Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17575 and BBR_RS17580 are separated by 51 nucleotides BBR_RS17580 and BBR_RS17585 are separated by 50 nucleotides BBR_RS17585 and BBR_RS17590 are separated by 43 nucleotides BBR_RS17590 and BBR_RS17595 overlap by 4 nucleotides
BBR_RS17575: BBR_RS17575 - PTS fructose-like enzyme IIC component, at 1,721,364 to 1,722,482
_RS17575
BBR_RS17580: BBR_RS17580 - PTS fructose transporter subunit IIB, at 1,722,534 to 1,722,845
_RS17580
BBR_RS17585: BBR_RS17585 - PTS fructose transporter subunit IIA, at 1,722,896 to 1,723,366
_RS17585
BBR_RS17590: BBR_RS17590 - PTS sugar transporter subunit IIA, at 1,723,410 to 1,723,838
_RS17590
BBR_RS17595: BBR_RS17595 - transcription antiterminator, at 1,723,835 to 1,725,328
_RS17595
Position (kb)
1722
1723 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1721.535 kb on + strand, within BBR_RS17575 at 1721.535 kb on + strand, within BBR_RS17575 at 1721.586 kb on + strand, within BBR_RS17575 at 1721.588 kb on - strand, within BBR_RS17575 at 1721.608 kb on - strand, within BBR_RS17575 at 1721.608 kb on - strand, within BBR_RS17575 at 1721.666 kb on - strand, within BBR_RS17575 at 1721.684 kb on + strand, within BBR_RS17575 at 1721.689 kb on + strand, within BBR_RS17575 at 1721.689 kb on + strand, within BBR_RS17575 at 1721.697 kb on - strand, within BBR_RS17575 at 1721.716 kb on - strand, within BBR_RS17575 at 1721.735 kb on + strand, within BBR_RS17575 at 1721.735 kb on + strand, within BBR_RS17575 at 1721.743 kb on + strand, within BBR_RS17575 at 1721.743 kb on + strand, within BBR_RS17575 at 1721.750 kb on - strand, within BBR_RS17575 at 1721.751 kb on - strand, within BBR_RS17575 at 1721.795 kb on - strand, within BBR_RS17575 at 1721.820 kb on + strand, within BBR_RS17575 at 1721.830 kb on - strand, within BBR_RS17575 at 1721.849 kb on - strand, within BBR_RS17575 at 1721.856 kb on - strand, within BBR_RS17575 at 1721.861 kb on + strand, within BBR_RS17575 at 1721.861 kb on + strand, within BBR_RS17575 at 1721.897 kb on - strand, within BBR_RS17575 at 1721.921 kb on + strand, within BBR_RS17575 at 1721.921 kb on + strand, within BBR_RS17575 at 1721.921 kb on + strand, within BBR_RS17575 at 1721.929 kb on - strand, within BBR_RS17575 at 1721.935 kb on + strand, within BBR_RS17575 at 1721.962 kb on + strand, within BBR_RS17575 at 1721.970 kb on - strand, within BBR_RS17575 at 1721.970 kb on - strand, within BBR_RS17575 at 1721.972 kb on + strand, within BBR_RS17575 at 1721.972 kb on - strand, within BBR_RS17575 at 1721.980 kb on - strand, within BBR_RS17575 at 1722.029 kb on - strand, within BBR_RS17575 at 1722.045 kb on + strand, within BBR_RS17575 at 1722.045 kb on + strand, within BBR_RS17575 at 1722.046 kb on - strand, within BBR_RS17575 at 1722.077 kb on - strand, within BBR_RS17575 at 1722.087 kb on - strand, within BBR_RS17575 at 1722.107 kb on - strand, within BBR_RS17575 at 1722.134 kb on - strand, within BBR_RS17575 at 1722.193 kb on - strand, within BBR_RS17575 at 1722.224 kb on - strand, within BBR_RS17575 at 1722.254 kb on - strand, within BBR_RS17575 at 1722.280 kb on + strand, within BBR_RS17575 at 1722.325 kb on + strand, within BBR_RS17575 at 1722.364 kb on - strand, within BBR_RS17575 at 1722.374 kb on - strand at 1722.375 kb on - strand at 1722.380 kb on - strand at 1722.390 kb on + strand at 1722.418 kb on - strand at 1722.428 kb on + strand at 1722.573 kb on + strand, within BBR_RS17580 at 1722.677 kb on - strand, within BBR_RS17580 at 1722.685 kb on + strand, within BBR_RS17580 at 1722.690 kb on + strand, within BBR_RS17580 at 1722.693 kb on - strand, within BBR_RS17580 at 1722.739 kb on + strand, within BBR_RS17580 at 1722.741 kb on - strand, within BBR_RS17580 at 1722.746 kb on + strand, within BBR_RS17580 at 1722.747 kb on - strand, within BBR_RS17580 at 1722.747 kb on - strand, within BBR_RS17580 at 1722.754 kb on + strand, within BBR_RS17580 at 1722.754 kb on + strand, within BBR_RS17580 at 1722.788 kb on + strand, within BBR_RS17580 at 1722.793 kb on + strand, within BBR_RS17580 at 1722.801 kb on - strand, within BBR_RS17580 at 1722.801 kb on - strand, within BBR_RS17580 at 1722.806 kb on - strand, within BBR_RS17580 at 1722.828 kb on + strand at 1722.847 kb on + strand at 1722.899 kb on + strand at 1722.908 kb on - strand at 1722.936 kb on + strand at 1722.936 kb on + strand at 1722.936 kb on + strand at 1722.936 kb on + strand at 1722.944 kb on - strand, within BBR_RS17585 at 1723.142 kb on - strand, within BBR_RS17585 at 1723.145 kb on + strand, within BBR_RS17585 at 1723.145 kb on + strand, within BBR_RS17585 at 1723.153 kb on - strand, within BBR_RS17585 at 1723.191 kb on + strand, within BBR_RS17585 at 1723.197 kb on + strand, within BBR_RS17585 at 1723.205 kb on - strand, within BBR_RS17585 at 1723.205 kb on - strand, within BBR_RS17585 at 1723.207 kb on - strand, within BBR_RS17585 at 1723.210 kb on - strand, within BBR_RS17585 at 1723.217 kb on - strand, within BBR_RS17585 at 1723.298 kb on - strand, within BBR_RS17585 at 1723.298 kb on - strand, within BBR_RS17585 at 1723.304 kb on - strand, within BBR_RS17585 at 1723.304 kb on - strand, within BBR_RS17585 at 1723.320 kb on - strand at 1723.322 kb on - strand at 1723.336 kb on + strand at 1723.342 kb on + strand at 1723.342 kb on + strand at 1723.348 kb on + strand at 1723.350 kb on - strand at 1723.350 kb on - strand at 1723.356 kb on - strand at 1723.356 kb on - strand at 1723.356 kb on - strand at 1723.374 kb on - strand at 1723.376 kb on - strand at 1723.447 kb on + strand at 1723.447 kb on + strand at 1723.527 kb on - strand, within BBR_RS17590 at 1723.527 kb on - strand, within BBR_RS17590 at 1723.527 kb on - strand, within BBR_RS17590 at 1723.537 kb on - strand, within BBR_RS17590 at 1723.637 kb on + strand, within BBR_RS17590 at 1723.637 kb on + strand, within BBR_RS17590 at 1723.645 kb on - strand, within BBR_RS17590 at 1723.683 kb on + strand, within BBR_RS17590 at 1723.708 kb on + strand, within BBR_RS17590 at 1723.754 kb on + strand, within BBR_RS17590 at 1723.762 kb on - strand, within BBR_RS17590 at 1723.776 kb on + strand, within BBR_RS17590 at 1723.792 kb on - strand, within BBR_RS17590 at 1723.817 kb on + strand at 1723.827 kb on - strand at 1723.831 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,721,535 + BBR_RS17575 0.15 -0.1 1,721,535 + BBR_RS17575 0.15 -0.2 1,721,586 + BBR_RS17575 0.20 -0.1 1,721,588 - BBR_RS17575 0.20 +0.6 1,721,608 - BBR_RS17575 0.22 +0.1 1,721,608 - BBR_RS17575 0.22 -0.9 1,721,666 - BBR_RS17575 0.27 +1.1 1,721,684 + BBR_RS17575 0.29 -0.1 1,721,689 + BBR_RS17575 0.29 -0.5 1,721,689 + BBR_RS17575 0.29 +0.6 1,721,697 - BBR_RS17575 0.30 +0.7 1,721,716 - BBR_RS17575 0.31 -1.2 1,721,735 + BBR_RS17575 0.33 +0.2 1,721,735 + BBR_RS17575 0.33 +0.3 1,721,743 + BBR_RS17575 0.34 +0.2 1,721,743 + BBR_RS17575 0.34 +0.3 1,721,750 - BBR_RS17575 0.34 +0.3 1,721,751 - BBR_RS17575 0.35 +0.0 1,721,795 - BBR_RS17575 0.39 +0.1 1,721,820 + BBR_RS17575 0.41 +0.7 1,721,830 - BBR_RS17575 0.42 +0.0 1,721,849 - BBR_RS17575 0.43 +0.4 1,721,856 - BBR_RS17575 0.44 -2.3 1,721,861 + BBR_RS17575 0.44 -0.0 1,721,861 + BBR_RS17575 0.44 +0.6 1,721,897 - BBR_RS17575 0.48 +0.3 1,721,921 + BBR_RS17575 0.50 -0.0 1,721,921 + BBR_RS17575 0.50 -0.3 1,721,921 + BBR_RS17575 0.50 -0.3 1,721,929 - BBR_RS17575 0.50 +0.8 1,721,935 + BBR_RS17575 0.51 -0.5 1,721,962 + BBR_RS17575 0.53 -0.8 1,721,970 - BBR_RS17575 0.54 +0.0 1,721,970 - BBR_RS17575 0.54 +0.5 1,721,972 + BBR_RS17575 0.54 +0.1 1,721,972 - BBR_RS17575 0.54 -0.4 1,721,980 - BBR_RS17575 0.55 -0.3 1,722,029 - BBR_RS17575 0.59 +0.1 1,722,045 + BBR_RS17575 0.61 -1.3 1,722,045 + BBR_RS17575 0.61 -0.2 1,722,046 - BBR_RS17575 0.61 +0.2 1,722,077 - BBR_RS17575 0.64 +0.5 1,722,087 - BBR_RS17575 0.65 +0.3 1,722,107 - BBR_RS17575 0.66 -0.2 1,722,134 - BBR_RS17575 0.69 -0.3 1,722,193 - BBR_RS17575 0.74 +3.4 1,722,224 - BBR_RS17575 0.77 +0.0 1,722,254 - BBR_RS17575 0.80 -2.1 1,722,280 + BBR_RS17575 0.82 +1.0 1,722,325 + BBR_RS17575 0.86 -0.8 1,722,364 - BBR_RS17575 0.89 +0.5 1,722,374 - +0.4 1,722,375 - -1.8 1,722,380 - +0.6 1,722,390 + +0.7 1,722,418 - +0.4 1,722,428 + -0.4 1,722,573 + BBR_RS17580 0.12 -1.9 1,722,677 - BBR_RS17580 0.46 -0.2 1,722,685 + BBR_RS17580 0.48 +2.6 1,722,690 + BBR_RS17580 0.50 +0.0 1,722,693 - BBR_RS17580 0.51 +0.3 1,722,739 + BBR_RS17580 0.66 +0.5 1,722,741 - BBR_RS17580 0.66 +0.4 1,722,746 + BBR_RS17580 0.68 +0.7 1,722,747 - BBR_RS17580 0.68 +0.8 1,722,747 - BBR_RS17580 0.68 -0.5 1,722,754 + BBR_RS17580 0.71 +0.3 1,722,754 + BBR_RS17580 0.71 +1.5 1,722,788 + BBR_RS17580 0.81 +0.5 1,722,793 + BBR_RS17580 0.83 -0.1 1,722,801 - BBR_RS17580 0.86 -0.3 1,722,801 - BBR_RS17580 0.86 +0.5 1,722,806 - BBR_RS17580 0.87 -0.0 1,722,828 + +0.0 1,722,847 + +0.6 1,722,899 + +0.8 1,722,908 - +0.8 1,722,936 + -1.2 1,722,936 + -0.5 1,722,936 + +0.2 1,722,936 + +0.2 1,722,944 - BBR_RS17585 0.10 +0.1 1,723,142 - BBR_RS17585 0.52 +0.1 1,723,145 + BBR_RS17585 0.53 -1.6 1,723,145 + BBR_RS17585 0.53 -0.3 1,723,153 - BBR_RS17585 0.55 +0.3 1,723,191 + BBR_RS17585 0.63 -0.9 1,723,197 + BBR_RS17585 0.64 +1.7 1,723,205 - BBR_RS17585 0.66 +0.5 1,723,205 - BBR_RS17585 0.66 +0.8 1,723,207 - BBR_RS17585 0.66 -0.1 1,723,210 - BBR_RS17585 0.67 -0.2 1,723,217 - BBR_RS17585 0.68 +0.3 1,723,298 - BBR_RS17585 0.85 -0.2 1,723,298 - BBR_RS17585 0.85 -0.1 1,723,304 - BBR_RS17585 0.87 +0.1 1,723,304 - BBR_RS17585 0.87 +0.5 1,723,320 - +0.1 1,723,322 - +0.1 1,723,336 + +0.1 1,723,342 + -0.5 1,723,342 + +0.3 1,723,348 + -0.2 1,723,350 - -0.4 1,723,350 - -0.0 1,723,356 - +0.5 1,723,356 - -0.6 1,723,356 - -0.6 1,723,374 - -0.4 1,723,376 - +0.1 1,723,447 + +0.6 1,723,447 + -0.6 1,723,527 - BBR_RS17590 0.27 +0.5 1,723,527 - BBR_RS17590 0.27 -0.1 1,723,527 - BBR_RS17590 0.27 -1.6 1,723,537 - BBR_RS17590 0.30 -0.3 1,723,637 + BBR_RS17590 0.53 +0.0 1,723,637 + BBR_RS17590 0.53 +0.7 1,723,645 - BBR_RS17590 0.55 -0.1 1,723,683 + BBR_RS17590 0.64 -0.4 1,723,708 + BBR_RS17590 0.69 -0.2 1,723,754 + BBR_RS17590 0.80 -1.1 1,723,762 - BBR_RS17590 0.82 -0.4 1,723,776 + BBR_RS17590 0.85 -1.7 1,723,792 - BBR_RS17590 0.89 -1.8 1,723,817 + +0.4 1,723,827 - -0.1 1,723,831 - +0.7
Or see this region's nucleotide sequence