Experiment: Recovery broth with Amoxicillin 0.003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12470 and BBR_RS12475 are separated by 347 nucleotides BBR_RS12475 and BBR_RS12480 are separated by 33 nucleotides
BBR_RS12470: BBR_RS12470 - long-chain fatty acid--CoA ligase, at 546,438 to 548,261
_RS12470
BBR_RS12475: BBR_RS12475 - heme utilization protein HutZ, at 548,609 to 548,875
_RS12475
BBR_RS12480: BBR_RS12480 - arabinose-proton symporter, at 548,909 to 549,109
_RS12480
Position (kb)
548
549 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 547.658 kb on + strand, within BBR_RS12470 at 547.661 kb on - strand, within BBR_RS12470 at 547.666 kb on + strand, within BBR_RS12470 at 547.666 kb on + strand, within BBR_RS12470 at 547.666 kb on - strand, within BBR_RS12470 at 547.666 kb on - strand, within BBR_RS12470 at 547.666 kb on - strand, within BBR_RS12470 at 547.674 kb on - strand, within BBR_RS12470 at 547.685 kb on + strand, within BBR_RS12470 at 547.693 kb on - strand, within BBR_RS12470 at 547.693 kb on - strand, within BBR_RS12470 at 547.698 kb on - strand, within BBR_RS12470 at 547.698 kb on - strand, within BBR_RS12470 at 547.699 kb on - strand, within BBR_RS12470 at 547.700 kb on - strand, within BBR_RS12470 at 547.700 kb on - strand, within BBR_RS12470 at 547.700 kb on - strand, within BBR_RS12470 at 547.700 kb on - strand, within BBR_RS12470 at 547.701 kb on - strand, within BBR_RS12470 at 547.746 kb on - strand, within BBR_RS12470 at 547.777 kb on - strand, within BBR_RS12470 at 547.787 kb on - strand, within BBR_RS12470 at 547.831 kb on + strand, within BBR_RS12470 at 547.839 kb on - strand, within BBR_RS12470 at 547.839 kb on - strand, within BBR_RS12470 at 547.931 kb on - strand, within BBR_RS12470 at 547.976 kb on - strand, within BBR_RS12470 at 547.990 kb on - strand, within BBR_RS12470 at 548.011 kb on - strand, within BBR_RS12470 at 548.012 kb on + strand, within BBR_RS12470 at 548.020 kb on - strand, within BBR_RS12470 at 548.025 kb on - strand, within BBR_RS12470 at 548.027 kb on - strand, within BBR_RS12470 at 548.033 kb on - strand, within BBR_RS12470 at 548.033 kb on - strand, within BBR_RS12470 at 548.047 kb on - strand, within BBR_RS12470 at 548.050 kb on - strand, within BBR_RS12470 at 548.067 kb on - strand, within BBR_RS12470 at 548.074 kb on - strand, within BBR_RS12470 at 548.192 kb on + strand at 548.197 kb on + strand at 548.203 kb on - strand at 548.266 kb on - strand at 548.266 kb on - strand at 548.266 kb on - strand at 548.266 kb on - strand at 548.324 kb on + strand at 548.332 kb on - strand at 548.332 kb on - strand at 548.334 kb on + strand at 548.353 kb on - strand at 548.360 kb on + strand at 548.389 kb on + strand at 548.392 kb on + strand at 548.394 kb on + strand at 548.406 kb on + strand at 548.406 kb on + strand at 548.406 kb on + strand at 548.406 kb on + strand at 548.406 kb on + strand at 548.414 kb on - strand at 548.425 kb on - strand at 548.431 kb on + strand at 548.436 kb on + strand at 548.439 kb on - strand at 548.441 kb on + strand at 548.441 kb on + strand at 548.441 kb on + strand at 548.441 kb on + strand at 548.449 kb on - strand at 548.449 kb on - strand at 548.449 kb on - strand at 548.449 kb on - strand at 548.483 kb on + strand at 548.483 kb on + strand at 548.483 kb on + strand at 548.485 kb on + strand at 548.485 kb on + strand at 548.491 kb on - strand at 548.491 kb on - strand at 548.513 kb on + strand at 548.534 kb on + strand at 548.565 kb on + strand at 548.565 kb on + strand at 548.569 kb on + strand at 548.577 kb on + strand at 548.586 kb on - strand at 548.611 kb on + strand at 548.613 kb on + strand at 548.639 kb on - strand, within BBR_RS12475 at 548.690 kb on + strand, within BBR_RS12475 at 548.692 kb on - strand, within BBR_RS12475 at 548.709 kb on - strand, within BBR_RS12475 at 548.709 kb on - strand, within BBR_RS12475 at 548.709 kb on - strand, within BBR_RS12475 at 548.725 kb on + strand, within BBR_RS12475 at 548.735 kb on - strand, within BBR_RS12475 at 548.766 kb on + strand, within BBR_RS12475 at 548.778 kb on + strand, within BBR_RS12475 at 548.790 kb on + strand, within BBR_RS12475 at 548.790 kb on + strand, within BBR_RS12475 at 548.796 kb on - strand, within BBR_RS12475 at 548.811 kb on + strand, within BBR_RS12475 at 548.811 kb on + strand, within BBR_RS12475 at 548.819 kb on - strand, within BBR_RS12475 at 548.821 kb on + strand, within BBR_RS12475 at 548.849 kb on + strand at 548.876 kb on + strand at 548.904 kb on + strand at 548.907 kb on - strand at 548.912 kb on - strand at 548.917 kb on - strand at 548.963 kb on + strand, within BBR_RS12480 at 548.963 kb on + strand, within BBR_RS12480 at 548.963 kb on + strand, within BBR_RS12480 at 548.966 kb on - strand, within BBR_RS12480 at 548.968 kb on + strand, within BBR_RS12480 at 548.968 kb on + strand, within BBR_RS12480 at 548.976 kb on + strand, within BBR_RS12480 at 548.976 kb on + strand, within BBR_RS12480 at 549.003 kb on + strand, within BBR_RS12480 at 549.018 kb on + strand, within BBR_RS12480 at 549.018 kb on - strand, within BBR_RS12480 at 549.046 kb on + strand, within BBR_RS12480 at 549.076 kb on + strand, within BBR_RS12480 at 549.076 kb on + strand, within BBR_RS12480 at 549.084 kb on - strand, within BBR_RS12480 at 549.084 kb on - strand, within BBR_RS12480 at 549.102 kb on + strand at 549.102 kb on + strand at 549.102 kb on + strand at 549.103 kb on + strand at 549.110 kb on - strand at 549.110 kb on - strand at 549.110 kb on - strand at 549.110 kb on - strand at 549.110 kb on - strand at 549.112 kb on + strand at 549.132 kb on - strand at 549.151 kb on - strand at 549.151 kb on - strand at 549.153 kb on + strand at 549.163 kb on + strand at 549.163 kb on + strand at 549.165 kb on + strand at 549.166 kb on - strand at 549.204 kb on + strand at 549.217 kb on - strand at 549.225 kb on - strand at 549.230 kb on - strand at 549.241 kb on - strand at 549.254 kb on - strand at 549.254 kb on - strand at 549.256 kb on + strand at 549.351 kb on - strand at 549.427 kb on + strand at 549.435 kb on - strand at 549.454 kb on - strand at 549.457 kb on + strand at 549.477 kb on + strand at 549.512 kb on + strand at 549.520 kb on - strand at 549.520 kb on - strand at 549.572 kb on + strand at 549.580 kb on - strand at 549.580 kb on - strand at 549.584 kb on + strand at 549.614 kb on + strand at 549.636 kb on - strand at 549.669 kb on + strand at 549.677 kb on + strand at 549.692 kb on + strand at 549.698 kb on + strand at 549.702 kb on - strand at 549.760 kb on + strand at 549.780 kb on - strand at 549.790 kb on - strand at 549.819 kb on + strand at 549.819 kb on + strand at 549.865 kb on + strand at 549.865 kb on + strand at 549.866 kb on + strand at 549.873 kb on - strand at 549.873 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Amoxicillin 0.003 mM remove 547,658 + BBR_RS12470 0.67 -0.4 547,661 - BBR_RS12470 0.67 +0.7 547,666 + BBR_RS12470 0.67 -0.4 547,666 + BBR_RS12470 0.67 -1.6 547,666 - BBR_RS12470 0.67 -0.2 547,666 - BBR_RS12470 0.67 +0.5 547,666 - BBR_RS12470 0.67 +0.3 547,674 - BBR_RS12470 0.68 +0.0 547,685 + BBR_RS12470 0.68 +0.2 547,693 - BBR_RS12470 0.69 +1.5 547,693 - BBR_RS12470 0.69 +1.0 547,698 - BBR_RS12470 0.69 +0.3 547,698 - BBR_RS12470 0.69 +0.7 547,699 - BBR_RS12470 0.69 +0.4 547,700 - BBR_RS12470 0.69 +1.3 547,700 - BBR_RS12470 0.69 +1.0 547,700 - BBR_RS12470 0.69 +0.1 547,700 - BBR_RS12470 0.69 -0.4 547,701 - BBR_RS12470 0.69 -0.2 547,746 - BBR_RS12470 0.72 +0.8 547,777 - BBR_RS12470 0.73 +0.3 547,787 - BBR_RS12470 0.74 +0.4 547,831 + BBR_RS12470 0.76 -0.5 547,839 - BBR_RS12470 0.77 +0.3 547,839 - BBR_RS12470 0.77 +0.8 547,931 - BBR_RS12470 0.82 +0.8 547,976 - BBR_RS12470 0.84 -0.9 547,990 - BBR_RS12470 0.85 +0.5 548,011 - BBR_RS12470 0.86 +0.3 548,012 + BBR_RS12470 0.86 +0.5 548,020 - BBR_RS12470 0.87 +0.1 548,025 - BBR_RS12470 0.87 +0.9 548,027 - BBR_RS12470 0.87 -0.6 548,033 - BBR_RS12470 0.87 -0.0 548,033 - BBR_RS12470 0.87 -0.1 548,047 - BBR_RS12470 0.88 +0.6 548,050 - BBR_RS12470 0.88 -0.2 548,067 - BBR_RS12470 0.89 -0.1 548,074 - BBR_RS12470 0.90 +0.2 548,192 + +1.3 548,197 + +1.6 548,203 - +0.9 548,266 - +0.2 548,266 - +0.1 548,266 - +0.1 548,266 - +0.2 548,324 + -0.1 548,332 - +0.2 548,332 - +0.5 548,334 + +0.3 548,353 - -0.6 548,360 + -0.8 548,389 + +0.4 548,392 + -0.2 548,394 + -0.2 548,406 + +0.3 548,406 + -0.2 548,406 + -0.9 548,406 + +0.4 548,406 + +0.7 548,414 - -0.9 548,425 - -0.2 548,431 + +0.3 548,436 + +0.9 548,439 - -1.8 548,441 + -0.2 548,441 + +0.7 548,441 + -0.0 548,441 + -0.4 548,449 - -0.3 548,449 - -0.0 548,449 - +0.4 548,449 - +0.4 548,483 + +0.3 548,483 + -0.8 548,483 + +0.4 548,485 + -0.2 548,485 + -0.7 548,491 - +0.1 548,491 - -1.8 548,513 + -1.7 548,534 + -0.4 548,565 + +0.1 548,565 + -0.0 548,569 + -0.0 548,577 + +0.0 548,586 - -0.1 548,611 + +0.1 548,613 + +0.4 548,639 - BBR_RS12475 0.11 -0.5 548,690 + BBR_RS12475 0.30 +0.5 548,692 - BBR_RS12475 0.31 -0.5 548,709 - BBR_RS12475 0.37 +0.1 548,709 - BBR_RS12475 0.37 -1.9 548,709 - BBR_RS12475 0.37 +0.7 548,725 + BBR_RS12475 0.43 +0.5 548,735 - BBR_RS12475 0.47 +0.4 548,766 + BBR_RS12475 0.59 +0.7 548,778 + BBR_RS12475 0.63 +0.7 548,790 + BBR_RS12475 0.68 +1.8 548,790 + BBR_RS12475 0.68 -0.4 548,796 - BBR_RS12475 0.70 +1.6 548,811 + BBR_RS12475 0.76 -0.1 548,811 + BBR_RS12475 0.76 -0.2 548,819 - BBR_RS12475 0.79 +0.7 548,821 + BBR_RS12475 0.79 -0.2 548,849 + -0.2 548,876 + -0.3 548,904 + -1.4 548,907 - +0.4 548,912 - -0.3 548,917 - +0.1 548,963 + BBR_RS12480 0.27 +0.2 548,963 + BBR_RS12480 0.27 -0.0 548,963 + BBR_RS12480 0.27 -0.0 548,966 - BBR_RS12480 0.28 +0.1 548,968 + BBR_RS12480 0.29 -0.5 548,968 + BBR_RS12480 0.29 -0.3 548,976 + BBR_RS12480 0.33 +0.7 548,976 + BBR_RS12480 0.33 +0.2 549,003 + BBR_RS12480 0.47 +1.0 549,018 + BBR_RS12480 0.54 -1.4 549,018 - BBR_RS12480 0.54 +0.7 549,046 + BBR_RS12480 0.68 -0.3 549,076 + BBR_RS12480 0.83 -0.3 549,076 + BBR_RS12480 0.83 +0.2 549,084 - BBR_RS12480 0.87 +0.2 549,084 - BBR_RS12480 0.87 +0.6 549,102 + +0.1 549,102 + +0.2 549,102 + +0.3 549,103 + -0.1 549,110 - -0.4 549,110 - -0.2 549,110 - +0.4 549,110 - +0.4 549,110 - +0.3 549,112 + +0.6 549,132 - +0.4 549,151 - +0.6 549,151 - +0.7 549,153 + -0.8 549,163 + +0.3 549,163 + -1.1 549,165 + +0.2 549,166 - -0.5 549,204 + -0.0 549,217 - -0.2 549,225 - +1.1 549,230 - +1.2 549,241 - -0.9 549,254 - -0.3 549,254 - +0.3 549,256 + -0.5 549,351 - -1.0 549,427 + +0.0 549,435 - -0.1 549,454 - +0.6 549,457 + +0.4 549,477 + +0.8 549,512 + +0.2 549,520 - +0.3 549,520 - -0.3 549,572 + -0.3 549,580 - +0.3 549,580 - -0.4 549,584 + +0.5 549,614 + +1.6 549,636 - +0.4 549,669 + -0.9 549,677 + -0.2 549,692 + -0.3 549,698 + -0.5 549,702 - +0.4 549,760 + -0.2 549,780 - +0.7 549,790 - -0.3 549,819 + +0.3 549,819 + +0.2 549,865 + -0.1 549,865 + -0.3 549,866 + +0.8 549,873 - +0.1 549,873 - -1.1
Or see this region's nucleotide sequence