Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14835 and BBR_RS14840 are separated by 112 nucleotides BBR_RS14840 and BBR_RS14845 are separated by 5 nucleotides BBR_RS14845 and BBR_RS14850 are separated by 139 nucleotides BBR_RS14850 and BBR_RS20870 are separated by 132 nucleotides BBR_RS20870 and BBR_RS14860 are separated by 148 nucleotides
BBR_RS14835: BBR_RS14835 - sulfite exporter TauE/SafE family protein, at 1,104,200 to 1,105,000
_RS14835
BBR_RS14840: BBR_RS14840 - IS3 family transposase, at 1,105,113 to 1,105,436
_RS14840
BBR_RS14845: BBR_RS14845 - permease, at 1,105,442 to 1,106,051
_RS14845
BBR_RS14850: BBR_RS14850 - VOC family protein, at 1,106,191 to 1,106,553
_RS14850
BBR_RS20870: BBR_RS20870 - hypothetical protein, at 1,106,686 to 1,106,865
_RS20870
BBR_RS14860: BBR_RS14860 - MFS transporter, at 1,107,014 to 1,108,384
_RS14860
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-2
-1
0
1 at 1104.442 kb on - strand, within BBR_RS14835 at 1104.447 kb on - strand, within BBR_RS14835 at 1104.450 kb on + strand, within BBR_RS14835 at 1104.470 kb on - strand, within BBR_RS14835 at 1104.470 kb on - strand, within BBR_RS14835 at 1104.485 kb on + strand, within BBR_RS14835 at 1104.513 kb on - strand, within BBR_RS14835 at 1104.588 kb on - strand, within BBR_RS14835 at 1104.593 kb on - strand, within BBR_RS14835 at 1104.608 kb on + strand, within BBR_RS14835 at 1104.643 kb on + strand, within BBR_RS14835 at 1104.781 kb on + strand, within BBR_RS14835 at 1104.813 kb on - strand, within BBR_RS14835 at 1104.834 kb on - strand, within BBR_RS14835 at 1104.839 kb on - strand, within BBR_RS14835 at 1104.866 kb on + strand, within BBR_RS14835 at 1104.976 kb on + strand at 1105.028 kb on - strand at 1105.090 kb on + strand at 1105.098 kb on - strand at 1105.170 kb on - strand, within BBR_RS14840 at 1105.172 kb on + strand, within BBR_RS14840 at 1105.172 kb on + strand, within BBR_RS14840 at 1105.180 kb on - strand, within BBR_RS14840 at 1105.180 kb on - strand, within BBR_RS14840 at 1105.187 kb on + strand, within BBR_RS14840 at 1105.200 kb on - strand, within BBR_RS14840 at 1105.253 kb on + strand, within BBR_RS14840 at 1105.292 kb on + strand, within BBR_RS14840 at 1105.295 kb on - strand, within BBR_RS14840 at 1105.302 kb on + strand, within BBR_RS14840 at 1105.362 kb on - strand, within BBR_RS14840 at 1105.362 kb on - strand, within BBR_RS14840 at 1105.449 kb on + strand at 1105.539 kb on + strand, within BBR_RS14845 at 1105.553 kb on - strand, within BBR_RS14845 at 1105.716 kb on - strand, within BBR_RS14845 at 1105.765 kb on + strand, within BBR_RS14845 at 1105.817 kb on - strand, within BBR_RS14845 at 1105.832 kb on - strand, within BBR_RS14845 at 1105.914 kb on - strand, within BBR_RS14845 at 1105.918 kb on + strand, within BBR_RS14845 at 1105.952 kb on + strand, within BBR_RS14845 at 1106.043 kb on + strand at 1106.046 kb on - strand at 1106.090 kb on - strand at 1106.152 kb on - strand at 1106.177 kb on - strand at 1106.189 kb on + strand at 1106.189 kb on + strand at 1106.194 kb on + strand at 1106.202 kb on - strand at 1106.204 kb on + strand at 1106.243 kb on + strand, within BBR_RS14850 at 1106.391 kb on - strand, within BBR_RS14850 at 1106.489 kb on - strand, within BBR_RS14850 at 1106.529 kb on - strand at 1106.537 kb on - strand at 1106.569 kb on - strand at 1106.685 kb on - strand at 1106.726 kb on + strand, within BBR_RS20870 at 1106.801 kb on + strand, within BBR_RS20870 at 1106.885 kb on + strand at 1106.888 kb on - strand at 1106.933 kb on + strand at 1106.933 kb on + strand at 1106.946 kb on - strand at 1106.968 kb on - strand at 1106.988 kb on + strand at 1107.000 kb on + strand at 1107.010 kb on + strand at 1107.050 kb on - strand at 1107.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,104,442 - BBR_RS14835 0.30 +0.5 1,104,447 - BBR_RS14835 0.31 +0.3 1,104,450 + BBR_RS14835 0.31 -0.1 1,104,470 - BBR_RS14835 0.34 -0.9 1,104,470 - BBR_RS14835 0.34 -0.3 1,104,485 + BBR_RS14835 0.36 +0.5 1,104,513 - BBR_RS14835 0.39 -0.9 1,104,588 - BBR_RS14835 0.48 +1.1 1,104,593 - BBR_RS14835 0.49 +0.6 1,104,608 + BBR_RS14835 0.51 -0.9 1,104,643 + BBR_RS14835 0.55 -0.2 1,104,781 + BBR_RS14835 0.73 -0.2 1,104,813 - BBR_RS14835 0.77 +0.1 1,104,834 - BBR_RS14835 0.79 -0.0 1,104,839 - BBR_RS14835 0.80 +0.8 1,104,866 + BBR_RS14835 0.83 -0.3 1,104,976 + +0.3 1,105,028 - -1.2 1,105,090 + -0.9 1,105,098 - +0.3 1,105,170 - BBR_RS14840 0.18 -1.4 1,105,172 + BBR_RS14840 0.18 +0.1 1,105,172 + BBR_RS14840 0.18 -0.1 1,105,180 - BBR_RS14840 0.21 -0.2 1,105,180 - BBR_RS14840 0.21 -0.8 1,105,187 + BBR_RS14840 0.23 +0.3 1,105,200 - BBR_RS14840 0.27 -0.0 1,105,253 + BBR_RS14840 0.43 +0.0 1,105,292 + BBR_RS14840 0.55 +0.0 1,105,295 - BBR_RS14840 0.56 -2.0 1,105,302 + BBR_RS14840 0.58 -0.8 1,105,362 - BBR_RS14840 0.77 -0.1 1,105,362 - BBR_RS14840 0.77 -1.5 1,105,449 + -1.0 1,105,539 + BBR_RS14845 0.16 +0.6 1,105,553 - BBR_RS14845 0.18 +0.2 1,105,716 - BBR_RS14845 0.45 -1.1 1,105,765 + BBR_RS14845 0.53 +0.5 1,105,817 - BBR_RS14845 0.61 +0.4 1,105,832 - BBR_RS14845 0.64 -0.4 1,105,914 - BBR_RS14845 0.77 +0.0 1,105,918 + BBR_RS14845 0.78 +0.5 1,105,952 + BBR_RS14845 0.84 +0.4 1,106,043 + +0.2 1,106,046 - +0.1 1,106,090 - -0.0 1,106,152 - -0.8 1,106,177 - +0.1 1,106,189 + -0.1 1,106,189 + +0.2 1,106,194 + +0.4 1,106,202 - -0.9 1,106,204 + -0.0 1,106,243 + BBR_RS14850 0.14 +1.1 1,106,391 - BBR_RS14850 0.55 +0.7 1,106,489 - BBR_RS14850 0.82 +0.5 1,106,529 - +0.8 1,106,537 - +0.4 1,106,569 - -0.5 1,106,685 - +1.0 1,106,726 + BBR_RS20870 0.22 -0.2 1,106,801 + BBR_RS20870 0.64 -1.0 1,106,885 + -0.5 1,106,888 - +0.1 1,106,933 + +0.9 1,106,933 + -0.2 1,106,946 - +0.2 1,106,968 - +0.2 1,106,988 + +1.1 1,107,000 + +0.3 1,107,010 + -0.0 1,107,050 - +1.4 1,107,050 - -0.9
Or see this region's nucleotide sequence