Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12705 and BBR_RS12710 are separated by 8 nucleotides BBR_RS12710 and BBR_RS12715 are separated by 482 nucleotides BBR_RS12715 and BBR_RS12720 are separated by 181 nucleotides BBR_RS12720 and BBR_RS12725 are separated by 140 nucleotides BBR_RS12725 and BBR_RS20515 are separated by 64 nucleotides
BBR_RS12705: BBR_RS12705 - hypothetical protein, at 605,361 to 606,662
_RS12705
BBR_RS12710: BBR_RS12710 - hypothetical protein, at 606,671 to 606,916
_RS12710
BBR_RS12715: BBR_RS12715 - helix-turn-helix domain-containing protein, at 607,399 to 607,782
_RS12715
BBR_RS12720: BBR_RS12720 - hypothetical protein, at 607,964 to 608,050
_RS12720
BBR_RS12725: BBR_RS12725 - hypothetical protein, at 608,191 to 608,571
_RS12725
BBR_RS20515: BBR_RS20515 - transposase, at 608,636 to 609,697
_RS20515
Position (kb) 607
608 Strain fitness (log2 ratio) -1
0
1
2 at 606.408 kb on + strand, within BBR_RS12705 at 606.413 kb on - strand, within BBR_RS12705 at 606.422 kb on + strand, within BBR_RS12705 at 606.439 kb on + strand, within BBR_RS12705 at 606.439 kb on + strand, within BBR_RS12705 at 606.439 kb on + strand, within BBR_RS12705 at 606.439 kb on + strand, within BBR_RS12705 at 606.439 kb on + strand, within BBR_RS12705 at 606.439 kb on + strand, within BBR_RS12705 at 606.439 kb on + strand, within BBR_RS12705 at 606.447 kb on - strand, within BBR_RS12705 at 606.447 kb on - strand, within BBR_RS12705 at 606.447 kb on - strand, within BBR_RS12705 at 606.447 kb on - strand, within BBR_RS12705 at 606.486 kb on + strand, within BBR_RS12705 at 606.486 kb on + strand, within BBR_RS12705 at 606.486 kb on + strand, within BBR_RS12705 at 606.486 kb on + strand, within BBR_RS12705 at 606.486 kb on + strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.494 kb on - strand, within BBR_RS12705 at 606.505 kb on + strand, within BBR_RS12705 at 606.597 kb on + strand at 606.597 kb on + strand at 606.602 kb on + strand at 606.668 kb on + strand at 606.668 kb on + strand at 606.784 kb on + strand, within BBR_RS12710 at 606.790 kb on + strand, within BBR_RS12710 at 606.790 kb on + strand, within BBR_RS12710 at 606.794 kb on - strand, within BBR_RS12710 at 606.799 kb on - strand, within BBR_RS12710 at 606.800 kb on + strand, within BBR_RS12710 at 606.808 kb on - strand, within BBR_RS12710 at 606.866 kb on + strand, within BBR_RS12710 at 606.874 kb on - strand, within BBR_RS12710 at 606.889 kb on + strand, within BBR_RS12710 at 606.902 kb on - strand at 606.902 kb on - strand at 606.923 kb on + strand at 606.963 kb on + strand at 606.971 kb on - strand at 606.972 kb on - strand at 607.017 kb on + strand at 607.025 kb on - strand at 607.084 kb on - strand at 607.085 kb on + strand at 607.108 kb on - strand at 607.117 kb on - strand at 607.123 kb on + strand at 607.123 kb on + strand at 607.123 kb on + strand at 607.123 kb on + strand at 607.131 kb on - strand at 607.145 kb on + strand at 607.147 kb on + strand at 607.179 kb on + strand at 607.211 kb on - strand at 607.216 kb on + strand at 607.224 kb on - strand at 607.229 kb on - strand at 607.256 kb on + strand at 607.256 kb on + strand at 607.305 kb on - strand at 607.310 kb on + strand at 607.401 kb on + strand at 607.401 kb on + strand at 607.409 kb on - strand at 607.432 kb on - strand at 607.437 kb on - strand at 607.437 kb on - strand at 607.442 kb on - strand, within BBR_RS12715 at 607.456 kb on - strand, within BBR_RS12715 at 607.468 kb on - strand, within BBR_RS12715 at 607.518 kb on + strand, within BBR_RS12715 at 607.540 kb on + strand, within BBR_RS12715 at 607.543 kb on + strand, within BBR_RS12715 at 607.577 kb on + strand, within BBR_RS12715 at 607.577 kb on + strand, within BBR_RS12715 at 607.597 kb on + strand, within BBR_RS12715 at 607.600 kb on - strand, within BBR_RS12715 at 607.600 kb on - strand, within BBR_RS12715 at 607.640 kb on + strand, within BBR_RS12715 at 607.653 kb on - strand, within BBR_RS12715 at 607.668 kb on + strand, within BBR_RS12715 at 607.676 kb on - strand, within BBR_RS12715 at 607.759 kb on - strand at 607.791 kb on + strand at 607.800 kb on - strand at 607.818 kb on + strand at 607.927 kb on - strand at 608.020 kb on - strand, within BBR_RS12720 at 608.021 kb on - strand, within BBR_RS12720 at 608.031 kb on - strand, within BBR_RS12720 at 608.117 kb on - strand at 608.117 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.152 kb on + strand at 608.181 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.193 kb on + strand at 608.201 kb on - strand at 608.201 kb on - strand at 608.211 kb on + strand at 608.231 kb on + strand, within BBR_RS12725 at 608.239 kb on - strand, within BBR_RS12725 at 608.347 kb on + strand, within BBR_RS12725 at 608.355 kb on - strand, within BBR_RS12725 at 608.357 kb on - strand, within BBR_RS12725 at 608.357 kb on - strand, within BBR_RS12725 at 608.379 kb on + strand, within BBR_RS12725 at 608.382 kb on - strand, within BBR_RS12725 at 608.397 kb on + strand, within BBR_RS12725 at 608.405 kb on - strand, within BBR_RS12725 at 608.424 kb on - strand, within BBR_RS12725 at 608.502 kb on - strand, within BBR_RS12725 at 608.553 kb on + strand at 608.555 kb on + strand at 608.555 kb on + strand at 608.555 kb on + strand at 608.561 kb on - strand at 608.563 kb on - strand at 608.563 kb on - strand at 608.599 kb on + strand at 608.602 kb on + strand at 608.604 kb on + strand at 608.662 kb on - strand at 608.663 kb on + strand at 608.663 kb on + strand at 608.671 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.749 kb on - strand, within BBR_RS20515 at 608.767 kb on + strand, within BBR_RS20515 at 608.767 kb on - strand, within BBR_RS20515
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 606,408 + BBR_RS12705 0.80 -0.6 606,413 - BBR_RS12705 0.81 -1.4 606,422 + BBR_RS12705 0.81 +0.9 606,439 + BBR_RS12705 0.83 +0.2 606,439 + BBR_RS12705 0.83 -0.4 606,439 + BBR_RS12705 0.83 -0.3 606,439 + BBR_RS12705 0.83 -0.4 606,439 + BBR_RS12705 0.83 +0.2 606,439 + BBR_RS12705 0.83 -0.2 606,439 + BBR_RS12705 0.83 -0.7 606,447 - BBR_RS12705 0.83 -1.1 606,447 - BBR_RS12705 0.83 +0.9 606,447 - BBR_RS12705 0.83 -0.0 606,447 - BBR_RS12705 0.83 -0.4 606,486 + BBR_RS12705 0.86 +0.7 606,486 + BBR_RS12705 0.86 -0.2 606,486 + BBR_RS12705 0.86 +0.5 606,486 + BBR_RS12705 0.86 -1.0 606,486 + BBR_RS12705 0.86 -0.2 606,494 - BBR_RS12705 0.87 -1.1 606,494 - BBR_RS12705 0.87 +2.4 606,494 - BBR_RS12705 0.87 +0.1 606,494 - BBR_RS12705 0.87 -0.6 606,494 - BBR_RS12705 0.87 +0.2 606,494 - BBR_RS12705 0.87 -1.5 606,494 - BBR_RS12705 0.87 +0.4 606,494 - BBR_RS12705 0.87 +0.4 606,494 - BBR_RS12705 0.87 +0.1 606,505 + BBR_RS12705 0.88 +0.3 606,597 + -0.1 606,597 + -0.8 606,602 + +0.5 606,668 + -1.2 606,668 + +1.1 606,784 + BBR_RS12710 0.46 +0.3 606,790 + BBR_RS12710 0.48 +0.3 606,790 + BBR_RS12710 0.48 -1.0 606,794 - BBR_RS12710 0.50 -0.8 606,799 - BBR_RS12710 0.52 +0.2 606,800 + BBR_RS12710 0.52 -0.1 606,808 - BBR_RS12710 0.56 +0.6 606,866 + BBR_RS12710 0.79 -0.2 606,874 - BBR_RS12710 0.83 +0.4 606,889 + BBR_RS12710 0.89 -0.6 606,902 - -0.4 606,902 - +0.4 606,923 + -0.5 606,963 + -1.2 606,971 - -0.7 606,972 - -0.1 607,017 + +0.0 607,025 - +0.1 607,084 - +0.6 607,085 + +0.2 607,108 - +1.2 607,117 - +0.6 607,123 + +0.8 607,123 + -0.1 607,123 + +0.2 607,123 + +1.2 607,131 - -0.8 607,145 + +2.0 607,147 + -1.5 607,179 + +0.3 607,211 - +1.8 607,216 + +1.0 607,224 - -0.6 607,229 - +0.7 607,256 + +0.1 607,256 + +0.0 607,305 - -1.5 607,310 + -1.1 607,401 + +0.2 607,401 + +0.7 607,409 - +0.5 607,432 - +0.3 607,437 - -0.9 607,437 - -0.5 607,442 - BBR_RS12715 0.11 +0.5 607,456 - BBR_RS12715 0.15 +0.1 607,468 - BBR_RS12715 0.18 -0.8 607,518 + BBR_RS12715 0.31 -0.5 607,540 + BBR_RS12715 0.37 +0.1 607,543 + BBR_RS12715 0.38 +0.2 607,577 + BBR_RS12715 0.46 +0.9 607,577 + BBR_RS12715 0.46 +0.4 607,597 + BBR_RS12715 0.52 +0.2 607,600 - BBR_RS12715 0.52 +1.6 607,600 - BBR_RS12715 0.52 +0.8 607,640 + BBR_RS12715 0.63 +0.7 607,653 - BBR_RS12715 0.66 -0.9 607,668 + BBR_RS12715 0.70 -0.3 607,676 - BBR_RS12715 0.72 -0.1 607,759 - -0.3 607,791 + +1.8 607,800 - +0.3 607,818 + -1.3 607,927 - +0.1 608,020 - BBR_RS12720 0.64 +0.5 608,021 - BBR_RS12720 0.66 +0.3 608,031 - BBR_RS12720 0.77 -1.1 608,117 - +0.7 608,117 - +0.6 608,123 - -0.4 608,123 - +1.8 608,123 - +0.2 608,123 - +0.6 608,152 + +0.8 608,181 - -0.8 608,191 - +0.6 608,191 - -0.1 608,191 - +0.1 608,191 - +0.2 608,193 + +0.7 608,201 - -0.3 608,201 - +0.2 608,211 + +0.3 608,231 + BBR_RS12725 0.10 -0.2 608,239 - BBR_RS12725 0.13 -0.3 608,347 + BBR_RS12725 0.41 -0.7 608,355 - BBR_RS12725 0.43 +0.1 608,357 - BBR_RS12725 0.44 -0.3 608,357 - BBR_RS12725 0.44 +0.0 608,379 + BBR_RS12725 0.49 +1.0 608,382 - BBR_RS12725 0.50 -0.3 608,397 + BBR_RS12725 0.54 +1.4 608,405 - BBR_RS12725 0.56 +0.1 608,424 - BBR_RS12725 0.61 +0.5 608,502 - BBR_RS12725 0.82 -0.0 608,553 + +1.3 608,555 + +0.1 608,555 + +0.7 608,555 + -0.1 608,561 - -0.3 608,563 - -0.2 608,563 - -0.4 608,599 + +1.2 608,602 + +0.3 608,604 + +0.1 608,662 - -0.4 608,663 + -1.0 608,663 + -0.1 608,671 - +1.2 608,676 - -0.5 608,676 - -0.6 608,676 - +0.2 608,676 - -0.2 608,676 - -0.9 608,676 - +0.9 608,749 - BBR_RS20515 0.11 -0.3 608,767 + BBR_RS20515 0.12 -0.5 608,767 - BBR_RS20515 0.12 +0.2
Or see this region's nucleotide sequence