Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14520 and BBR_RS14525 are separated by 106 nucleotides BBR_RS14525 and BBR_RS14530 are separated by 29 nucleotides BBR_RS14530 and BBR_RS14535 are separated by 106 nucleotides BBR_RS14535 and BBR_RS14540 are separated by 80 nucleotides
BBR_RS14520: BBR_RS14520 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 1,031,543 to 1,032,904
_RS14520
BBR_RS14525: BBR_RS14525 - tRNA-Arg, at 1,033,011 to 1,033,084
_RS14525
BBR_RS14530: BBR_RS14530 - tRNA-Arg, at 1,033,114 to 1,033,190
_RS14530
BBR_RS14535: BBR_RS14535 - pyridoxal phosphate-dependent aminotransferase, at 1,033,297 to 1,034,502
_RS14535
BBR_RS14540: BBR_RS14540 - Na+/H+ antiporter NhaA, at 1,034,583 to 1,035,971
_RS14540
Position (kb)
1033
1034
1035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1032.323 kb on + strand, within BBR_RS14520 at 1032.335 kb on + strand, within BBR_RS14520 at 1032.418 kb on - strand, within BBR_RS14520 at 1032.523 kb on + strand, within BBR_RS14520 at 1032.619 kb on + strand, within BBR_RS14520 at 1032.623 kb on + strand, within BBR_RS14520 at 1032.786 kb on + strand at 1032.786 kb on + strand at 1032.794 kb on - strand at 1032.833 kb on - strand at 1032.840 kb on - strand at 1032.907 kb on + strand at 1032.933 kb on + strand at 1033.246 kb on + strand at 1033.277 kb on + strand at 1033.393 kb on + strand at 1033.393 kb on + strand at 1033.440 kb on + strand, within BBR_RS14535 at 1033.487 kb on + strand, within BBR_RS14535 at 1033.516 kb on + strand, within BBR_RS14535 at 1033.542 kb on + strand, within BBR_RS14535 at 1033.580 kb on + strand, within BBR_RS14535 at 1033.586 kb on + strand, within BBR_RS14535 at 1033.714 kb on + strand, within BBR_RS14535 at 1033.736 kb on + strand, within BBR_RS14535 at 1033.811 kb on + strand, within BBR_RS14535 at 1033.816 kb on + strand, within BBR_RS14535 at 1033.816 kb on + strand, within BBR_RS14535 at 1033.835 kb on - strand, within BBR_RS14535 at 1033.839 kb on + strand, within BBR_RS14535 at 1033.841 kb on + strand, within BBR_RS14535 at 1033.854 kb on + strand, within BBR_RS14535 at 1033.957 kb on - strand, within BBR_RS14535 at 1033.979 kb on - strand, within BBR_RS14535 at 1034.057 kb on + strand, within BBR_RS14535 at 1034.167 kb on + strand, within BBR_RS14535 at 1034.167 kb on + strand, within BBR_RS14535 at 1034.167 kb on + strand, within BBR_RS14535 at 1034.183 kb on + strand, within BBR_RS14535 at 1034.202 kb on + strand, within BBR_RS14535 at 1034.298 kb on - strand, within BBR_RS14535 at 1034.435 kb on - strand at 1034.473 kb on + strand at 1034.480 kb on - strand at 1034.497 kb on + strand at 1034.502 kb on + strand at 1034.505 kb on - strand at 1034.641 kb on + strand at 1034.804 kb on - strand, within BBR_RS14540 at 1034.861 kb on - strand, within BBR_RS14540 at 1034.898 kb on + strand, within BBR_RS14540 at 1034.961 kb on - strand, within BBR_RS14540 at 1034.977 kb on + strand, within BBR_RS14540 at 1035.119 kb on + strand, within BBR_RS14540 at 1035.140 kb on + strand, within BBR_RS14540 at 1035.221 kb on - strand, within BBR_RS14540 at 1035.393 kb on - strand, within BBR_RS14540 at 1035.443 kb on + strand, within BBR_RS14540 at 1035.462 kb on + strand, within BBR_RS14540 at 1035.486 kb on + strand, within BBR_RS14540
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,032,323 + BBR_RS14520 0.57 +2.3 1,032,335 + BBR_RS14520 0.58 +0.4 1,032,418 - BBR_RS14520 0.64 +0.9 1,032,523 + BBR_RS14520 0.72 +0.5 1,032,619 + BBR_RS14520 0.79 +0.6 1,032,623 + BBR_RS14520 0.79 -0.5 1,032,786 + +1.4 1,032,786 + -0.8 1,032,794 - +0.9 1,032,833 - -0.0 1,032,840 - +0.8 1,032,907 + -2.5 1,032,933 + -0.3 1,033,246 + +0.7 1,033,277 + -1.8 1,033,393 + +0.7 1,033,393 + +0.3 1,033,440 + BBR_RS14535 0.12 -0.0 1,033,487 + BBR_RS14535 0.16 +0.6 1,033,516 + BBR_RS14535 0.18 +0.6 1,033,542 + BBR_RS14535 0.20 +1.6 1,033,580 + BBR_RS14535 0.23 +0.9 1,033,586 + BBR_RS14535 0.24 +0.9 1,033,714 + BBR_RS14535 0.35 +0.7 1,033,736 + BBR_RS14535 0.36 +0.3 1,033,811 + BBR_RS14535 0.43 +0.0 1,033,816 + BBR_RS14535 0.43 +0.7 1,033,816 + BBR_RS14535 0.43 -0.1 1,033,835 - BBR_RS14535 0.45 +2.3 1,033,839 + BBR_RS14535 0.45 -1.9 1,033,841 + BBR_RS14535 0.45 +0.8 1,033,854 + BBR_RS14535 0.46 +0.5 1,033,957 - BBR_RS14535 0.55 +0.1 1,033,979 - BBR_RS14535 0.57 +1.0 1,034,057 + BBR_RS14535 0.63 -1.1 1,034,167 + BBR_RS14535 0.72 -0.6 1,034,167 + BBR_RS14535 0.72 +1.5 1,034,167 + BBR_RS14535 0.72 -0.6 1,034,183 + BBR_RS14535 0.73 -1.8 1,034,202 + BBR_RS14535 0.75 -0.5 1,034,298 - BBR_RS14535 0.83 +0.3 1,034,435 - -0.7 1,034,473 + -1.0 1,034,480 - -0.9 1,034,497 + -0.1 1,034,502 + -0.5 1,034,505 - +0.0 1,034,641 + +2.3 1,034,804 - BBR_RS14540 0.16 -0.5 1,034,861 - BBR_RS14540 0.20 +0.3 1,034,898 + BBR_RS14540 0.23 -1.6 1,034,961 - BBR_RS14540 0.27 -0.5 1,034,977 + BBR_RS14540 0.28 -0.3 1,035,119 + BBR_RS14540 0.39 -2.4 1,035,140 + BBR_RS14540 0.40 +2.3 1,035,221 - BBR_RS14540 0.46 +0.7 1,035,393 - BBR_RS14540 0.58 +0.9 1,035,443 + BBR_RS14540 0.62 -0.3 1,035,462 + BBR_RS14540 0.63 -0.7 1,035,486 + BBR_RS14540 0.65 +0.3
Or see this region's nucleotide sequence