Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14000 and BBR_RS14005 are separated by 8 nucleotides BBR_RS14005 and BBR_RS14010 are separated by 155 nucleotides BBR_RS14010 and BBR_RS14015 overlap by 19 nucleotides
BBR_RS14000: BBR_RS14000 - hypothetical protein, at 917,337 to 919,169
_RS14000
BBR_RS14005: BBR_RS14005 - haloacid dehalogenase, at 919,178 to 920,224
_RS14005
BBR_RS14010: BBR_RS14010 - TlyA family rRNA (cytidine-2'-O)-methyltransferase, at 920,380 to 921,153
_RS14010
BBR_RS14015: BBR_RS14015 - hypothetical protein, at 921,135 to 921,809
_RS14015
Position (kb) 919
920
921 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 918.826 kb on + strand, within BBR_RS14000 at 918.868 kb on + strand, within BBR_RS14000 at 918.913 kb on + strand, within BBR_RS14000 at 918.913 kb on + strand, within BBR_RS14000 at 918.913 kb on + strand, within BBR_RS14000 at 918.921 kb on - strand, within BBR_RS14000 at 918.921 kb on - strand, within BBR_RS14000 at 918.922 kb on - strand, within BBR_RS14000 at 918.932 kb on + strand, within BBR_RS14000 at 918.947 kb on + strand, within BBR_RS14000 at 918.955 kb on - strand, within BBR_RS14000 at 918.967 kb on - strand, within BBR_RS14000 at 918.979 kb on - strand, within BBR_RS14000 at 918.985 kb on + strand, within BBR_RS14000 at 918.985 kb on + strand, within BBR_RS14000 at 918.985 kb on + strand, within BBR_RS14000 at 918.993 kb on - strand at 919.015 kb on + strand at 919.072 kb on + strand at 919.072 kb on + strand at 919.142 kb on + strand at 919.171 kb on - strand at 919.196 kb on + strand at 919.253 kb on + strand at 919.253 kb on + strand at 919.255 kb on + strand at 919.261 kb on - strand at 919.292 kb on + strand, within BBR_RS14005 at 919.355 kb on + strand, within BBR_RS14005 at 919.355 kb on + strand, within BBR_RS14005 at 919.381 kb on + strand, within BBR_RS14005 at 919.436 kb on - strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.548 kb on + strand, within BBR_RS14005 at 919.619 kb on - strand, within BBR_RS14005 at 919.621 kb on + strand, within BBR_RS14005 at 919.667 kb on + strand, within BBR_RS14005 at 919.697 kb on + strand, within BBR_RS14005 at 919.821 kb on + strand, within BBR_RS14005 at 919.946 kb on - strand, within BBR_RS14005 at 919.989 kb on + strand, within BBR_RS14005 at 920.018 kb on - strand, within BBR_RS14005 at 920.046 kb on + strand, within BBR_RS14005 at 920.046 kb on + strand, within BBR_RS14005 at 920.054 kb on - strand, within BBR_RS14005 at 920.099 kb on - strand, within BBR_RS14005 at 920.137 kb on + strand at 920.188 kb on - strand at 920.188 kb on - strand at 920.192 kb on + strand at 920.200 kb on - strand at 920.240 kb on - strand at 920.246 kb on + strand at 920.332 kb on + strand at 920.332 kb on + strand at 920.340 kb on - strand at 920.410 kb on - strand at 920.465 kb on - strand, within BBR_RS14010 at 920.471 kb on - strand, within BBR_RS14010 at 920.512 kb on - strand, within BBR_RS14010 at 920.551 kb on - strand, within BBR_RS14010 at 920.560 kb on - strand, within BBR_RS14010 at 920.619 kb on + strand, within BBR_RS14010 at 920.753 kb on + strand, within BBR_RS14010 at 920.777 kb on + strand, within BBR_RS14010 at 920.787 kb on - strand, within BBR_RS14010 at 920.812 kb on + strand, within BBR_RS14010 at 920.897 kb on + strand, within BBR_RS14010 at 920.952 kb on + strand, within BBR_RS14010 at 921.051 kb on - strand, within BBR_RS14010 at 921.051 kb on - strand, within BBR_RS14010 at 921.053 kb on - strand, within BBR_RS14010 at 921.068 kb on + strand, within BBR_RS14010 at 921.070 kb on - strand, within BBR_RS14010 at 921.075 kb on - strand, within BBR_RS14010 at 921.076 kb on - strand at 921.198 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 918,826 + BBR_RS14000 0.81 -3.2 918,868 + BBR_RS14000 0.84 -0.1 918,913 + BBR_RS14000 0.86 -0.8 918,913 + BBR_RS14000 0.86 -0.6 918,913 + BBR_RS14000 0.86 -1.0 918,921 - BBR_RS14000 0.86 -0.3 918,921 - BBR_RS14000 0.86 -0.1 918,922 - BBR_RS14000 0.86 -1.1 918,932 + BBR_RS14000 0.87 -0.7 918,947 + BBR_RS14000 0.88 +0.7 918,955 - BBR_RS14000 0.88 -0.5 918,967 - BBR_RS14000 0.89 -0.7 918,979 - BBR_RS14000 0.90 +1.8 918,985 + BBR_RS14000 0.90 +0.0 918,985 + BBR_RS14000 0.90 +0.5 918,985 + BBR_RS14000 0.90 -0.7 918,993 - +0.6 919,015 + +1.2 919,072 + -2.4 919,072 + +0.5 919,142 + +0.4 919,171 - -0.2 919,196 + +0.2 919,253 + +0.2 919,253 + +1.1 919,255 + -0.1 919,261 - +0.8 919,292 + BBR_RS14005 0.11 -0.1 919,355 + BBR_RS14005 0.17 +0.7 919,355 + BBR_RS14005 0.17 -0.9 919,381 + BBR_RS14005 0.19 +0.4 919,436 - BBR_RS14005 0.25 +0.9 919,486 + BBR_RS14005 0.29 +0.7 919,486 + BBR_RS14005 0.29 +0.3 919,486 + BBR_RS14005 0.29 -0.7 919,486 + BBR_RS14005 0.29 +1.0 919,486 + BBR_RS14005 0.29 -0.2 919,548 + BBR_RS14005 0.35 +0.6 919,619 - BBR_RS14005 0.42 -0.2 919,621 + BBR_RS14005 0.42 +0.1 919,667 + BBR_RS14005 0.47 -0.8 919,697 + BBR_RS14005 0.50 -0.1 919,821 + BBR_RS14005 0.61 +1.1 919,946 - BBR_RS14005 0.73 +1.5 919,989 + BBR_RS14005 0.77 +0.8 920,018 - BBR_RS14005 0.80 +1.0 920,046 + BBR_RS14005 0.83 -0.2 920,046 + BBR_RS14005 0.83 +0.8 920,054 - BBR_RS14005 0.84 -0.0 920,099 - BBR_RS14005 0.88 -0.2 920,137 + +1.3 920,188 - +0.4 920,188 - +0.3 920,192 + +0.0 920,200 - -0.2 920,240 - +0.3 920,246 + -3.4 920,332 + -0.6 920,332 + +0.0 920,340 - -0.0 920,410 - -1.0 920,465 - BBR_RS14010 0.11 -0.1 920,471 - BBR_RS14010 0.12 +0.1 920,512 - BBR_RS14010 0.17 +0.3 920,551 - BBR_RS14010 0.22 -0.2 920,560 - BBR_RS14010 0.23 -0.1 920,619 + BBR_RS14010 0.31 -0.2 920,753 + BBR_RS14010 0.48 -0.0 920,777 + BBR_RS14010 0.51 +0.3 920,787 - BBR_RS14010 0.53 +0.1 920,812 + BBR_RS14010 0.56 -0.7 920,897 + BBR_RS14010 0.67 +1.0 920,952 + BBR_RS14010 0.74 +0.1 921,051 - BBR_RS14010 0.87 +0.3 921,051 - BBR_RS14010 0.87 -0.1 921,053 - BBR_RS14010 0.87 -0.3 921,068 + BBR_RS14010 0.89 -0.3 921,070 - BBR_RS14010 0.89 +0.5 921,075 - BBR_RS14010 0.90 -1.7 921,076 - -0.2 921,198 + +0.5
Or see this region's nucleotide sequence