Experiment: Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1450 and SMa1452 are separated by 24 nucleotides SMa1452 and SMa1454 are separated by 121 nucleotides SMa1454 and SMa1455 overlap by 19 nucleotides SMa1455 and SMa1456 overlap by 4 nucleotides SMa1456 and SMa1459 are separated by 107 nucleotides
SMa1450: SMa1450 - thiolase, at 799,195 to 800,385
SMa1450
SMa1452: SMa1452 - hypothetical protein, at 800,410 to 801,177
SMa1452
SMa1454: SMa1454 - AraC family transcriptional regulator, at 801,299 to 802,342
SMa1454
SMa1455: SMa1455 - hypothetical protein, at 802,324 to 802,842
SMa1455
SMa1456: SMa1456 - hypothetical protein, at 802,839 to 803,228
SMa1456
SMa1459: SMa1459 - Dehydrogenase, malate/L-lactate family, at 803,336 to 804,340
SMa1459
Position (kb) 801
802
803 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 800.469 kb on + strand at 800.469 kb on + strand at 800.469 kb on + strand at 800.470 kb on - strand at 800.470 kb on - strand at 800.470 kb on - strand at 800.481 kb on + strand at 800.481 kb on + strand at 800.481 kb on + strand at 800.501 kb on + strand, within SMa1452 at 800.502 kb on - strand, within SMa1452 at 800.502 kb on - strand, within SMa1452 at 800.593 kb on - strand, within SMa1452 at 800.733 kb on + strand, within SMa1452 at 800.734 kb on - strand, within SMa1452 at 801.153 kb on + strand at 801.225 kb on - strand at 801.229 kb on + strand at 801.229 kb on + strand at 801.230 kb on - strand at 801.230 kb on - strand at 801.437 kb on + strand, within SMa1454 at 801.438 kb on - strand, within SMa1454 at 801.594 kb on + strand, within SMa1454 at 801.595 kb on - strand, within SMa1454 at 801.696 kb on - strand, within SMa1454 at 801.700 kb on + strand, within SMa1454 at 801.700 kb on + strand, within SMa1454 at 801.876 kb on - strand, within SMa1454 at 801.876 kb on - strand, within SMa1454 at 801.943 kb on + strand, within SMa1454 at 801.944 kb on - strand, within SMa1454 at 801.988 kb on - strand, within SMa1454 at 801.988 kb on - strand, within SMa1454 at 802.006 kb on + strand, within SMa1454 at 802.007 kb on - strand, within SMa1454 at 802.007 kb on - strand, within SMa1454 at 802.273 kb on + strand at 802.274 kb on - strand at 802.279 kb on + strand at 802.280 kb on - strand at 802.347 kb on + strand at 802.370 kb on + strand at 802.370 kb on + strand at 802.371 kb on - strand at 802.371 kb on - strand at 802.371 kb on - strand at 802.637 kb on + strand, within SMa1455 at 802.637 kb on + strand, within SMa1455 at 802.638 kb on - strand, within SMa1455 at 802.722 kb on - strand, within SMa1455 at 803.176 kb on + strand, within SMa1456 at 803.176 kb on + strand, within SMa1456 at 803.177 kb on - strand, within SMa1456 at 803.177 kb on - strand, within SMa1456 at 803.177 kb on - strand, within SMa1456 at 803.177 kb on - strand, within SMa1456 at 803.229 kb on - strand at 803.250 kb on + strand at 803.338 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.001 mg/ml remove 800,469 + -0.4 800,469 + +0.1 800,469 + -3.7 800,470 - -0.4 800,470 - -1.1 800,470 - +0.2 800,481 + +0.3 800,481 + -0.8 800,481 + +0.7 800,501 + SMa1452 0.12 -0.1 800,502 - SMa1452 0.12 -1.0 800,502 - SMa1452 0.12 -1.7 800,593 - SMa1452 0.24 -0.8 800,733 + SMa1452 0.42 -0.4 800,734 - SMa1452 0.42 +0.1 801,153 + +0.6 801,225 - +1.2 801,229 + -0.4 801,229 + +0.5 801,230 - -0.9 801,230 - -1.8 801,437 + SMa1454 0.13 +0.4 801,438 - SMa1454 0.13 -1.1 801,594 + SMa1454 0.28 +0.4 801,595 - SMa1454 0.28 +1.5 801,696 - SMa1454 0.38 +0.5 801,700 + SMa1454 0.38 +0.4 801,700 + SMa1454 0.38 +0.5 801,876 - SMa1454 0.55 -3.0 801,876 - SMa1454 0.55 +0.1 801,943 + SMa1454 0.62 +1.0 801,944 - SMa1454 0.62 +1.1 801,988 - SMa1454 0.66 +0.2 801,988 - SMa1454 0.66 -0.4 802,006 + SMa1454 0.68 +0.1 802,007 - SMa1454 0.68 +1.4 802,007 - SMa1454 0.68 +0.8 802,273 + -0.0 802,274 - -1.1 802,279 + +0.5 802,280 - +0.2 802,347 + -0.1 802,370 + -0.7 802,370 + +1.5 802,371 - -2.2 802,371 - -0.3 802,371 - +1.2 802,637 + SMa1455 0.60 -0.2 802,637 + SMa1455 0.60 +1.4 802,638 - SMa1455 0.61 +0.3 802,722 - SMa1455 0.77 -1.2 803,176 + SMa1456 0.86 +0.5 803,176 + SMa1456 0.86 +0.4 803,177 - SMa1456 0.87 -0.3 803,177 - SMa1456 0.87 +0.2 803,177 - SMa1456 0.87 -0.3 803,177 - SMa1456 0.87 -3.7 803,229 - -1.7 803,250 + -1.4 803,338 - -0.7
Or see this region's nucleotide sequence