Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16570 and BBR_RS16575 are separated by 132 nucleotides BBR_RS16575 and BBR_RS16580 overlap by 4 nucleotides
BBR_RS16570: BBR_RS16570 - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 1,484,492 to 1,485,943
_RS16570
BBR_RS16575: BBR_RS16575 - (p)ppGpp synthetase, at 1,486,076 to 1,486,816
_RS16575
BBR_RS16580: BBR_RS16580 - EamA/RhaT family transporter, at 1,486,813 to 1,487,760
_RS16580
Position (kb)
1486
1487 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1485.241 kb on - strand, within BBR_RS16570 at 1485.276 kb on - strand, within BBR_RS16570 at 1485.330 kb on - strand, within BBR_RS16570 at 1485.365 kb on - strand, within BBR_RS16570 at 1485.366 kb on + strand, within BBR_RS16570 at 1485.369 kb on - strand, within BBR_RS16570 at 1485.458 kb on - strand, within BBR_RS16570 at 1485.460 kb on + strand, within BBR_RS16570 at 1485.469 kb on + strand, within BBR_RS16570 at 1485.477 kb on + strand, within BBR_RS16570 at 1485.477 kb on + strand, within BBR_RS16570 at 1485.485 kb on - strand, within BBR_RS16570 at 1485.485 kb on - strand, within BBR_RS16570 at 1485.490 kb on - strand, within BBR_RS16570 at 1485.498 kb on - strand, within BBR_RS16570 at 1485.571 kb on - strand, within BBR_RS16570 at 1485.637 kb on - strand, within BBR_RS16570 at 1485.658 kb on - strand, within BBR_RS16570 at 1485.709 kb on + strand, within BBR_RS16570 at 1485.709 kb on + strand, within BBR_RS16570 at 1485.717 kb on - strand, within BBR_RS16570 at 1485.722 kb on + strand, within BBR_RS16570 at 1485.725 kb on - strand, within BBR_RS16570 at 1485.784 kb on - strand, within BBR_RS16570 at 1485.879 kb on + strand at 1485.879 kb on + strand at 1485.887 kb on - strand at 1485.916 kb on + strand at 1485.996 kb on - strand at 1486.059 kb on + strand at 1486.116 kb on + strand at 1486.116 kb on + strand at 1486.124 kb on + strand at 1486.165 kb on + strand, within BBR_RS16575 at 1486.165 kb on + strand, within BBR_RS16575 at 1486.348 kb on + strand, within BBR_RS16575 at 1486.400 kb on - strand, within BBR_RS16575 at 1486.425 kb on - strand, within BBR_RS16575 at 1486.438 kb on + strand, within BBR_RS16575 at 1486.438 kb on + strand, within BBR_RS16575 at 1486.438 kb on + strand, within BBR_RS16575 at 1486.446 kb on - strand, within BBR_RS16575 at 1486.446 kb on - strand, within BBR_RS16575 at 1486.446 kb on - strand, within BBR_RS16575 at 1486.578 kb on - strand, within BBR_RS16575 at 1486.578 kb on - strand, within BBR_RS16575 at 1486.606 kb on + strand, within BBR_RS16575 at 1486.647 kb on - strand, within BBR_RS16575 at 1486.662 kb on + strand, within BBR_RS16575 at 1486.714 kb on - strand, within BBR_RS16575 at 1486.716 kb on - strand, within BBR_RS16575 at 1486.735 kb on - strand, within BBR_RS16575 at 1486.813 kb on - strand at 1486.879 kb on - strand at 1486.888 kb on + strand at 1486.888 kb on + strand at 1486.894 kb on - strand at 1486.904 kb on - strand at 1486.907 kb on + strand at 1486.907 kb on + strand at 1486.912 kb on + strand, within BBR_RS16580 at 1486.915 kb on - strand, within BBR_RS16580 at 1486.934 kb on + strand, within BBR_RS16580 at 1487.002 kb on - strand, within BBR_RS16580 at 1487.002 kb on - strand, within BBR_RS16580 at 1487.007 kb on - strand, within BBR_RS16580 at 1487.021 kb on - strand, within BBR_RS16580 at 1487.058 kb on - strand, within BBR_RS16580 at 1487.066 kb on + strand, within BBR_RS16580 at 1487.138 kb on + strand, within BBR_RS16580 at 1487.141 kb on + strand, within BBR_RS16580 at 1487.141 kb on + strand, within BBR_RS16580 at 1487.146 kb on + strand, within BBR_RS16580 at 1487.178 kb on + strand, within BBR_RS16580 at 1487.216 kb on + strand, within BBR_RS16580 at 1487.229 kb on - strand, within BBR_RS16580 at 1487.229 kb on - strand, within BBR_RS16580 at 1487.431 kb on - strand, within BBR_RS16580 at 1487.536 kb on + strand, within BBR_RS16580 at 1487.536 kb on + strand, within BBR_RS16580 at 1487.539 kb on - strand, within BBR_RS16580 at 1487.544 kb on - strand, within BBR_RS16580 at 1487.544 kb on - strand, within BBR_RS16580 at 1487.602 kb on + strand, within BBR_RS16580 at 1487.674 kb on - strand at 1487.684 kb on - strand at 1487.693 kb on + strand at 1487.693 kb on + strand at 1487.699 kb on + strand at 1487.726 kb on + strand at 1487.796 kb on - strand at 1487.809 kb on - strand at 1487.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,485,241 - BBR_RS16570 0.52 +0.6 1,485,276 - BBR_RS16570 0.54 +0.1 1,485,330 - BBR_RS16570 0.58 +0.8 1,485,365 - BBR_RS16570 0.60 +0.4 1,485,366 + BBR_RS16570 0.60 +0.3 1,485,369 - BBR_RS16570 0.60 -0.0 1,485,458 - BBR_RS16570 0.67 +0.9 1,485,460 + BBR_RS16570 0.67 -0.2 1,485,469 + BBR_RS16570 0.67 +0.7 1,485,477 + BBR_RS16570 0.68 +0.6 1,485,477 + BBR_RS16570 0.68 +1.0 1,485,485 - BBR_RS16570 0.68 -0.0 1,485,485 - BBR_RS16570 0.68 -0.2 1,485,490 - BBR_RS16570 0.69 +0.5 1,485,498 - BBR_RS16570 0.69 +0.1 1,485,571 - BBR_RS16570 0.74 +1.1 1,485,637 - BBR_RS16570 0.79 -0.7 1,485,658 - BBR_RS16570 0.80 +0.3 1,485,709 + BBR_RS16570 0.84 +1.1 1,485,709 + BBR_RS16570 0.84 +0.4 1,485,717 - BBR_RS16570 0.84 -0.6 1,485,722 + BBR_RS16570 0.85 -1.0 1,485,725 - BBR_RS16570 0.85 +0.6 1,485,784 - BBR_RS16570 0.89 +0.6 1,485,879 + +0.5 1,485,879 + +0.0 1,485,887 - +2.4 1,485,916 + +2.4 1,485,996 - -0.1 1,486,059 + -2.5 1,486,116 + -0.6 1,486,116 + -0.1 1,486,124 + +0.0 1,486,165 + BBR_RS16575 0.12 +1.1 1,486,165 + BBR_RS16575 0.12 -0.1 1,486,348 + BBR_RS16575 0.37 -0.0 1,486,400 - BBR_RS16575 0.44 -0.7 1,486,425 - BBR_RS16575 0.47 +0.5 1,486,438 + BBR_RS16575 0.49 +1.2 1,486,438 + BBR_RS16575 0.49 -0.5 1,486,438 + BBR_RS16575 0.49 +0.5 1,486,446 - BBR_RS16575 0.50 +0.7 1,486,446 - BBR_RS16575 0.50 +0.0 1,486,446 - BBR_RS16575 0.50 +0.3 1,486,578 - BBR_RS16575 0.68 +0.1 1,486,578 - BBR_RS16575 0.68 +0.5 1,486,606 + BBR_RS16575 0.72 +0.4 1,486,647 - BBR_RS16575 0.77 -0.1 1,486,662 + BBR_RS16575 0.79 +0.5 1,486,714 - BBR_RS16575 0.86 +0.8 1,486,716 - BBR_RS16575 0.86 +0.3 1,486,735 - BBR_RS16575 0.89 +0.1 1,486,813 - +0.2 1,486,879 - +1.2 1,486,888 + -0.4 1,486,888 + +1.5 1,486,894 - -0.0 1,486,904 - -1.8 1,486,907 + -1.7 1,486,907 + +0.6 1,486,912 + BBR_RS16580 0.10 +0.1 1,486,915 - BBR_RS16580 0.11 +1.1 1,486,934 + BBR_RS16580 0.13 -0.7 1,487,002 - BBR_RS16580 0.20 -0.1 1,487,002 - BBR_RS16580 0.20 -0.9 1,487,007 - BBR_RS16580 0.20 -0.7 1,487,021 - BBR_RS16580 0.22 -0.3 1,487,058 - BBR_RS16580 0.26 +3.4 1,487,066 + BBR_RS16580 0.27 +0.1 1,487,138 + BBR_RS16580 0.34 -0.3 1,487,141 + BBR_RS16580 0.35 +0.0 1,487,141 + BBR_RS16580 0.35 +0.2 1,487,146 + BBR_RS16580 0.35 +0.4 1,487,178 + BBR_RS16580 0.39 -0.8 1,487,216 + BBR_RS16580 0.43 -1.3 1,487,229 - BBR_RS16580 0.44 +0.5 1,487,229 - BBR_RS16580 0.44 -0.3 1,487,431 - BBR_RS16580 0.65 +1.4 1,487,536 + BBR_RS16580 0.76 -0.1 1,487,536 + BBR_RS16580 0.76 -0.3 1,487,539 - BBR_RS16580 0.77 +1.2 1,487,544 - BBR_RS16580 0.77 -0.2 1,487,544 - BBR_RS16580 0.77 -0.0 1,487,602 + BBR_RS16580 0.83 +0.5 1,487,674 - -0.1 1,487,684 - +1.2 1,487,693 + -0.6 1,487,693 + -0.1 1,487,699 + +0.1 1,487,726 + +0.4 1,487,796 - -0.2 1,487,809 - +0.4 1,487,809 - -0.6
Or see this region's nucleotide sequence