Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12535 and BBR_RS12540 are separated by 40 nucleotides BBR_RS12540 and BBR_RS12545 are separated by 367 nucleotides
BBR_RS12535: BBR_RS12535 - divalent metal cation transporter MntH, at 567,972 to 569,297
_RS12535
BBR_RS12540: BBR_RS12540 - MFS transporter, at 569,338 to 570,936
_RS12540
BBR_RS12545: BBR_RS12545 - DUF4012 domain-containing protein, at 571,304 to 573,064
_RS12545
Position (kb) 569
570
571 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 568.339 kb on + strand, within BBR_RS12535 at 568.349 kb on + strand, within BBR_RS12535 at 568.356 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.423 kb on - strand, within BBR_RS12535 at 568.475 kb on - strand, within BBR_RS12535 at 568.481 kb on + strand, within BBR_RS12535 at 568.481 kb on + strand, within BBR_RS12535 at 568.502 kb on + strand, within BBR_RS12535 at 568.502 kb on + strand, within BBR_RS12535 at 568.504 kb on + strand, within BBR_RS12535 at 568.515 kb on - strand, within BBR_RS12535 at 568.515 kb on - strand, within BBR_RS12535 at 568.547 kb on - strand, within BBR_RS12535 at 568.568 kb on + strand, within BBR_RS12535 at 568.576 kb on - strand, within BBR_RS12535 at 568.576 kb on - strand, within BBR_RS12535 at 568.604 kb on - strand, within BBR_RS12535 at 568.619 kb on + strand, within BBR_RS12535 at 568.619 kb on + strand, within BBR_RS12535 at 568.634 kb on - strand, within BBR_RS12535 at 568.638 kb on + strand, within BBR_RS12535 at 568.648 kb on - strand, within BBR_RS12535 at 568.692 kb on - strand, within BBR_RS12535 at 568.696 kb on + strand, within BBR_RS12535 at 568.714 kb on + strand, within BBR_RS12535 at 568.716 kb on + strand, within BBR_RS12535 at 568.717 kb on + strand, within BBR_RS12535 at 568.736 kb on + strand, within BBR_RS12535 at 568.762 kb on - strand, within BBR_RS12535 at 568.762 kb on - strand, within BBR_RS12535 at 568.786 kb on - strand, within BBR_RS12535 at 568.827 kb on - strand, within BBR_RS12535 at 568.842 kb on + strand, within BBR_RS12535 at 568.898 kb on + strand, within BBR_RS12535 at 568.965 kb on - strand, within BBR_RS12535 at 569.004 kb on + strand, within BBR_RS12535 at 569.024 kb on + strand, within BBR_RS12535 at 569.032 kb on - strand, within BBR_RS12535 at 569.033 kb on - strand, within BBR_RS12535 at 569.035 kb on + strand, within BBR_RS12535 at 569.038 kb on - strand, within BBR_RS12535 at 569.038 kb on - strand, within BBR_RS12535 at 569.053 kb on + strand, within BBR_RS12535 at 569.077 kb on - strand, within BBR_RS12535 at 569.077 kb on - strand, within BBR_RS12535 at 569.113 kb on + strand, within BBR_RS12535 at 569.130 kb on + strand, within BBR_RS12535 at 569.130 kb on + strand, within BBR_RS12535 at 569.143 kb on + strand, within BBR_RS12535 at 569.151 kb on - strand, within BBR_RS12535 at 569.189 kb on + strand at 569.189 kb on - strand at 569.189 kb on - strand at 569.228 kb on - strand at 569.272 kb on - strand at 569.277 kb on + strand at 569.279 kb on - strand at 569.279 kb on - strand at 569.285 kb on - strand at 569.313 kb on + strand at 569.343 kb on + strand at 569.353 kb on - strand at 569.353 kb on - strand at 569.353 kb on - strand at 569.392 kb on - strand at 569.406 kb on - strand at 569.411 kb on - strand at 569.463 kb on + strand at 569.463 kb on + strand at 569.463 kb on + strand at 569.463 kb on + strand at 569.463 kb on - strand at 569.471 kb on - strand at 569.471 kb on - strand at 569.481 kb on - strand at 569.505 kb on - strand, within BBR_RS12540 at 569.541 kb on + strand, within BBR_RS12540 at 569.571 kb on - strand, within BBR_RS12540 at 569.606 kb on + strand, within BBR_RS12540 at 569.608 kb on + strand, within BBR_RS12540 at 569.645 kb on - strand, within BBR_RS12540 at 569.645 kb on - strand, within BBR_RS12540 at 569.775 kb on + strand, within BBR_RS12540 at 569.775 kb on - strand, within BBR_RS12540 at 569.868 kb on + strand, within BBR_RS12540 at 569.876 kb on - strand, within BBR_RS12540 at 569.897 kb on - strand, within BBR_RS12540 at 569.955 kb on + strand, within BBR_RS12540 at 569.955 kb on + strand, within BBR_RS12540 at 570.003 kb on + strand, within BBR_RS12540 at 570.013 kb on - strand, within BBR_RS12540 at 570.013 kb on - strand, within BBR_RS12540 at 570.035 kb on - strand, within BBR_RS12540 at 570.035 kb on - strand, within BBR_RS12540 at 570.042 kb on + strand, within BBR_RS12540 at 570.047 kb on + strand, within BBR_RS12540 at 570.055 kb on - strand, within BBR_RS12540 at 570.059 kb on + strand, within BBR_RS12540 at 570.108 kb on - strand, within BBR_RS12540 at 570.143 kb on + strand, within BBR_RS12540 at 570.143 kb on + strand, within BBR_RS12540 at 570.151 kb on - strand, within BBR_RS12540 at 570.151 kb on - strand, within BBR_RS12540 at 570.162 kb on - strand, within BBR_RS12540 at 570.162 kb on - strand, within BBR_RS12540 at 570.168 kb on + strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.217 kb on + strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540 at 570.308 kb on + strand, within BBR_RS12540 at 570.319 kb on - strand, within BBR_RS12540 at 570.341 kb on - strand, within BBR_RS12540 at 570.350 kb on + strand, within BBR_RS12540 at 570.423 kb on + strand, within BBR_RS12540 at 570.423 kb on + strand, within BBR_RS12540 at 570.437 kb on + strand, within BBR_RS12540 at 570.437 kb on + strand, within BBR_RS12540 at 570.445 kb on - strand, within BBR_RS12540 at 570.445 kb on - strand, within BBR_RS12540 at 570.445 kb on - strand, within BBR_RS12540 at 570.446 kb on + strand, within BBR_RS12540 at 570.487 kb on - strand, within BBR_RS12540 at 570.502 kb on + strand, within BBR_RS12540 at 570.507 kb on - strand, within BBR_RS12540 at 570.512 kb on + strand, within BBR_RS12540 at 570.512 kb on + strand, within BBR_RS12540 at 570.512 kb on + strand, within BBR_RS12540 at 570.515 kb on - strand, within BBR_RS12540 at 570.517 kb on + strand, within BBR_RS12540 at 570.525 kb on - strand, within BBR_RS12540 at 570.525 kb on - strand, within BBR_RS12540 at 570.548 kb on + strand, within BBR_RS12540 at 570.571 kb on + strand, within BBR_RS12540 at 570.576 kb on + strand, within BBR_RS12540 at 570.576 kb on + strand, within BBR_RS12540 at 570.686 kb on + strand, within BBR_RS12540 at 570.695 kb on + strand, within BBR_RS12540 at 570.699 kb on - strand, within BBR_RS12540 at 570.720 kb on + strand, within BBR_RS12540 at 570.720 kb on + strand, within BBR_RS12540 at 570.837 kb on + strand at 570.853 kb on - strand at 570.880 kb on + strand at 570.881 kb on - strand at 570.888 kb on - strand at 570.888 kb on - strand at 570.888 kb on - strand at 570.919 kb on + strand at 570.940 kb on + strand at 570.940 kb on - strand at 570.940 kb on - strand at 570.946 kb on - strand at 571.007 kb on - strand at 571.060 kb on + strand at 571.060 kb on - strand at 571.088 kb on + strand at 571.088 kb on + strand at 571.088 kb on + strand at 571.096 kb on - strand at 571.102 kb on + strand at 571.102 kb on + strand at 571.102 kb on + strand at 571.102 kb on + strand at 571.102 kb on + strand at 571.103 kb on + strand at 571.103 kb on - strand at 571.103 kb on - strand at 571.110 kb on - strand at 571.110 kb on - strand at 571.110 kb on - strand at 571.110 kb on - strand at 571.110 kb on - strand at 571.110 kb on - strand at 571.110 kb on - strand at 571.122 kb on + strand at 571.131 kb on + strand at 571.168 kb on - strand at 571.193 kb on - strand at 571.211 kb on - strand at 571.211 kb on - strand at 571.211 kb on - strand at 571.211 kb on - strand at 571.464 kb on + strand at 571.464 kb on - strand at 571.464 kb on - strand at 571.500 kb on + strand, within BBR_RS12545 at 571.534 kb on + strand, within BBR_RS12545 at 571.615 kb on + strand, within BBR_RS12545 at 571.783 kb on + strand, within BBR_RS12545 at 571.813 kb on + strand, within BBR_RS12545 at 571.923 kb on + strand, within BBR_RS12545
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 568,339 + BBR_RS12535 0.28 -1.6 568,349 + BBR_RS12535 0.28 -0.5 568,356 - BBR_RS12535 0.29 +0.1 568,357 - BBR_RS12535 0.29 +0.2 568,357 - BBR_RS12535 0.29 +1.0 568,357 - BBR_RS12535 0.29 +0.1 568,423 - BBR_RS12535 0.34 +0.5 568,475 - BBR_RS12535 0.38 -1.3 568,481 + BBR_RS12535 0.38 -0.3 568,481 + BBR_RS12535 0.38 +0.7 568,502 + BBR_RS12535 0.40 +0.4 568,502 + BBR_RS12535 0.40 -0.6 568,504 + BBR_RS12535 0.40 +1.2 568,515 - BBR_RS12535 0.41 +0.5 568,515 - BBR_RS12535 0.41 +0.4 568,547 - BBR_RS12535 0.43 +0.5 568,568 + BBR_RS12535 0.45 -0.4 568,576 - BBR_RS12535 0.46 -0.1 568,576 - BBR_RS12535 0.46 +0.6 568,604 - BBR_RS12535 0.48 -1.2 568,619 + BBR_RS12535 0.49 -0.5 568,619 + BBR_RS12535 0.49 -0.3 568,634 - BBR_RS12535 0.50 +1.5 568,638 + BBR_RS12535 0.50 -0.1 568,648 - BBR_RS12535 0.51 +0.1 568,692 - BBR_RS12535 0.54 +1.4 568,696 + BBR_RS12535 0.55 -0.3 568,714 + BBR_RS12535 0.56 +0.2 568,716 + BBR_RS12535 0.56 -0.1 568,717 + BBR_RS12535 0.56 -0.1 568,736 + BBR_RS12535 0.58 -0.8 568,762 - BBR_RS12535 0.60 +0.2 568,762 - BBR_RS12535 0.60 +0.3 568,786 - BBR_RS12535 0.61 -0.3 568,827 - BBR_RS12535 0.64 -1.5 568,842 + BBR_RS12535 0.66 +0.5 568,898 + BBR_RS12535 0.70 -0.6 568,965 - BBR_RS12535 0.75 +0.1 569,004 + BBR_RS12535 0.78 +0.6 569,024 + BBR_RS12535 0.79 +0.1 569,032 - BBR_RS12535 0.80 +1.2 569,033 - BBR_RS12535 0.80 -2.1 569,035 + BBR_RS12535 0.80 -0.3 569,038 - BBR_RS12535 0.80 +0.0 569,038 - BBR_RS12535 0.80 -0.1 569,053 + BBR_RS12535 0.82 +0.2 569,077 - BBR_RS12535 0.83 -0.6 569,077 - BBR_RS12535 0.83 -0.7 569,113 + BBR_RS12535 0.86 +0.8 569,130 + BBR_RS12535 0.87 -0.3 569,130 + BBR_RS12535 0.87 -0.0 569,143 + BBR_RS12535 0.88 -0.1 569,151 - BBR_RS12535 0.89 +0.6 569,189 + +0.2 569,189 - +0.4 569,189 - -0.3 569,228 - +0.3 569,272 - +0.5 569,277 + -0.3 569,279 - -1.3 569,279 - -0.3 569,285 - +0.1 569,313 + +0.5 569,343 + +0.0 569,353 - -0.5 569,353 - -0.8 569,353 - -0.1 569,392 - -0.2 569,406 - -1.2 569,411 - -0.6 569,463 + -0.1 569,463 + +0.5 569,463 + +1.1 569,463 + -0.4 569,463 - +0.4 569,471 - -1.0 569,471 - +0.2 569,481 - +0.7 569,505 - BBR_RS12540 0.10 -0.9 569,541 + BBR_RS12540 0.13 +0.4 569,571 - BBR_RS12540 0.15 -0.4 569,606 + BBR_RS12540 0.17 -1.4 569,608 + BBR_RS12540 0.17 +1.1 569,645 - BBR_RS12540 0.19 -1.6 569,645 - BBR_RS12540 0.19 +0.3 569,775 + BBR_RS12540 0.27 +0.2 569,775 - BBR_RS12540 0.27 +0.4 569,868 + BBR_RS12540 0.33 -1.5 569,876 - BBR_RS12540 0.34 -0.4 569,897 - BBR_RS12540 0.35 -0.3 569,955 + BBR_RS12540 0.39 +0.5 569,955 + BBR_RS12540 0.39 +0.4 570,003 + BBR_RS12540 0.42 +0.5 570,013 - BBR_RS12540 0.42 +0.6 570,013 - BBR_RS12540 0.42 +0.2 570,035 - BBR_RS12540 0.44 -0.0 570,035 - BBR_RS12540 0.44 +0.2 570,042 + BBR_RS12540 0.44 +1.5 570,047 + BBR_RS12540 0.44 +1.1 570,055 - BBR_RS12540 0.45 -0.1 570,059 + BBR_RS12540 0.45 +2.2 570,108 - BBR_RS12540 0.48 -0.4 570,143 + BBR_RS12540 0.50 -0.1 570,143 + BBR_RS12540 0.50 +0.2 570,151 - BBR_RS12540 0.51 -1.4 570,151 - BBR_RS12540 0.51 +1.7 570,162 - BBR_RS12540 0.52 +0.5 570,162 - BBR_RS12540 0.52 -0.1 570,168 + BBR_RS12540 0.52 -0.9 570,176 - BBR_RS12540 0.52 +0.8 570,176 - BBR_RS12540 0.52 -0.2 570,176 - BBR_RS12540 0.52 -0.2 570,176 - BBR_RS12540 0.52 +0.1 570,176 - BBR_RS12540 0.52 +0.3 570,217 + BBR_RS12540 0.55 +0.7 570,260 - BBR_RS12540 0.58 -0.1 570,260 - BBR_RS12540 0.58 +0.4 570,260 - BBR_RS12540 0.58 -0.9 570,260 - BBR_RS12540 0.58 +1.5 570,308 + BBR_RS12540 0.61 +0.4 570,319 - BBR_RS12540 0.61 +0.6 570,341 - BBR_RS12540 0.63 +0.1 570,350 + BBR_RS12540 0.63 +0.5 570,423 + BBR_RS12540 0.68 +1.9 570,423 + BBR_RS12540 0.68 +1.0 570,437 + BBR_RS12540 0.69 +1.0 570,437 + BBR_RS12540 0.69 +1.0 570,445 - BBR_RS12540 0.69 -0.1 570,445 - BBR_RS12540 0.69 -0.1 570,445 - BBR_RS12540 0.69 +0.3 570,446 + BBR_RS12540 0.69 +0.5 570,487 - BBR_RS12540 0.72 -0.3 570,502 + BBR_RS12540 0.73 +0.3 570,507 - BBR_RS12540 0.73 +0.7 570,512 + BBR_RS12540 0.73 +0.1 570,512 + BBR_RS12540 0.73 +0.7 570,512 + BBR_RS12540 0.73 -2.3 570,515 - BBR_RS12540 0.74 +0.0 570,517 + BBR_RS12540 0.74 -0.3 570,525 - BBR_RS12540 0.74 +0.6 570,525 - BBR_RS12540 0.74 -1.0 570,548 + BBR_RS12540 0.76 -1.3 570,571 + BBR_RS12540 0.77 +0.7 570,576 + BBR_RS12540 0.77 -1.5 570,576 + BBR_RS12540 0.77 +0.7 570,686 + BBR_RS12540 0.84 +2.2 570,695 + BBR_RS12540 0.85 +0.2 570,699 - BBR_RS12540 0.85 +0.5 570,720 + BBR_RS12540 0.86 +1.2 570,720 + BBR_RS12540 0.86 +0.0 570,837 + +0.4 570,853 - +0.9 570,880 + +0.1 570,881 - +0.1 570,888 - -0.1 570,888 - +0.7 570,888 - +0.3 570,919 + +0.6 570,940 + +0.8 570,940 - +0.4 570,940 - -0.1 570,946 - +0.4 571,007 - -1.1 571,060 + +0.1 571,060 - -0.3 571,088 + +0.9 571,088 + +0.1 571,088 + +0.4 571,096 - -0.3 571,102 + +0.8 571,102 + +0.3 571,102 + +0.8 571,102 + +0.8 571,102 + +1.2 571,103 + +0.3 571,103 - +0.2 571,103 - +0.3 571,110 - +0.6 571,110 - +1.5 571,110 - +0.3 571,110 - +0.8 571,110 - -0.2 571,110 - +0.3 571,110 - +0.7 571,122 + +1.3 571,131 + +0.0 571,168 - -0.6 571,193 - -4.8 571,211 - -1.4 571,211 - -3.8 571,211 - -3.1 571,211 - -1.1 571,464 + -1.4 571,464 - +0.1 571,464 - -0.4 571,500 + BBR_RS12545 0.11 +0.8 571,534 + BBR_RS12545 0.13 -2.7 571,615 + BBR_RS12545 0.18 +0.1 571,783 + BBR_RS12545 0.27 -2.1 571,813 + BBR_RS12545 0.29 +0.4 571,923 + BBR_RS12545 0.35 -3.3
Or see this region's nucleotide sequence