Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17245 and BBR_RS17250 are separated by 43 nucleotides BBR_RS17250 and BBR_RS17255 are separated by 153 nucleotides BBR_RS17255 and BBR_RS17260 are separated by 46 nucleotides
BBR_RS17245: BBR_RS17245 - serpin family protein, at 1,647,336 to 1,648,769
_RS17245
BBR_RS17250: BBR_RS17250 - LacI family DNA-binding transcriptional regulator, at 1,648,813 to 1,649,853
_RS17250
BBR_RS17255: BBR_RS17255 - ClbS/DfsB family four-helix bundle protein, at 1,650,007 to 1,650,525
_RS17255
BBR_RS17260: BBR_RS17260 - Sucrose permease, at 1,650,572 to 1,651,042
_RS17260
Position (kb)
1648
1649
1650 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1647.923 kb on - strand, within BBR_RS17245 at 1647.969 kb on + strand, within BBR_RS17245 at 1647.989 kb on + strand, within BBR_RS17245 at 1648.009 kb on + strand, within BBR_RS17245 at 1648.012 kb on + strand, within BBR_RS17245 at 1648.017 kb on - strand, within BBR_RS17245 at 1648.025 kb on - strand, within BBR_RS17245 at 1648.025 kb on - strand, within BBR_RS17245 at 1648.028 kb on + strand, within BBR_RS17245 at 1648.057 kb on + strand, within BBR_RS17245 at 1648.065 kb on - strand, within BBR_RS17245 at 1648.074 kb on + strand, within BBR_RS17245 at 1648.145 kb on - strand, within BBR_RS17245 at 1648.165 kb on + strand, within BBR_RS17245 at 1648.175 kb on + strand, within BBR_RS17245 at 1648.181 kb on - strand, within BBR_RS17245 at 1648.183 kb on - strand, within BBR_RS17245 at 1648.183 kb on - strand, within BBR_RS17245 at 1648.192 kb on + strand, within BBR_RS17245 at 1648.192 kb on + strand, within BBR_RS17245 at 1648.214 kb on + strand, within BBR_RS17245 at 1648.249 kb on - strand, within BBR_RS17245 at 1648.249 kb on - strand, within BBR_RS17245 at 1648.265 kb on + strand, within BBR_RS17245 at 1648.326 kb on - strand, within BBR_RS17245 at 1648.338 kb on + strand, within BBR_RS17245 at 1648.345 kb on - strand, within BBR_RS17245 at 1648.352 kb on + strand, within BBR_RS17245 at 1648.352 kb on - strand, within BBR_RS17245 at 1648.360 kb on - strand, within BBR_RS17245 at 1648.375 kb on - strand, within BBR_RS17245 at 1648.421 kb on + strand, within BBR_RS17245 at 1648.424 kb on - strand, within BBR_RS17245 at 1648.473 kb on - strand, within BBR_RS17245 at 1648.484 kb on + strand, within BBR_RS17245 at 1648.489 kb on + strand, within BBR_RS17245 at 1648.489 kb on + strand, within BBR_RS17245 at 1648.515 kb on - strand, within BBR_RS17245 at 1648.517 kb on + strand, within BBR_RS17245 at 1648.525 kb on - strand, within BBR_RS17245 at 1648.571 kb on - strand, within BBR_RS17245 at 1648.573 kb on + strand, within BBR_RS17245 at 1648.590 kb on - strand, within BBR_RS17245 at 1648.590 kb on - strand, within BBR_RS17245 at 1648.605 kb on + strand, within BBR_RS17245 at 1648.605 kb on + strand, within BBR_RS17245 at 1648.605 kb on + strand, within BBR_RS17245 at 1648.612 kb on - strand, within BBR_RS17245 at 1648.612 kb on - strand, within BBR_RS17245 at 1648.613 kb on - strand, within BBR_RS17245 at 1648.613 kb on - strand, within BBR_RS17245 at 1648.632 kb on + strand at 1648.632 kb on + strand at 1648.634 kb on + strand at 1648.634 kb on + strand at 1648.640 kb on - strand at 1648.642 kb on - strand at 1648.650 kb on + strand at 1648.650 kb on + strand at 1648.655 kb on - strand at 1648.683 kb on + strand at 1648.719 kb on - strand at 1648.729 kb on + strand at 1648.744 kb on - strand at 1648.772 kb on - strand at 1648.789 kb on + strand at 1648.789 kb on + strand at 1648.809 kb on + strand at 1648.841 kb on + strand at 1648.841 kb on + strand at 1648.841 kb on + strand at 1648.867 kb on - strand at 1648.867 kb on - strand at 1648.878 kb on - strand at 1648.881 kb on - strand at 1648.881 kb on - strand at 1648.903 kb on - strand at 1648.932 kb on - strand, within BBR_RS17250 at 1648.971 kb on - strand, within BBR_RS17250 at 1648.987 kb on + strand, within BBR_RS17250 at 1648.987 kb on + strand, within BBR_RS17250 at 1648.990 kb on - strand, within BBR_RS17250 at 1648.995 kb on - strand, within BBR_RS17250 at 1649.015 kb on + strand, within BBR_RS17250 at 1649.025 kb on + strand, within BBR_RS17250 at 1649.027 kb on + strand, within BBR_RS17250 at 1649.035 kb on - strand, within BBR_RS17250 at 1649.072 kb on + strand, within BBR_RS17250 at 1649.087 kb on + strand, within BBR_RS17250 at 1649.095 kb on - strand, within BBR_RS17250 at 1649.100 kb on - strand, within BBR_RS17250 at 1649.109 kb on - strand, within BBR_RS17250 at 1649.116 kb on + strand, within BBR_RS17250 at 1649.198 kb on + strand, within BBR_RS17250 at 1649.218 kb on - strand, within BBR_RS17250 at 1649.223 kb on + strand, within BBR_RS17250 at 1649.223 kb on + strand, within BBR_RS17250 at 1649.238 kb on + strand, within BBR_RS17250 at 1649.241 kb on - strand, within BBR_RS17250 at 1649.336 kb on - strand, within BBR_RS17250 at 1649.353 kb on + strand, within BBR_RS17250 at 1649.354 kb on + strand, within BBR_RS17250 at 1649.361 kb on + strand, within BBR_RS17250 at 1649.366 kb on + strand, within BBR_RS17250 at 1649.366 kb on + strand, within BBR_RS17250 at 1649.366 kb on + strand, within BBR_RS17250 at 1649.396 kb on - strand, within BBR_RS17250 at 1649.412 kb on - strand, within BBR_RS17250 at 1649.450 kb on + strand, within BBR_RS17250 at 1649.454 kb on + strand, within BBR_RS17250 at 1649.466 kb on + strand, within BBR_RS17250 at 1649.477 kb on + strand, within BBR_RS17250 at 1649.482 kb on + strand, within BBR_RS17250 at 1649.555 kb on + strand, within BBR_RS17250 at 1649.563 kb on + strand, within BBR_RS17250 at 1649.604 kb on + strand, within BBR_RS17250 at 1649.604 kb on + strand, within BBR_RS17250 at 1649.604 kb on + strand, within BBR_RS17250 at 1649.604 kb on + strand, within BBR_RS17250 at 1649.672 kb on + strand, within BBR_RS17250 at 1649.672 kb on + strand, within BBR_RS17250 at 1649.677 kb on - strand, within BBR_RS17250 at 1649.680 kb on - strand, within BBR_RS17250 at 1649.700 kb on + strand, within BBR_RS17250 at 1649.707 kb on + strand, within BBR_RS17250 at 1649.708 kb on - strand, within BBR_RS17250 at 1649.763 kb on + strand at 1649.825 kb on - strand at 1649.853 kb on + strand at 1649.935 kb on + strand at 1650.000 kb on - strand at 1650.032 kb on + strand at 1650.048 kb on - strand at 1650.098 kb on + strand, within BBR_RS17255 at 1650.098 kb on + strand, within BBR_RS17255 at 1650.123 kb on + strand, within BBR_RS17255 at 1650.124 kb on + strand, within BBR_RS17255 at 1650.132 kb on - strand, within BBR_RS17255 at 1650.181 kb on - strand, within BBR_RS17255 at 1650.217 kb on - strand, within BBR_RS17255 at 1650.217 kb on - strand, within BBR_RS17255 at 1650.237 kb on + strand, within BBR_RS17255 at 1650.237 kb on + strand, within BBR_RS17255 at 1650.262 kb on + strand, within BBR_RS17255 at 1650.264 kb on + strand, within BBR_RS17255 at 1650.285 kb on - strand, within BBR_RS17255 at 1650.373 kb on + strand, within BBR_RS17255 at 1650.379 kb on - strand, within BBR_RS17255 at 1650.381 kb on + strand, within BBR_RS17255 at 1650.381 kb on - strand, within BBR_RS17255 at 1650.389 kb on - strand, within BBR_RS17255 at 1650.429 kb on + strand, within BBR_RS17255 at 1650.439 kb on + strand, within BBR_RS17255 at 1650.444 kb on - strand, within BBR_RS17255 at 1650.446 kb on + strand, within BBR_RS17255 at 1650.447 kb on - strand, within BBR_RS17255 at 1650.457 kb on - strand, within BBR_RS17255 at 1650.464 kb on + strand, within BBR_RS17255 at 1650.474 kb on + strand at 1650.517 kb on + strand at 1650.517 kb on + strand at 1650.525 kb on - strand at 1650.526 kb on + strand at 1650.595 kb on + strand at 1650.595 kb on - strand at 1650.629 kb on - strand, within BBR_RS17260 at 1650.648 kb on - strand, within BBR_RS17260 at 1650.661 kb on + strand, within BBR_RS17260 at 1650.669 kb on - strand, within BBR_RS17260 at 1650.671 kb on + strand, within BBR_RS17260 at 1650.755 kb on - strand, within BBR_RS17260 at 1650.757 kb on - strand, within BBR_RS17260 at 1650.833 kb on - strand, within BBR_RS17260 at 1650.839 kb on + strand, within BBR_RS17260 at 1650.852 kb on - strand, within BBR_RS17260 at 1650.852 kb on - strand, within BBR_RS17260
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,647,923 - BBR_RS17245 0.41 -0.1 1,647,969 + BBR_RS17245 0.44 +0.3 1,647,989 + BBR_RS17245 0.46 +0.6 1,648,009 + BBR_RS17245 0.47 +0.6 1,648,012 + BBR_RS17245 0.47 -0.4 1,648,017 - BBR_RS17245 0.47 +0.4 1,648,025 - BBR_RS17245 0.48 -0.4 1,648,025 - BBR_RS17245 0.48 +0.3 1,648,028 + BBR_RS17245 0.48 +0.2 1,648,057 + BBR_RS17245 0.50 -0.3 1,648,065 - BBR_RS17245 0.51 +0.3 1,648,074 + BBR_RS17245 0.51 -0.5 1,648,145 - BBR_RS17245 0.56 -1.4 1,648,165 + BBR_RS17245 0.58 -0.2 1,648,175 + BBR_RS17245 0.59 -0.1 1,648,181 - BBR_RS17245 0.59 -0.5 1,648,183 - BBR_RS17245 0.59 -0.1 1,648,183 - BBR_RS17245 0.59 +0.8 1,648,192 + BBR_RS17245 0.60 +0.0 1,648,192 + BBR_RS17245 0.60 +0.7 1,648,214 + BBR_RS17245 0.61 -0.6 1,648,249 - BBR_RS17245 0.64 +0.1 1,648,249 - BBR_RS17245 0.64 -0.3 1,648,265 + BBR_RS17245 0.65 +0.5 1,648,326 - BBR_RS17245 0.69 -1.4 1,648,338 + BBR_RS17245 0.70 -0.1 1,648,345 - BBR_RS17245 0.70 -0.0 1,648,352 + BBR_RS17245 0.71 +0.1 1,648,352 - BBR_RS17245 0.71 -0.1 1,648,360 - BBR_RS17245 0.71 -1.1 1,648,375 - BBR_RS17245 0.72 +0.9 1,648,421 + BBR_RS17245 0.76 +0.1 1,648,424 - BBR_RS17245 0.76 +1.1 1,648,473 - BBR_RS17245 0.79 -0.2 1,648,484 + BBR_RS17245 0.80 +0.3 1,648,489 + BBR_RS17245 0.80 -0.2 1,648,489 + BBR_RS17245 0.80 +0.2 1,648,515 - BBR_RS17245 0.82 -0.4 1,648,517 + BBR_RS17245 0.82 +0.2 1,648,525 - BBR_RS17245 0.83 +0.4 1,648,571 - BBR_RS17245 0.86 +0.4 1,648,573 + BBR_RS17245 0.86 +0.6 1,648,590 - BBR_RS17245 0.87 +0.1 1,648,590 - BBR_RS17245 0.87 +0.8 1,648,605 + BBR_RS17245 0.88 -0.8 1,648,605 + BBR_RS17245 0.88 +0.1 1,648,605 + BBR_RS17245 0.88 -0.4 1,648,612 - BBR_RS17245 0.89 -0.2 1,648,612 - BBR_RS17245 0.89 +1.0 1,648,613 - BBR_RS17245 0.89 -0.8 1,648,613 - BBR_RS17245 0.89 +0.5 1,648,632 + -0.8 1,648,632 + +1.4 1,648,634 + +1.8 1,648,634 + +0.9 1,648,640 - -0.7 1,648,642 - -0.7 1,648,650 + +1.2 1,648,650 + +1.3 1,648,655 - +0.6 1,648,683 + +0.8 1,648,719 - -1.0 1,648,729 + -0.1 1,648,744 - +0.4 1,648,772 - -0.2 1,648,789 + -1.9 1,648,789 + -0.2 1,648,809 + +0.8 1,648,841 + +0.3 1,648,841 + +0.3 1,648,841 + +0.4 1,648,867 - +0.6 1,648,867 - -0.3 1,648,878 - +0.0 1,648,881 - -0.1 1,648,881 - +0.0 1,648,903 - +0.2 1,648,932 - BBR_RS17250 0.11 +0.4 1,648,971 - BBR_RS17250 0.15 +0.3 1,648,987 + BBR_RS17250 0.17 +0.6 1,648,987 + BBR_RS17250 0.17 +1.0 1,648,990 - BBR_RS17250 0.17 -0.4 1,648,995 - BBR_RS17250 0.17 +0.1 1,649,015 + BBR_RS17250 0.19 -0.0 1,649,025 + BBR_RS17250 0.20 +0.2 1,649,027 + BBR_RS17250 0.21 +1.1 1,649,035 - BBR_RS17250 0.21 +1.2 1,649,072 + BBR_RS17250 0.25 +0.8 1,649,087 + BBR_RS17250 0.26 -1.1 1,649,095 - BBR_RS17250 0.27 +0.3 1,649,100 - BBR_RS17250 0.28 +0.2 1,649,109 - BBR_RS17250 0.28 -0.1 1,649,116 + BBR_RS17250 0.29 +0.2 1,649,198 + BBR_RS17250 0.37 -0.2 1,649,218 - BBR_RS17250 0.39 -1.1 1,649,223 + BBR_RS17250 0.39 +0.2 1,649,223 + BBR_RS17250 0.39 +0.0 1,649,238 + BBR_RS17250 0.41 -1.3 1,649,241 - BBR_RS17250 0.41 +0.8 1,649,336 - BBR_RS17250 0.50 +0.5 1,649,353 + BBR_RS17250 0.52 +1.3 1,649,354 + BBR_RS17250 0.52 -0.2 1,649,361 + BBR_RS17250 0.53 +0.3 1,649,366 + BBR_RS17250 0.53 -0.1 1,649,366 + BBR_RS17250 0.53 +0.0 1,649,366 + BBR_RS17250 0.53 +1.4 1,649,396 - BBR_RS17250 0.56 +0.3 1,649,412 - BBR_RS17250 0.58 -1.2 1,649,450 + BBR_RS17250 0.61 +0.3 1,649,454 + BBR_RS17250 0.62 -0.9 1,649,466 + BBR_RS17250 0.63 -0.4 1,649,477 + BBR_RS17250 0.64 +1.1 1,649,482 + BBR_RS17250 0.64 +0.3 1,649,555 + BBR_RS17250 0.71 +0.0 1,649,563 + BBR_RS17250 0.72 +0.9 1,649,604 + BBR_RS17250 0.76 -0.1 1,649,604 + BBR_RS17250 0.76 +0.0 1,649,604 + BBR_RS17250 0.76 +1.1 1,649,604 + BBR_RS17250 0.76 -1.2 1,649,672 + BBR_RS17250 0.83 +1.0 1,649,672 + BBR_RS17250 0.83 -0.2 1,649,677 - BBR_RS17250 0.83 +0.1 1,649,680 - BBR_RS17250 0.83 -0.4 1,649,700 + BBR_RS17250 0.85 -3.0 1,649,707 + BBR_RS17250 0.86 +0.8 1,649,708 - BBR_RS17250 0.86 -0.0 1,649,763 + +0.6 1,649,825 - +1.0 1,649,853 + -0.0 1,649,935 + -0.2 1,650,000 - +0.4 1,650,032 + -0.2 1,650,048 - +0.2 1,650,098 + BBR_RS17255 0.18 +0.0 1,650,098 + BBR_RS17255 0.18 +0.3 1,650,123 + BBR_RS17255 0.22 -1.4 1,650,124 + BBR_RS17255 0.23 -0.2 1,650,132 - BBR_RS17255 0.24 +1.2 1,650,181 - BBR_RS17255 0.34 +0.2 1,650,217 - BBR_RS17255 0.40 -0.3 1,650,217 - BBR_RS17255 0.40 -0.1 1,650,237 + BBR_RS17255 0.44 +0.1 1,650,237 + BBR_RS17255 0.44 +0.0 1,650,262 + BBR_RS17255 0.49 +0.6 1,650,264 + BBR_RS17255 0.50 +0.4 1,650,285 - BBR_RS17255 0.54 +0.2 1,650,373 + BBR_RS17255 0.71 +0.0 1,650,379 - BBR_RS17255 0.72 -0.7 1,650,381 + BBR_RS17255 0.72 +0.0 1,650,381 - BBR_RS17255 0.72 -0.1 1,650,389 - BBR_RS17255 0.74 +0.1 1,650,429 + BBR_RS17255 0.81 -1.0 1,650,439 + BBR_RS17255 0.83 -0.6 1,650,444 - BBR_RS17255 0.84 +0.4 1,650,446 + BBR_RS17255 0.85 +0.1 1,650,447 - BBR_RS17255 0.85 +1.1 1,650,457 - BBR_RS17255 0.87 +0.9 1,650,464 + BBR_RS17255 0.88 +0.4 1,650,474 + +0.6 1,650,517 + -0.8 1,650,517 + +0.5 1,650,525 - -0.1 1,650,526 + -1.1 1,650,595 + +1.4 1,650,595 - -0.9 1,650,629 - BBR_RS17260 0.12 -0.3 1,650,648 - BBR_RS17260 0.16 -1.1 1,650,661 + BBR_RS17260 0.19 +0.9 1,650,669 - BBR_RS17260 0.21 +0.4 1,650,671 + BBR_RS17260 0.21 -0.1 1,650,755 - BBR_RS17260 0.39 -0.7 1,650,757 - BBR_RS17260 0.39 -1.5 1,650,833 - BBR_RS17260 0.55 +2.2 1,650,839 + BBR_RS17260 0.57 +0.0 1,650,852 - BBR_RS17260 0.59 -0.3 1,650,852 - BBR_RS17260 0.59 -0.7
Or see this region's nucleotide sequence