Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14440 and BBR_RS14445 are separated by 248 nucleotides BBR_RS14445 and BBR_RS14450 are separated by 168 nucleotides
BBR_RS14440: BBR_RS14440 - Cof-type HAD-IIB family hydrolase, at 1,013,347 to 1,014,156
_RS14440
BBR_RS14445: BBR_RS14445 - Solute-binding protein of ABC transporter system, at 1,014,405 to 1,015,385
_RS14445
BBR_RS14450: BBR_RS14450 - methionine ABC transporter ATP-binding protein, at 1,015,554 to 1,016,756
_RS14450
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1013.447 kb on + strand, within BBR_RS14440 at 1013.468 kb on + strand, within BBR_RS14440 at 1013.474 kb on + strand, within BBR_RS14440 at 1013.526 kb on + strand, within BBR_RS14440 at 1013.548 kb on - strand, within BBR_RS14440 at 1013.566 kb on + strand, within BBR_RS14440 at 1013.566 kb on + strand, within BBR_RS14440 at 1013.588 kb on + strand, within BBR_RS14440 at 1013.593 kb on + strand, within BBR_RS14440 at 1013.646 kb on + strand, within BBR_RS14440 at 1013.789 kb on - strand, within BBR_RS14440 at 1013.799 kb on + strand, within BBR_RS14440 at 1013.888 kb on - strand, within BBR_RS14440 at 1013.897 kb on - strand, within BBR_RS14440 at 1013.897 kb on - strand, within BBR_RS14440 at 1013.930 kb on - strand, within BBR_RS14440 at 1013.957 kb on - strand, within BBR_RS14440 at 1013.968 kb on + strand, within BBR_RS14440 at 1013.980 kb on + strand, within BBR_RS14440 at 1013.990 kb on + strand, within BBR_RS14440 at 1014.076 kb on + strand at 1014.134 kb on + strand at 1014.194 kb on + strand at 1014.223 kb on + strand at 1014.246 kb on + strand at 1014.252 kb on - strand at 1014.355 kb on + strand at 1014.363 kb on - strand at 1014.412 kb on + strand at 1014.425 kb on - strand at 1014.446 kb on - strand at 1014.456 kb on - strand at 1014.467 kb on + strand at 1014.476 kb on - strand at 1014.485 kb on - strand at 1014.578 kb on + strand, within BBR_RS14445 at 1014.601 kb on + strand, within BBR_RS14445 at 1014.605 kb on - strand, within BBR_RS14445 at 1014.634 kb on + strand, within BBR_RS14445 at 1014.769 kb on - strand, within BBR_RS14445 at 1014.769 kb on - strand, within BBR_RS14445 at 1014.791 kb on + strand, within BBR_RS14445 at 1014.793 kb on + strand, within BBR_RS14445 at 1014.793 kb on + strand, within BBR_RS14445 at 1014.801 kb on + strand, within BBR_RS14445 at 1014.809 kb on - strand, within BBR_RS14445 at 1014.854 kb on + strand, within BBR_RS14445 at 1014.855 kb on + strand, within BBR_RS14445 at 1014.855 kb on - strand, within BBR_RS14445 at 1014.863 kb on + strand, within BBR_RS14445 at 1014.887 kb on + strand, within BBR_RS14445 at 1014.938 kb on + strand, within BBR_RS14445 at 1014.938 kb on + strand, within BBR_RS14445 at 1014.946 kb on - strand, within BBR_RS14445 at 1014.946 kb on - strand, within BBR_RS14445 at 1014.946 kb on - strand, within BBR_RS14445 at 1014.946 kb on - strand, within BBR_RS14445 at 1014.963 kb on + strand, within BBR_RS14445 at 1014.976 kb on + strand, within BBR_RS14445 at 1015.011 kb on + strand, within BBR_RS14445 at 1015.019 kb on - strand, within BBR_RS14445 at 1015.022 kb on + strand, within BBR_RS14445 at 1015.022 kb on + strand, within BBR_RS14445 at 1015.027 kb on + strand, within BBR_RS14445 at 1015.029 kb on - strand, within BBR_RS14445 at 1015.029 kb on - strand, within BBR_RS14445 at 1015.029 kb on - strand, within BBR_RS14445 at 1015.042 kb on + strand, within BBR_RS14445 at 1015.071 kb on + strand, within BBR_RS14445 at 1015.097 kb on - strand, within BBR_RS14445 at 1015.157 kb on + strand, within BBR_RS14445 at 1015.212 kb on + strand, within BBR_RS14445 at 1015.276 kb on - strand, within BBR_RS14445 at 1015.327 kb on - strand at 1015.349 kb on + strand at 1015.453 kb on - strand at 1015.488 kb on - strand at 1015.500 kb on + strand at 1015.565 kb on + strand at 1015.616 kb on + strand at 1015.718 kb on - strand, within BBR_RS14450 at 1015.737 kb on - strand, within BBR_RS14450 at 1015.785 kb on + strand, within BBR_RS14450 at 1015.785 kb on + strand, within BBR_RS14450 at 1015.812 kb on + strand, within BBR_RS14450 at 1015.892 kb on + strand, within BBR_RS14450 at 1015.905 kb on - strand, within BBR_RS14450 at 1015.933 kb on + strand, within BBR_RS14450 at 1015.935 kb on + strand, within BBR_RS14450 at 1015.977 kb on + strand, within BBR_RS14450 at 1015.994 kb on + strand, within BBR_RS14450 at 1015.995 kb on - strand, within BBR_RS14450 at 1015.995 kb on - strand, within BBR_RS14450 at 1015.995 kb on - strand, within BBR_RS14450 at 1015.995 kb on - strand, within BBR_RS14450 at 1015.995 kb on - strand, within BBR_RS14450 at 1015.995 kb on - strand, within BBR_RS14450 at 1016.000 kb on - strand, within BBR_RS14450 at 1016.002 kb on - strand, within BBR_RS14450 at 1016.002 kb on - strand, within BBR_RS14450 at 1016.004 kb on + strand, within BBR_RS14450 at 1016.013 kb on - strand, within BBR_RS14450 at 1016.091 kb on + strand, within BBR_RS14450 at 1016.109 kb on + strand, within BBR_RS14450 at 1016.114 kb on + strand, within BBR_RS14450 at 1016.120 kb on + strand, within BBR_RS14450 at 1016.174 kb on + strand, within BBR_RS14450 at 1016.239 kb on + strand, within BBR_RS14450 at 1016.246 kb on + strand, within BBR_RS14450 at 1016.246 kb on + strand, within BBR_RS14450 at 1016.254 kb on - strand, within BBR_RS14450 at 1016.304 kb on + strand, within BBR_RS14450 at 1016.304 kb on + strand, within BBR_RS14450 at 1016.322 kb on + strand, within BBR_RS14450 at 1016.348 kb on - strand, within BBR_RS14450 at 1016.371 kb on + strand, within BBR_RS14450 at 1016.374 kb on - strand, within BBR_RS14450
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 1,013,447 + BBR_RS14440 0.12 +0.4 1,013,468 + BBR_RS14440 0.15 -0.3 1,013,474 + BBR_RS14440 0.16 +0.9 1,013,526 + BBR_RS14440 0.22 +0.3 1,013,548 - BBR_RS14440 0.25 +0.7 1,013,566 + BBR_RS14440 0.27 +0.1 1,013,566 + BBR_RS14440 0.27 +0.0 1,013,588 + BBR_RS14440 0.30 +0.2 1,013,593 + BBR_RS14440 0.30 +0.3 1,013,646 + BBR_RS14440 0.37 -0.1 1,013,789 - BBR_RS14440 0.55 -0.5 1,013,799 + BBR_RS14440 0.56 -2.0 1,013,888 - BBR_RS14440 0.67 -2.9 1,013,897 - BBR_RS14440 0.68 +0.6 1,013,897 - BBR_RS14440 0.68 -0.8 1,013,930 - BBR_RS14440 0.72 +1.4 1,013,957 - BBR_RS14440 0.75 -0.4 1,013,968 + BBR_RS14440 0.77 -0.2 1,013,980 + BBR_RS14440 0.78 +0.6 1,013,990 + BBR_RS14440 0.79 -0.2 1,014,076 + +0.0 1,014,134 + +0.6 1,014,194 + -0.6 1,014,223 + -0.3 1,014,246 + -0.5 1,014,252 - -1.0 1,014,355 + -0.5 1,014,363 - -1.7 1,014,412 + +1.3 1,014,425 - +1.0 1,014,446 - -0.0 1,014,456 - -1.9 1,014,467 + +0.1 1,014,476 - +0.3 1,014,485 - -1.1 1,014,578 + BBR_RS14445 0.18 -0.6 1,014,601 + BBR_RS14445 0.20 +0.3 1,014,605 - BBR_RS14445 0.20 -0.7 1,014,634 + BBR_RS14445 0.23 -0.2 1,014,769 - BBR_RS14445 0.37 +0.4 1,014,769 - BBR_RS14445 0.37 +2.3 1,014,791 + BBR_RS14445 0.39 +0.6 1,014,793 + BBR_RS14445 0.40 +0.0 1,014,793 + BBR_RS14445 0.40 -0.5 1,014,801 + BBR_RS14445 0.40 -0.3 1,014,809 - BBR_RS14445 0.41 -0.8 1,014,854 + BBR_RS14445 0.46 +0.5 1,014,855 + BBR_RS14445 0.46 +0.2 1,014,855 - BBR_RS14445 0.46 +0.8 1,014,863 + BBR_RS14445 0.47 +0.0 1,014,887 + BBR_RS14445 0.49 +3.0 1,014,938 + BBR_RS14445 0.54 -0.5 1,014,938 + BBR_RS14445 0.54 +1.8 1,014,946 - BBR_RS14445 0.55 -0.3 1,014,946 - BBR_RS14445 0.55 -0.6 1,014,946 - BBR_RS14445 0.55 -0.9 1,014,946 - BBR_RS14445 0.55 +0.8 1,014,963 + BBR_RS14445 0.57 +0.4 1,014,976 + BBR_RS14445 0.58 +0.8 1,015,011 + BBR_RS14445 0.62 -0.0 1,015,019 - BBR_RS14445 0.63 +0.3 1,015,022 + BBR_RS14445 0.63 +0.6 1,015,022 + BBR_RS14445 0.63 -0.7 1,015,027 + BBR_RS14445 0.63 +0.4 1,015,029 - BBR_RS14445 0.64 -2.9 1,015,029 - BBR_RS14445 0.64 -1.8 1,015,029 - BBR_RS14445 0.64 +3.5 1,015,042 + BBR_RS14445 0.65 +0.3 1,015,071 + BBR_RS14445 0.68 -0.8 1,015,097 - BBR_RS14445 0.71 +0.1 1,015,157 + BBR_RS14445 0.77 +1.1 1,015,212 + BBR_RS14445 0.82 -1.5 1,015,276 - BBR_RS14445 0.89 +0.4 1,015,327 - -1.0 1,015,349 + +0.0 1,015,453 - +0.1 1,015,488 - -0.7 1,015,500 + -1.5 1,015,565 + +0.0 1,015,616 + +2.0 1,015,718 - BBR_RS14450 0.14 +0.1 1,015,737 - BBR_RS14450 0.15 +0.3 1,015,785 + BBR_RS14450 0.19 -0.2 1,015,785 + BBR_RS14450 0.19 -1.4 1,015,812 + BBR_RS14450 0.21 -1.0 1,015,892 + BBR_RS14450 0.28 +1.6 1,015,905 - BBR_RS14450 0.29 -0.1 1,015,933 + BBR_RS14450 0.32 -0.3 1,015,935 + BBR_RS14450 0.32 -0.0 1,015,977 + BBR_RS14450 0.35 -0.6 1,015,994 + BBR_RS14450 0.37 -0.4 1,015,995 - BBR_RS14450 0.37 -0.4 1,015,995 - BBR_RS14450 0.37 -0.5 1,015,995 - BBR_RS14450 0.37 -0.2 1,015,995 - BBR_RS14450 0.37 -0.5 1,015,995 - BBR_RS14450 0.37 -0.0 1,015,995 - BBR_RS14450 0.37 +0.4 1,016,000 - BBR_RS14450 0.37 +0.4 1,016,002 - BBR_RS14450 0.37 -0.0 1,016,002 - BBR_RS14450 0.37 +0.1 1,016,004 + BBR_RS14450 0.37 +0.7 1,016,013 - BBR_RS14450 0.38 +1.3 1,016,091 + BBR_RS14450 0.45 +0.4 1,016,109 + BBR_RS14450 0.46 +0.6 1,016,114 + BBR_RS14450 0.47 +0.2 1,016,120 + BBR_RS14450 0.47 +0.2 1,016,174 + BBR_RS14450 0.52 +1.2 1,016,239 + BBR_RS14450 0.57 -0.3 1,016,246 + BBR_RS14450 0.58 +0.2 1,016,246 + BBR_RS14450 0.58 -0.2 1,016,254 - BBR_RS14450 0.58 +1.0 1,016,304 + BBR_RS14450 0.62 +1.0 1,016,304 + BBR_RS14450 0.62 -0.3 1,016,322 + BBR_RS14450 0.64 +0.5 1,016,348 - BBR_RS14450 0.66 -1.0 1,016,371 + BBR_RS14450 0.68 -0.1 1,016,374 - BBR_RS14450 0.68 +1.0
Or see this region's nucleotide sequence