Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15455 and BBR_RS15460 are separated by 107 nucleotides BBR_RS15460 and BBR_RS15465 are separated by 367 nucleotides BBR_RS15465 and BBR_RS15470 are separated by 156 nucleotides
BBR_RS15455: BBR_RS15455 - orotate phosphoribosyltransferase, at 1,238,579 to 1,239,274
_RS15455
BBR_RS15460: BBR_RS15460 - hypothetical protein, at 1,239,382 to 1,239,570
_RS15460
BBR_RS15465: BBR_RS15465 - DUF2974 domain-containing protein, at 1,239,938 to 1,241,059
_RS15465
BBR_RS15470: BBR_RS15470 - LysR family transcriptional regulator, at 1,241,216 to 1,242,139
_RS15470
Position (kb)
1239
1240
1241
1242 Strain fitness (log2 ratio)
-1
0
1
2 at 1238.941 kb on + strand, within BBR_RS15455 at 1239.030 kb on - strand, within BBR_RS15455 at 1239.384 kb on + strand at 1239.398 kb on + strand at 1239.398 kb on + strand at 1239.398 kb on + strand at 1239.404 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.485 kb on - strand, within BBR_RS15460 at 1239.560 kb on - strand at 1239.662 kb on - strand at 1239.677 kb on + strand at 1239.773 kb on - strand at 1239.846 kb on + strand at 1239.846 kb on + strand at 1239.846 kb on + strand at 1239.854 kb on - strand at 1239.929 kb on - strand at 1239.933 kb on + strand at 1239.991 kb on + strand at 1240.006 kb on + strand at 1240.104 kb on - strand, within BBR_RS15465 at 1240.113 kb on - strand, within BBR_RS15465 at 1240.160 kb on + strand, within BBR_RS15465 at 1240.284 kb on - strand, within BBR_RS15465 at 1240.315 kb on + strand, within BBR_RS15465 at 1240.323 kb on - strand, within BBR_RS15465 at 1240.446 kb on - strand, within BBR_RS15465 at 1240.602 kb on + strand, within BBR_RS15465 at 1240.687 kb on + strand, within BBR_RS15465 at 1240.697 kb on + strand, within BBR_RS15465 at 1240.697 kb on - strand, within BBR_RS15465 at 1240.699 kb on + strand, within BBR_RS15465 at 1240.705 kb on + strand, within BBR_RS15465 at 1240.707 kb on - strand, within BBR_RS15465 at 1240.768 kb on + strand, within BBR_RS15465 at 1240.783 kb on + strand, within BBR_RS15465 at 1240.792 kb on - strand, within BBR_RS15465 at 1240.971 kb on + strand at 1241.014 kb on - strand at 1241.016 kb on - strand at 1241.017 kb on - strand at 1241.056 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.153 kb on + strand at 1241.209 kb on - strand at 1241.221 kb on - strand at 1241.261 kb on - strand at 1241.283 kb on + strand at 1241.291 kb on - strand at 1241.335 kb on + strand, within BBR_RS15470 at 1241.355 kb on + strand, within BBR_RS15470 at 1241.404 kb on + strand, within BBR_RS15470 at 1241.406 kb on + strand, within BBR_RS15470 at 1241.412 kb on - strand, within BBR_RS15470 at 1241.412 kb on - strand, within BBR_RS15470 at 1241.440 kb on - strand, within BBR_RS15470 at 1241.472 kb on + strand, within BBR_RS15470 at 1241.480 kb on - strand, within BBR_RS15470 at 1241.497 kb on - strand, within BBR_RS15470 at 1241.520 kb on + strand, within BBR_RS15470 at 1241.603 kb on - strand, within BBR_RS15470 at 1241.654 kb on - strand, within BBR_RS15470 at 1241.693 kb on - strand, within BBR_RS15470 at 1241.711 kb on - strand, within BBR_RS15470 at 1241.712 kb on - strand, within BBR_RS15470 at 1241.799 kb on - strand, within BBR_RS15470 at 1241.849 kb on - strand, within BBR_RS15470 at 1241.872 kb on - strand, within BBR_RS15470 at 1241.888 kb on - strand, within BBR_RS15470 at 1242.013 kb on + strand, within BBR_RS15470 at 1242.013 kb on + strand, within BBR_RS15470 at 1242.041 kb on + strand, within BBR_RS15470 at 1242.041 kb on + strand, within BBR_RS15470 at 1242.052 kb on + strand at 1242.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,238,941 + BBR_RS15455 0.52 -0.7 1,239,030 - BBR_RS15455 0.65 +0.3 1,239,384 + +0.3 1,239,398 + -0.5 1,239,398 + +0.2 1,239,398 + +0.2 1,239,404 - BBR_RS15460 0.12 +0.9 1,239,406 - BBR_RS15460 0.13 -1.2 1,239,406 - BBR_RS15460 0.13 -0.4 1,239,406 - BBR_RS15460 0.13 +0.4 1,239,485 - BBR_RS15460 0.54 -0.5 1,239,560 - -0.8 1,239,662 - +0.7 1,239,677 + +0.7 1,239,773 - -0.2 1,239,846 + +0.0 1,239,846 + -0.2 1,239,846 + -0.6 1,239,854 - +1.9 1,239,929 - -0.4 1,239,933 + -1.1 1,239,991 + +0.5 1,240,006 + +0.7 1,240,104 - BBR_RS15465 0.15 +0.1 1,240,113 - BBR_RS15465 0.16 +0.8 1,240,160 + BBR_RS15465 0.20 -0.2 1,240,284 - BBR_RS15465 0.31 +0.3 1,240,315 + BBR_RS15465 0.34 +1.1 1,240,323 - BBR_RS15465 0.34 +0.3 1,240,446 - BBR_RS15465 0.45 +1.0 1,240,602 + BBR_RS15465 0.59 -0.7 1,240,687 + BBR_RS15465 0.67 -0.2 1,240,697 + BBR_RS15465 0.68 -0.4 1,240,697 - BBR_RS15465 0.68 -0.2 1,240,699 + BBR_RS15465 0.68 +0.9 1,240,705 + BBR_RS15465 0.68 -0.2 1,240,707 - BBR_RS15465 0.69 -0.1 1,240,768 + BBR_RS15465 0.74 +1.0 1,240,783 + BBR_RS15465 0.75 +1.3 1,240,792 - BBR_RS15465 0.76 -0.7 1,240,971 + +2.7 1,241,014 - +1.0 1,241,016 - +0.2 1,241,017 - -0.2 1,241,056 + +0.5 1,241,090 + -1.6 1,241,090 + +0.2 1,241,153 + -0.7 1,241,209 - +0.1 1,241,221 - +0.4 1,241,261 - +0.1 1,241,283 + +2.0 1,241,291 - -1.0 1,241,335 + BBR_RS15470 0.13 -0.3 1,241,355 + BBR_RS15470 0.15 -0.1 1,241,404 + BBR_RS15470 0.20 -0.4 1,241,406 + BBR_RS15470 0.21 +0.1 1,241,412 - BBR_RS15470 0.21 +0.6 1,241,412 - BBR_RS15470 0.21 -0.1 1,241,440 - BBR_RS15470 0.24 -1.4 1,241,472 + BBR_RS15470 0.28 +0.3 1,241,480 - BBR_RS15470 0.29 -0.0 1,241,497 - BBR_RS15470 0.30 +0.2 1,241,520 + BBR_RS15470 0.33 -0.1 1,241,603 - BBR_RS15470 0.42 +0.9 1,241,654 - BBR_RS15470 0.47 -1.5 1,241,693 - BBR_RS15470 0.52 -0.7 1,241,711 - BBR_RS15470 0.54 +0.3 1,241,712 - BBR_RS15470 0.54 -0.1 1,241,799 - BBR_RS15470 0.63 +0.3 1,241,849 - BBR_RS15470 0.69 -0.5 1,241,872 - BBR_RS15470 0.71 +0.5 1,241,888 - BBR_RS15470 0.73 -0.8 1,242,013 + BBR_RS15470 0.86 -0.4 1,242,013 + BBR_RS15470 0.86 -0.1 1,242,041 + BBR_RS15470 0.89 -0.8 1,242,041 + BBR_RS15470 0.89 +2.3 1,242,052 + -1.1 1,242,054 - -1.7
Or see this region's nucleotide sequence