -0.3 886,118 + -1.1 886,121 - +0.4 886,146 + -0.3 886,146 + +0.0 886,146 + +0.2 886,148 + -0.9 886,154 - -1.2 886,154 - +1.4 886,154 - -1.8 886,154 - +1.0 886,156 - +0.2 886,294 - CCNA_00825 0.13 +1.4 886,336 + CCNA_00825 0.20 -0.8 886,355 + CCNA_00825 0.23 -0.7 886,359 - CCNA_00825 0.23 +0.9 886,368 + CCNA_00825 0.25 -0.3 886,373 + CCNA_00825 0.26 -0.5 886,376 - CCNA_00825 0.26 -0.4 886,388 + CCNA_00825 0.28 -2.7 886,388 + CCNA_00825 0.28 -1.1 886,398 + CCNA_00825 0.30 +0.8 886,406 - CCNA_00825 0.31 -0.6 886,407 + CCNA_00825 0.31 +3.1 886,407 + CCNA_00825 0.31 +3.1 886,407 + CCNA_00825 0.31 +3.4 886,407 + CCNA_00825 0.31 +3.1 886,412 + CCNA_00825 0.32 +0.5 886,415 - CCNA_00825 0.33 -0.8 886,415 - CCNA_00825 0.33 -0.2 886,447 + CCNA_00825 0.38 -0.4 886,484 + CCNA_00825 0.44 -0.4 886,540 + CCNA_00825 0.53 +0.2 886,568 - CCNA_00825 0.58 -2.3 886,569 - CCNA_00825 0.58 -0.0 886,619 + CCNA_00825 0.66 +0.6 886,619 + CCNA_00825 0.66 +0.3 886,624 + CCNA_00825 0.67 +0.8 886,627 - CCNA_00825 0.67 +1.3 886,627 - CCNA_00825 0.67 -1.6 886,627 - CCNA_00825 0.67 +1.0 886,627 - CCNA_00825 0.67 -0.6 886,643 - CCNA_00825 0.70 -1.6 886,643 - CCNA_00825 0.70 +1.4 886,652 + CCNA_00825 0.71 -0.4 886,660 - CCNA_00825 0.73 -0.5 886,662 - CCNA_00825 0.73 -2.1 886,670 - CCNA_00825 0.74 -1.3 886,687 + CCNA_00825 0.77 -2.3 886,693 + CCNA_00825 0.78 +0.8 886,693 + CCNA_00825 0.78 -1.2 886,701 - CCNA_00825 0.79 +0.7 886,703 + CCNA_00825 0.80 -1.9 886,706 - CCNA_00825 0.80 +0.3 886,711 - CCNA_00825 0.81 -1.0 886,711 - CCNA_00825 0.81 -0.6 886,734 + CCNA_00825 0.85 +0.6 886,734 + CCNA_00825 0.85 +0.4 886,737 - CCNA_00825 0.85 +0.6 886,739 + CCNA_00825 0.86 -0.7 886,793 + -1.7 886,803 - +1.0 886,805 + -0.6 886,805 + -0.7 886,808 - -0.3 886,813 - -1.6 886,813 - +0.6 886,813 - +0.2 886,834 + +1.6 886,847 - -0.8 886,877 + +2.6 886,921 + CCNA_00826 0.11 +1.1 886,929 - CCNA_00826 0.12 +1.0 886,929 - CCNA_00826 0.12 +0.2 886,929 - CCNA_00826 0.12 +0.7 886,947 + CCNA_00826 0.14 -0.4 886,964 + +2.0 886,970 + CCNA_00826 0.16 -2.0 886,974 + CCNA_00826 0.17 -1.5 886,980 + CCNA_00826 0.17 +0.4 886,980 + CCNA_00826 0.17 -0.5 886,980 - CCNA_00826 0.17 -1.8 886,982 - CCNA_00826 0.17 -0.3 886,985 + CCNA_00826 0.18 -0.6 886,985 + CCNA_00826 0.18 -1.1 886,985 + CCNA_00826 0.18 -0.2 886,985 + CCNA_00826 0.18 -1.1 886,985 + CCNA_00826 0.18 -1.4 886,993 - CCNA_00826 0.19 -1.4 886,993 - CCNA_00826 0.19 -0.7 886,993 - CCNA_00826 0.19 -1.2 886,993 - CCNA_00826 0.19 -0.4 887,007 + CCNA_00826 0.20 -1.2 887,015 - CCNA_00826 0.21 -0.1 887,047 - CCNA_00826 0.24 -2.6
Or see this region's nucleotide sequence