Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12905 and BBR_RS12910 are separated by 17 nucleotides BBR_RS12910 and BBR_RS20735 are separated by 411 nucleotides BBR_RS20735 and BBR_RS12915 are separated by 72 nucleotides
BBR_RS12905: BBR_RS12905 - galactose-1-phosphate uridylyltransferase, at 647,198 to 648,448
_RS12905
BBR_RS12910: BBR_RS12910 - galactokinase, at 648,466 to 649,716
_RS12910
BBR_RS20735: BBR_RS20735 - hypothetical protein, at 650,128 to 650,412
_RS20735
BBR_RS12915: BBR_RS12915 - glycine synthesis protein GlyXS <small>(from data)</small>, at 650,485 to 650,757
_RS12915
Position (kb) 648
649
650 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 647.765 kb on - strand, within BBR_RS12905 at 647.955 kb on - strand, within BBR_RS12905 at 648.176 kb on - strand, within BBR_RS12905 at 648.229 kb on - strand, within BBR_RS12905 at 648.483 kb on - strand at 648.483 kb on - strand at 648.489 kb on + strand at 648.497 kb on + strand at 648.505 kb on - strand at 648.505 kb on - strand at 648.505 kb on - strand at 648.505 kb on - strand at 648.505 kb on - strand at 648.540 kb on + strand at 648.553 kb on + strand at 648.555 kb on + strand at 648.561 kb on - strand at 648.563 kb on - strand at 648.572 kb on - strand at 648.572 kb on - strand at 648.572 kb on - strand at 648.572 kb on - strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.574 kb on + strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.582 kb on - strand at 648.584 kb on + strand at 648.584 kb on + strand at 648.592 kb on - strand, within BBR_RS12910 at 648.593 kb on + strand, within BBR_RS12910 at 648.618 kb on - strand, within BBR_RS12910 at 648.618 kb on - strand, within BBR_RS12910 at 648.649 kb on - strand, within BBR_RS12910 at 648.665 kb on + strand, within BBR_RS12910 at 648.665 kb on + strand, within BBR_RS12910 at 648.665 kb on + strand, within BBR_RS12910 at 648.673 kb on - strand, within BBR_RS12910 at 648.698 kb on - strand, within BBR_RS12910 at 648.712 kb on + strand, within BBR_RS12910 at 648.746 kb on + strand, within BBR_RS12910 at 648.746 kb on + strand, within BBR_RS12910 at 648.755 kb on - strand, within BBR_RS12910 at 648.783 kb on + strand, within BBR_RS12910 at 648.795 kb on + strand, within BBR_RS12910 at 648.816 kb on - strand, within BBR_RS12910 at 648.828 kb on + strand, within BBR_RS12910 at 648.828 kb on + strand, within BBR_RS12910 at 648.828 kb on + strand, within BBR_RS12910 at 648.828 kb on + strand, within BBR_RS12910 at 648.828 kb on + strand, within BBR_RS12910 at 648.828 kb on + strand, within BBR_RS12910 at 648.829 kb on + strand, within BBR_RS12910 at 648.836 kb on - strand, within BBR_RS12910 at 648.836 kb on - strand, within BBR_RS12910 at 648.837 kb on - strand, within BBR_RS12910 at 648.837 kb on - strand, within BBR_RS12910 at 648.850 kb on + strand, within BBR_RS12910 at 648.850 kb on + strand, within BBR_RS12910 at 648.856 kb on - strand, within BBR_RS12910 at 648.865 kb on + strand, within BBR_RS12910 at 648.872 kb on + strand, within BBR_RS12910 at 648.872 kb on + strand, within BBR_RS12910 at 648.872 kb on + strand, within BBR_RS12910 at 648.877 kb on + strand, within BBR_RS12910 at 648.880 kb on - strand, within BBR_RS12910 at 648.880 kb on - strand, within BBR_RS12910 at 648.895 kb on + strand, within BBR_RS12910 at 648.895 kb on + strand, within BBR_RS12910 at 648.895 kb on + strand, within BBR_RS12910 at 648.903 kb on - strand, within BBR_RS12910 at 648.903 kb on - strand, within BBR_RS12910 at 648.914 kb on + strand, within BBR_RS12910 at 648.931 kb on + strand, within BBR_RS12910 at 648.939 kb on - strand, within BBR_RS12910 at 648.939 kb on - strand, within BBR_RS12910 at 648.946 kb on - strand, within BBR_RS12910 at 648.946 kb on - strand, within BBR_RS12910 at 648.951 kb on - strand, within BBR_RS12910 at 648.968 kb on + strand, within BBR_RS12910 at 648.973 kb on + strand, within BBR_RS12910 at 648.976 kb on - strand, within BBR_RS12910 at 648.981 kb on - strand, within BBR_RS12910 at 648.988 kb on + strand, within BBR_RS12910 at 648.988 kb on + strand, within BBR_RS12910 at 648.990 kb on + strand, within BBR_RS12910 at 649.019 kb on - strand, within BBR_RS12910 at 649.019 kb on - strand, within BBR_RS12910 at 649.022 kb on + strand, within BBR_RS12910 at 649.022 kb on + strand, within BBR_RS12910 at 649.031 kb on + strand, within BBR_RS12910 at 649.031 kb on + strand, within BBR_RS12910 at 649.031 kb on + strand, within BBR_RS12910 at 649.039 kb on - strand, within BBR_RS12910 at 649.039 kb on - strand, within BBR_RS12910 at 649.053 kb on + strand, within BBR_RS12910 at 649.075 kb on - strand, within BBR_RS12910 at 649.121 kb on - strand, within BBR_RS12910 at 649.144 kb on - strand, within BBR_RS12910 at 649.144 kb on - strand, within BBR_RS12910 at 649.146 kb on + strand, within BBR_RS12910 at 649.146 kb on + strand, within BBR_RS12910 at 649.154 kb on - strand, within BBR_RS12910 at 649.154 kb on - strand, within BBR_RS12910 at 649.169 kb on - strand, within BBR_RS12910 at 649.203 kb on + strand, within BBR_RS12910 at 649.203 kb on + strand, within BBR_RS12910 at 649.223 kb on - strand, within BBR_RS12910 at 649.255 kb on + strand, within BBR_RS12910 at 649.255 kb on + strand, within BBR_RS12910 at 649.287 kb on + strand, within BBR_RS12910 at 649.295 kb on - strand, within BBR_RS12910 at 649.305 kb on + strand, within BBR_RS12910 at 649.305 kb on + strand, within BBR_RS12910 at 649.313 kb on - strand, within BBR_RS12910 at 649.313 kb on - strand, within BBR_RS12910 at 649.313 kb on - strand, within BBR_RS12910 at 649.408 kb on - strand, within BBR_RS12910 at 649.420 kb on - strand, within BBR_RS12910 at 649.420 kb on - strand, within BBR_RS12910 at 649.428 kb on + strand, within BBR_RS12910 at 649.428 kb on + strand, within BBR_RS12910 at 649.449 kb on + strand, within BBR_RS12910 at 649.496 kb on + strand, within BBR_RS12910 at 649.506 kb on + strand, within BBR_RS12910 at 649.522 kb on - strand, within BBR_RS12910 at 649.522 kb on - strand, within BBR_RS12910 at 649.533 kb on + strand, within BBR_RS12910 at 649.539 kb on + strand, within BBR_RS12910 at 649.559 kb on - strand, within BBR_RS12910 at 649.575 kb on - strand, within BBR_RS12910 at 649.594 kb on - strand at 649.597 kb on + strand at 649.602 kb on + strand at 649.605 kb on - strand at 649.607 kb on + strand at 649.631 kb on + strand at 649.640 kb on + strand at 649.666 kb on + strand at 649.685 kb on + strand at 649.718 kb on - strand at 649.747 kb on - strand at 649.753 kb on + strand at 649.757 kb on - strand at 649.778 kb on + strand at 649.778 kb on + strand at 649.786 kb on - strand at 649.786 kb on - strand at 649.788 kb on - strand at 649.860 kb on + strand at 649.862 kb on + strand at 649.868 kb on - strand at 649.872 kb on + strand at 649.882 kb on + strand at 649.909 kb on + strand at 649.917 kb on - strand at 650.020 kb on - strand at 650.034 kb on + strand at 650.072 kb on + strand at 650.072 kb on + strand at 650.073 kb on - strand at 650.085 kb on - strand at 650.172 kb on + strand, within BBR_RS20735 at 650.199 kb on - strand, within BBR_RS20735 at 650.199 kb on - strand, within BBR_RS20735 at 650.224 kb on + strand, within BBR_RS20735 at 650.227 kb on + strand, within BBR_RS20735 at 650.232 kb on + strand, within BBR_RS20735 at 650.258 kb on + strand, within BBR_RS20735 at 650.259 kb on - strand, within BBR_RS20735 at 650.275 kb on + strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.357 kb on - strand, within BBR_RS20735 at 650.357 kb on - strand, within BBR_RS20735 at 650.363 kb on + strand, within BBR_RS20735 at 650.374 kb on + strand, within BBR_RS20735 at 650.388 kb on + strand at 650.389 kb on - strand at 650.433 kb on + strand at 650.435 kb on + strand at 650.443 kb on - strand at 650.443 kb on - strand at 650.451 kb on - strand at 650.451 kb on - strand at 650.476 kb on + strand at 650.478 kb on - strand at 650.480 kb on - strand at 650.493 kb on + strand at 650.494 kb on + strand at 650.512 kb on + strand at 650.541 kb on + strand, within BBR_RS12915 at 650.541 kb on + strand, within BBR_RS12915 at 650.546 kb on + strand, within BBR_RS12915 at 650.546 kb on + strand, within BBR_RS12915 at 650.549 kb on - strand, within BBR_RS12915 at 650.567 kb on + strand, within BBR_RS12915 at 650.567 kb on + strand, within BBR_RS12915 at 650.660 kb on + strand, within BBR_RS12915
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 647,765 - BBR_RS12905 0.45 +3.1 647,955 - BBR_RS12905 0.61 +0.8 648,176 - BBR_RS12905 0.78 +1.7 648,229 - BBR_RS12905 0.82 -1.8 648,483 - +0.1 648,483 - +0.5 648,489 + +0.5 648,497 + -0.4 648,505 - +0.9 648,505 - +0.1 648,505 - +0.0 648,505 - +0.2 648,505 - -0.1 648,540 + +0.1 648,553 + +0.2 648,555 + +0.4 648,561 - -0.2 648,563 - +0.5 648,572 - +0.6 648,572 - +0.8 648,572 - -0.1 648,572 - +0.4 648,574 + +1.2 648,574 + +0.4 648,574 + +0.0 648,574 + -0.1 648,574 + +0.3 648,574 + +0.1 648,574 + +1.1 648,574 + -0.1 648,574 + +0.1 648,574 + +0.6 648,574 + +0.0 648,574 + -0.1 648,574 + +0.4 648,574 + +0.2 648,574 + +0.2 648,574 + +0.5 648,574 + +0.0 648,582 - -0.7 648,582 - -0.8 648,582 - +0.4 648,582 - +0.2 648,582 - -0.6 648,582 - +0.6 648,582 - +1.5 648,582 - +0.3 648,582 - -0.3 648,584 + +0.2 648,584 + +0.4 648,592 - BBR_RS12910 0.10 +0.2 648,593 + BBR_RS12910 0.10 +0.5 648,618 - BBR_RS12910 0.12 +0.1 648,618 - BBR_RS12910 0.12 +0.4 648,649 - BBR_RS12910 0.15 +0.6 648,665 + BBR_RS12910 0.16 +0.1 648,665 + BBR_RS12910 0.16 +0.3 648,665 + BBR_RS12910 0.16 +0.1 648,673 - BBR_RS12910 0.17 +0.3 648,698 - BBR_RS12910 0.19 +0.7 648,712 + BBR_RS12910 0.20 +0.6 648,746 + BBR_RS12910 0.22 -0.0 648,746 + BBR_RS12910 0.22 -0.0 648,755 - BBR_RS12910 0.23 +0.5 648,783 + BBR_RS12910 0.25 +0.7 648,795 + BBR_RS12910 0.26 +0.2 648,816 - BBR_RS12910 0.28 +0.9 648,828 + BBR_RS12910 0.29 +0.6 648,828 + BBR_RS12910 0.29 +0.0 648,828 + BBR_RS12910 0.29 -0.1 648,828 + BBR_RS12910 0.29 -0.1 648,828 + BBR_RS12910 0.29 +0.2 648,828 + BBR_RS12910 0.29 +0.9 648,829 + BBR_RS12910 0.29 +1.5 648,836 - BBR_RS12910 0.30 +0.5 648,836 - BBR_RS12910 0.30 +0.7 648,837 - BBR_RS12910 0.30 +0.4 648,837 - BBR_RS12910 0.30 +0.5 648,850 + BBR_RS12910 0.31 +0.0 648,850 + BBR_RS12910 0.31 +0.4 648,856 - BBR_RS12910 0.31 +0.6 648,865 + BBR_RS12910 0.32 +0.2 648,872 + BBR_RS12910 0.32 +0.4 648,872 + BBR_RS12910 0.32 +0.3 648,872 + BBR_RS12910 0.32 -0.1 648,877 + BBR_RS12910 0.33 +0.1 648,880 - BBR_RS12910 0.33 +1.1 648,880 - BBR_RS12910 0.33 -0.2 648,895 + BBR_RS12910 0.34 +0.5 648,895 + BBR_RS12910 0.34 +0.1 648,895 + BBR_RS12910 0.34 -0.2 648,903 - BBR_RS12910 0.35 +0.8 648,903 - BBR_RS12910 0.35 +1.1 648,914 + BBR_RS12910 0.36 +0.1 648,931 + BBR_RS12910 0.37 -0.1 648,939 - BBR_RS12910 0.38 -0.1 648,939 - BBR_RS12910 0.38 +0.0 648,946 - BBR_RS12910 0.38 +0.7 648,946 - BBR_RS12910 0.38 +0.5 648,951 - BBR_RS12910 0.39 -0.4 648,968 + BBR_RS12910 0.40 +0.2 648,973 + BBR_RS12910 0.41 +0.6 648,976 - BBR_RS12910 0.41 -0.2 648,981 - BBR_RS12910 0.41 +0.1 648,988 + BBR_RS12910 0.42 +0.1 648,988 + BBR_RS12910 0.42 -0.1 648,990 + BBR_RS12910 0.42 -0.1 649,019 - BBR_RS12910 0.44 -0.5 649,019 - BBR_RS12910 0.44 -0.1 649,022 + BBR_RS12910 0.44 +0.5 649,022 + BBR_RS12910 0.44 +0.4 649,031 + BBR_RS12910 0.45 +0.8 649,031 + BBR_RS12910 0.45 -1.8 649,031 + BBR_RS12910 0.45 +0.9 649,039 - BBR_RS12910 0.46 +0.3 649,039 - BBR_RS12910 0.46 +0.1 649,053 + BBR_RS12910 0.47 +0.1 649,075 - BBR_RS12910 0.49 -0.3 649,121 - BBR_RS12910 0.52 +0.4 649,144 - BBR_RS12910 0.54 +0.4 649,144 - BBR_RS12910 0.54 +0.1 649,146 + BBR_RS12910 0.54 +0.9 649,146 + BBR_RS12910 0.54 +0.1 649,154 - BBR_RS12910 0.55 +0.1 649,154 - BBR_RS12910 0.55 +0.8 649,169 - BBR_RS12910 0.56 -0.1 649,203 + BBR_RS12910 0.59 +0.4 649,203 + BBR_RS12910 0.59 +0.8 649,223 - BBR_RS12910 0.61 +0.3 649,255 + BBR_RS12910 0.63 +0.3 649,255 + BBR_RS12910 0.63 -0.5 649,287 + BBR_RS12910 0.66 +1.0 649,295 - BBR_RS12910 0.66 +0.6 649,305 + BBR_RS12910 0.67 +0.1 649,305 + BBR_RS12910 0.67 +1.5 649,313 - BBR_RS12910 0.68 -1.4 649,313 - BBR_RS12910 0.68 +0.1 649,313 - BBR_RS12910 0.68 +0.4 649,408 - BBR_RS12910 0.75 -0.2 649,420 - BBR_RS12910 0.76 +0.0 649,420 - BBR_RS12910 0.76 +0.3 649,428 + BBR_RS12910 0.77 -0.0 649,428 + BBR_RS12910 0.77 -0.0 649,449 + BBR_RS12910 0.79 +0.4 649,496 + BBR_RS12910 0.82 +0.2 649,506 + BBR_RS12910 0.83 +0.8 649,522 - BBR_RS12910 0.84 -0.1 649,522 - BBR_RS12910 0.84 +0.6 649,533 + BBR_RS12910 0.85 +0.8 649,539 + BBR_RS12910 0.86 -0.0 649,559 - BBR_RS12910 0.87 +1.2 649,575 - BBR_RS12910 0.89 -0.3 649,594 - -0.4 649,597 + -0.0 649,602 + -0.1 649,605 - +1.0 649,607 + +0.9 649,631 + -0.0 649,640 + +0.9 649,666 + +0.1 649,685 + +0.2 649,718 - +0.4 649,747 - -0.9 649,753 + -0.3 649,757 - +0.9 649,778 + -0.1 649,778 + -0.5 649,786 - -0.0 649,786 - -0.8 649,788 - +0.5 649,860 + +0.9 649,862 + +0.4 649,868 - +0.2 649,872 + -0.1 649,882 + +0.3 649,909 + +1.5 649,917 - +0.2 650,020 - +0.4 650,034 + -0.5 650,072 + +0.1 650,072 + -0.0 650,073 - +2.0 650,085 - -0.5 650,172 + BBR_RS20735 0.15 +0.3 650,199 - BBR_RS20735 0.25 +0.6 650,199 - BBR_RS20735 0.25 +0.2 650,224 + BBR_RS20735 0.34 +0.5 650,227 + BBR_RS20735 0.35 +0.6 650,232 + BBR_RS20735 0.36 -0.3 650,258 + BBR_RS20735 0.46 -0.5 650,259 - BBR_RS20735 0.46 -0.6 650,275 + BBR_RS20735 0.52 +0.9 650,347 - BBR_RS20735 0.77 +0.3 650,347 - BBR_RS20735 0.77 +1.4 650,347 - BBR_RS20735 0.77 -1.0 650,347 - BBR_RS20735 0.77 -0.1 650,349 + BBR_RS20735 0.78 -0.3 650,349 + BBR_RS20735 0.78 -2.8 650,349 + BBR_RS20735 0.78 -0.9 650,349 + BBR_RS20735 0.78 -1.2 650,349 + BBR_RS20735 0.78 -1.8 650,357 - BBR_RS20735 0.80 +1.5 650,357 - BBR_RS20735 0.80 +0.9 650,363 + BBR_RS20735 0.82 -0.7 650,374 + BBR_RS20735 0.86 -0.1 650,388 + +0.3 650,389 - +0.7 650,433 + +0.2 650,435 + -0.4 650,443 - -0.4 650,443 - -1.2 650,451 - +1.5 650,451 - -0.2 650,476 + +1.2 650,478 - -0.4 650,480 - -0.1 650,493 + -1.9 650,494 + +0.3 650,512 + -0.7 650,541 + BBR_RS12915 0.21 -0.2 650,541 + BBR_RS12915 0.21 -0.1 650,546 + BBR_RS12915 0.22 -0.4 650,546 + BBR_RS12915 0.22 -0.2 650,549 - BBR_RS12915 0.23 +0.1 650,567 + BBR_RS12915 0.30 -1.3 650,567 + BBR_RS12915 0.30 +0.8 650,660 + BBR_RS12915 0.64 -0.6
Or see this region's nucleotide sequence