Experiment: Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00010 and SMc00450 overlap by 4 nucleotides SMc00450 and SMc00130 overlap by 1 nucleotides SMc00130 and SMc00012 are separated by 11 nucleotides SMc00012 and SMc00013 are separated by 61 nucleotides
SMc00010: SMc00010 - cytochrome C oxidase polypeptide I transmembrane protein, at 992,942 to 994,630
SMc00010
SMc00450: SMc00450 - protoheme IX farnesyltransferase, at 994,627 to 995,679
SMc00450
SMc00130: SMc00130 - hypothetical protein, at 995,679 to 995,819
SMc00130
SMc00012: SMc00012 - cytochrome C oxidase assembly protein, at 995,831 to 996,427
SMc00012
SMc00013: SMc00013 - cytochrome C oxidase subunit III transmembrane protein, at 996,489 to 997,367
SMc00013
Position (kb) 994
995
996 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 994.200 kb on - strand, within SMc00010 at 994.220 kb on + strand, within SMc00010 at 994.298 kb on + strand, within SMc00010 at 994.722 kb on + strand at 995.143 kb on + strand, within SMc00450 at 995.155 kb on + strand, within SMc00450 at 995.420 kb on - strand, within SMc00450 at 995.927 kb on + strand, within SMc00012 at 995.972 kb on + strand, within SMc00012 at 996.321 kb on + strand, within SMc00012 at 996.472 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.520 kb on - strand at 996.607 kb on - strand, within SMc00013
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.001 mg/ml remove 994,200 - SMc00010 0.74 -1.3 994,220 + SMc00010 0.76 -3.5 994,298 + SMc00010 0.80 -2.4 994,722 + +0.1 995,143 + SMc00450 0.49 -2.0 995,155 + SMc00450 0.50 -2.4 995,420 - SMc00450 0.75 -0.4 995,927 + SMc00012 0.16 -0.7 995,972 + SMc00012 0.24 -1.9 996,321 + SMc00012 0.82 -2.2 996,472 + +1.6 996,519 + -1.9 996,519 + -1.5 996,519 + +0.1 996,519 + +2.3 996,519 + -1.0 996,520 - -2.2 996,607 - SMc00013 0.13 +0.6
Or see this region's nucleotide sequence