Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15515 and BBR_RS20915 are separated by 37 nucleotides BBR_RS20915 and BBR_RS15520 overlap by 12 nucleotides BBR_RS15520 and BBR_RS15525 overlap by 1 nucleotides
BBR_RS15515: BBR_RS15515 - excinuclease ABC subunit UvrC, at 1,253,518 to 1,255,935
_RS15515
BBR_RS20915: BBR_RS20915 - hypothetical protein, at 1,255,973 to 1,256,182
_RS20915
BBR_RS15520: BBR_RS15520 - shikimate 5-dehydrogenase, at 1,256,171 to 1,257,151
_RS15520
BBR_RS15525: BBR_RS15525 - RNase adapter RapZ, at 1,257,151 to 1,258,116
_RS15525
Position (kb)
1255
1256
1257 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1255.068 kb on + strand, within BBR_RS15515 at 1255.090 kb on + strand, within BBR_RS15515 at 1255.091 kb on - strand, within BBR_RS15515 at 1255.093 kb on - strand, within BBR_RS15515 at 1255.093 kb on - strand, within BBR_RS15515 at 1255.097 kb on + strand, within BBR_RS15515 at 1255.104 kb on - strand, within BBR_RS15515 at 1255.151 kb on - strand, within BBR_RS15515 at 1255.151 kb on - strand, within BBR_RS15515 at 1255.305 kb on + strand, within BBR_RS15515 at 1255.340 kb on + strand, within BBR_RS15515 at 1255.348 kb on - strand, within BBR_RS15515 at 1255.457 kb on + strand, within BBR_RS15515 at 1255.457 kb on + strand, within BBR_RS15515 at 1255.465 kb on - strand, within BBR_RS15515 at 1255.468 kb on + strand, within BBR_RS15515 at 1255.542 kb on + strand, within BBR_RS15515 at 1255.545 kb on - strand, within BBR_RS15515 at 1255.561 kb on + strand, within BBR_RS15515 at 1255.581 kb on + strand, within BBR_RS15515 at 1255.630 kb on - strand, within BBR_RS15515 at 1255.656 kb on + strand, within BBR_RS15515 at 1255.675 kb on + strand, within BBR_RS15515 at 1255.675 kb on + strand, within BBR_RS15515 at 1255.676 kb on - strand, within BBR_RS15515 at 1255.677 kb on + strand, within BBR_RS15515 at 1255.795 kb on - strand at 1255.841 kb on - strand at 1255.841 kb on - strand at 1255.847 kb on - strand at 1255.851 kb on + strand at 1255.873 kb on - strand at 1255.873 kb on - strand at 1255.873 kb on - strand at 1255.875 kb on + strand at 1255.875 kb on + strand at 1255.875 kb on + strand at 1255.875 kb on + strand at 1255.875 kb on + strand at 1255.883 kb on - strand at 1255.883 kb on - strand at 1255.893 kb on + strand at 1255.901 kb on - strand at 1255.929 kb on + strand at 1255.937 kb on - strand at 1255.976 kb on + strand at 1255.987 kb on + strand at 1255.994 kb on + strand, within BBR_RS20915 at 1255.995 kb on - strand, within BBR_RS20915 at 1256.033 kb on + strand, within BBR_RS20915 at 1256.041 kb on - strand, within BBR_RS20915 at 1256.064 kb on - strand, within BBR_RS20915 at 1256.120 kb on + strand, within BBR_RS20915 at 1256.128 kb on - strand, within BBR_RS20915 at 1256.128 kb on - strand, within BBR_RS20915 at 1256.307 kb on - strand, within BBR_RS15520 at 1256.317 kb on + strand, within BBR_RS15520 at 1256.506 kb on + strand, within BBR_RS15520 at 1256.512 kb on - strand, within BBR_RS15520 at 1256.546 kb on - strand, within BBR_RS15520 at 1256.597 kb on + strand, within BBR_RS15520 at 1256.742 kb on + strand, within BBR_RS15520 at 1256.796 kb on - strand, within BBR_RS15520 at 1256.813 kb on - strand, within BBR_RS15520 at 1256.945 kb on - strand, within BBR_RS15520 at 1256.965 kb on - strand, within BBR_RS15520 at 1257.028 kb on + strand, within BBR_RS15520 at 1257.136 kb on + strand at 1257.138 kb on + strand at 1257.159 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,255,068 + BBR_RS15515 0.64 +0.0 1,255,090 + BBR_RS15515 0.65 +0.3 1,255,091 - BBR_RS15515 0.65 +1.1 1,255,093 - BBR_RS15515 0.65 +0.5 1,255,093 - BBR_RS15515 0.65 -1.3 1,255,097 + BBR_RS15515 0.65 -0.2 1,255,104 - BBR_RS15515 0.66 -1.2 1,255,151 - BBR_RS15515 0.68 -0.7 1,255,151 - BBR_RS15515 0.68 +0.1 1,255,305 + BBR_RS15515 0.74 -0.3 1,255,340 + BBR_RS15515 0.75 -0.6 1,255,348 - BBR_RS15515 0.76 -0.5 1,255,457 + BBR_RS15515 0.80 +0.2 1,255,457 + BBR_RS15515 0.80 -0.2 1,255,465 - BBR_RS15515 0.81 +0.1 1,255,468 + BBR_RS15515 0.81 +0.3 1,255,542 + BBR_RS15515 0.84 +0.7 1,255,545 - BBR_RS15515 0.84 -0.2 1,255,561 + BBR_RS15515 0.84 -0.5 1,255,581 + BBR_RS15515 0.85 -0.8 1,255,630 - BBR_RS15515 0.87 +0.2 1,255,656 + BBR_RS15515 0.88 -1.2 1,255,675 + BBR_RS15515 0.89 +0.1 1,255,675 + BBR_RS15515 0.89 +1.7 1,255,676 - BBR_RS15515 0.89 -0.3 1,255,677 + BBR_RS15515 0.89 +0.4 1,255,795 - +0.3 1,255,841 - +1.4 1,255,841 - +0.4 1,255,847 - -1.0 1,255,851 + -0.6 1,255,873 - -0.6 1,255,873 - -0.7 1,255,873 - -0.1 1,255,875 + +0.9 1,255,875 + +0.7 1,255,875 + -0.0 1,255,875 + +1.6 1,255,875 + -1.0 1,255,883 - -2.1 1,255,883 - +0.1 1,255,893 + -1.1 1,255,901 - +0.2 1,255,929 + -1.3 1,255,937 - +1.0 1,255,976 + -0.4 1,255,987 + +0.4 1,255,994 + BBR_RS20915 0.10 -0.0 1,255,995 - BBR_RS20915 0.10 -1.9 1,256,033 + BBR_RS20915 0.29 +0.1 1,256,041 - BBR_RS20915 0.32 -0.2 1,256,064 - BBR_RS20915 0.43 +0.5 1,256,120 + BBR_RS20915 0.70 +1.9 1,256,128 - BBR_RS20915 0.74 +0.3 1,256,128 - BBR_RS20915 0.74 +0.6 1,256,307 - BBR_RS15520 0.14 -0.4 1,256,317 + BBR_RS15520 0.15 +0.8 1,256,506 + BBR_RS15520 0.34 -0.1 1,256,512 - BBR_RS15520 0.35 +1.4 1,256,546 - BBR_RS15520 0.38 +2.1 1,256,597 + BBR_RS15520 0.43 -0.2 1,256,742 + BBR_RS15520 0.58 +0.8 1,256,796 - BBR_RS15520 0.64 +0.9 1,256,813 - BBR_RS15520 0.65 -1.0 1,256,945 - BBR_RS15520 0.79 +0.4 1,256,965 - BBR_RS15520 0.81 +1.0 1,257,028 + BBR_RS15520 0.87 +0.0 1,257,136 + +1.8 1,257,138 + +0.4 1,257,159 + -1.0
Or see this region's nucleotide sequence