Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15240 and BBR_RS20890 are separated by 46 nucleotides BBR_RS20890 and BBR_RS20895 are separated by 389 nucleotides BBR_RS20895 and BBR_RS15255 overlap by 36 nucleotides
BBR_RS15240: BBR_RS15240 - LacI family DNA-binding transcriptional regulator, at 1,188,698 to 1,189,726
_RS15240
BBR_RS20890: BBR_RS20890 - hypothetical protein, at 1,189,773 to 1,190,192
_RS20890
BBR_RS20895: BBR_RS20895 - hypothetical protein, at 1,190,582 to 1,190,821
_RS20895
BBR_RS15255: BBR_RS15255 - glutamate synthase large subunit, at 1,190,786 to 1,195,357
_RS15255
Position (kb)
1189
1190
1191 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1188.797 kb on + strand at 1188.799 kb on - strand at 1188.807 kb on - strand, within BBR_RS15240 at 1188.853 kb on - strand, within BBR_RS15240 at 1188.992 kb on - strand, within BBR_RS15240 at 1189.048 kb on + strand, within BBR_RS15240 at 1189.048 kb on + strand, within BBR_RS15240 at 1189.077 kb on + strand, within BBR_RS15240 at 1189.077 kb on + strand, within BBR_RS15240 at 1189.092 kb on + strand, within BBR_RS15240 at 1189.127 kb on + strand, within BBR_RS15240 at 1189.210 kb on - strand, within BBR_RS15240 at 1189.212 kb on - strand, within BBR_RS15240 at 1189.231 kb on + strand, within BBR_RS15240 at 1189.289 kb on + strand, within BBR_RS15240 at 1189.289 kb on + strand, within BBR_RS15240 at 1189.292 kb on - strand, within BBR_RS15240 at 1189.294 kb on - strand, within BBR_RS15240 at 1189.328 kb on - strand, within BBR_RS15240 at 1189.351 kb on + strand, within BBR_RS15240 at 1189.378 kb on + strand, within BBR_RS15240 at 1189.553 kb on + strand, within BBR_RS15240 at 1189.580 kb on - strand, within BBR_RS15240 at 1189.580 kb on - strand, within BBR_RS15240 at 1189.598 kb on + strand, within BBR_RS15240 at 1189.638 kb on - strand at 1189.657 kb on - strand at 1189.687 kb on + strand at 1189.698 kb on + strand at 1189.720 kb on + strand at 1189.729 kb on + strand at 1189.729 kb on + strand at 1189.737 kb on - strand at 1189.737 kb on - strand at 1189.738 kb on - strand at 1189.762 kb on + strand at 1189.778 kb on - strand at 1189.778 kb on - strand at 1189.810 kb on + strand at 1189.831 kb on + strand, within BBR_RS20890 at 1189.831 kb on + strand, within BBR_RS20890 at 1189.831 kb on + strand, within BBR_RS20890 at 1189.831 kb on + strand, within BBR_RS20890 at 1189.839 kb on - strand, within BBR_RS20890 at 1189.839 kb on - strand, within BBR_RS20890 at 1189.839 kb on - strand, within BBR_RS20890 at 1189.884 kb on + strand, within BBR_RS20890 at 1189.935 kb on - strand, within BBR_RS20890 at 1189.975 kb on + strand, within BBR_RS20890 at 1190.093 kb on + strand, within BBR_RS20890 at 1190.126 kb on + strand, within BBR_RS20890 at 1190.138 kb on + strand, within BBR_RS20890 at 1190.138 kb on + strand, within BBR_RS20890 at 1190.145 kb on - strand, within BBR_RS20890 at 1190.173 kb on - strand at 1190.240 kb on + strand at 1190.279 kb on + strand at 1190.280 kb on + strand at 1190.303 kb on - strand at 1190.322 kb on + strand at 1190.330 kb on - strand at 1190.345 kb on + strand at 1190.353 kb on - strand at 1190.433 kb on - strand at 1190.464 kb on - strand at 1190.474 kb on - strand at 1190.489 kb on - strand at 1190.547 kb on - strand at 1190.549 kb on + strand at 1190.722 kb on - strand, within BBR_RS20895 at 1190.791 kb on + strand at 1190.834 kb on + strand at 1190.834 kb on + strand at 1190.836 kb on + strand at 1190.840 kb on - strand at 1190.844 kb on + strand at 1190.860 kb on + strand at 1190.870 kb on - strand at 1190.893 kb on - strand at 1190.938 kb on + strand at 1190.951 kb on - strand at 1190.953 kb on + strand at 1190.975 kb on - strand at 1191.002 kb on + strand at 1191.020 kb on - strand at 1191.035 kb on + strand at 1191.060 kb on - strand at 1191.163 kb on - strand at 1191.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,188,797 + -0.7 1,188,799 - -0.3 1,188,807 - BBR_RS15240 0.11 +1.7 1,188,853 - BBR_RS15240 0.15 +2.9 1,188,992 - BBR_RS15240 0.29 +0.1 1,189,048 + BBR_RS15240 0.34 +1.0 1,189,048 + BBR_RS15240 0.34 +0.0 1,189,077 + BBR_RS15240 0.37 -2.1 1,189,077 + BBR_RS15240 0.37 +0.9 1,189,092 + BBR_RS15240 0.38 -1.8 1,189,127 + BBR_RS15240 0.42 +0.1 1,189,210 - BBR_RS15240 0.50 +0.7 1,189,212 - BBR_RS15240 0.50 -1.4 1,189,231 + BBR_RS15240 0.52 -0.1 1,189,289 + BBR_RS15240 0.57 +0.2 1,189,289 + BBR_RS15240 0.57 -0.3 1,189,292 - BBR_RS15240 0.58 -1.0 1,189,294 - BBR_RS15240 0.58 -1.0 1,189,328 - BBR_RS15240 0.61 +0.5 1,189,351 + BBR_RS15240 0.63 -0.1 1,189,378 + BBR_RS15240 0.66 +0.3 1,189,553 + BBR_RS15240 0.83 +0.0 1,189,580 - BBR_RS15240 0.86 -0.6 1,189,580 - BBR_RS15240 0.86 -0.0 1,189,598 + BBR_RS15240 0.87 +0.9 1,189,638 - +0.4 1,189,657 - +0.2 1,189,687 + +0.5 1,189,698 + +0.9 1,189,720 + +1.1 1,189,729 + -0.2 1,189,729 + -1.2 1,189,737 - -0.5 1,189,737 - -0.9 1,189,738 - -0.8 1,189,762 + +0.3 1,189,778 - -0.8 1,189,778 - -0.3 1,189,810 + +0.3 1,189,831 + BBR_RS20890 0.14 -0.1 1,189,831 + BBR_RS20890 0.14 +1.3 1,189,831 + BBR_RS20890 0.14 -0.5 1,189,831 + BBR_RS20890 0.14 +0.7 1,189,839 - BBR_RS20890 0.16 -0.3 1,189,839 - BBR_RS20890 0.16 +1.5 1,189,839 - BBR_RS20890 0.16 -0.1 1,189,884 + BBR_RS20890 0.26 +0.5 1,189,935 - BBR_RS20890 0.39 -0.1 1,189,975 + BBR_RS20890 0.48 +1.1 1,190,093 + BBR_RS20890 0.76 +0.5 1,190,126 + BBR_RS20890 0.84 -0.0 1,190,138 + BBR_RS20890 0.87 +0.1 1,190,138 + BBR_RS20890 0.87 +0.5 1,190,145 - BBR_RS20890 0.89 +0.0 1,190,173 - -1.5 1,190,240 + -1.4 1,190,279 + +2.9 1,190,280 + +0.6 1,190,303 - +0.3 1,190,322 + +0.3 1,190,330 - +0.7 1,190,345 + -0.6 1,190,353 - -1.4 1,190,433 - +1.2 1,190,464 - -1.1 1,190,474 - +0.4 1,190,489 - +0.3 1,190,547 - -2.1 1,190,549 + -0.3 1,190,722 - BBR_RS20895 0.58 -1.1 1,190,791 + -0.1 1,190,834 + -0.3 1,190,834 + -2.3 1,190,836 + +0.2 1,190,840 - -0.5 1,190,844 + -0.8 1,190,860 + +0.1 1,190,870 - -0.0 1,190,893 - +1.3 1,190,938 + -0.8 1,190,951 - -1.3 1,190,953 + +0.3 1,190,975 - +2.4 1,191,002 + +0.2 1,191,020 - -0.2 1,191,035 + -0.1 1,191,060 - -0.5 1,191,163 - -0.9 1,191,172 - -1.3
Or see this region's nucleotide sequence