Experiment: Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0007 and SMa0009 are separated by 38 nucleotides SMa0009 and SMa0011 are separated by 4 nucleotides SMa0011 and SMa0013 overlap by 92 nucleotides SMa0013 and SMa0015 overlap by 4 nucleotides
SMa0007: SMa0007 - formate dehydrogenase subunit gamma, at 5,424 to 6,110
SMa0007
SMa0009: SMa0009 - formate dehydrogenase subunit epsilon, at 6,149 to 7,069
SMa0009
SMa0011: SMa0011 - selenocysteine synthase, at 7,074 to 8,474
SMa0011
SMa0013: SMa0013 - hypothetical protein, at 8,383 to 9,255
SMa0013
SMa0015: SMa0015 - selenocysteine-specific elongation factor, at 9,252 to 11,252
SMa0015
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6.166 kb on - strand at 6.296 kb on + strand, within SMa0009 at 6.297 kb on - strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.620 kb on + strand, within SMa0009 at 6.621 kb on - strand, within SMa0009 at 6.621 kb on - strand, within SMa0009 at 6.621 kb on - strand, within SMa0009 at 6.861 kb on - strand, within SMa0009 at 6.944 kb on + strand, within SMa0009 at 7.028 kb on + strand at 7.028 kb on + strand at 7.196 kb on - strand at 7.340 kb on + strand, within SMa0011 at 7.341 kb on - strand, within SMa0011 at 7.695 kb on + strand, within SMa0011 at 7.696 kb on - strand, within SMa0011 at 7.696 kb on - strand, within SMa0011 at 7.742 kb on + strand, within SMa0011 at 7.743 kb on - strand, within SMa0011 at 7.752 kb on + strand, within SMa0011 at 7.872 kb on + strand, within SMa0011 at 7.873 kb on - strand, within SMa0011 at 8.086 kb on - strand, within SMa0011 at 8.249 kb on - strand, within SMa0011 at 8.490 kb on + strand, within SMa0013 at 8.491 kb on - strand at 8.491 kb on - strand, within SMa0013 at 8.491 kb on - strand, within SMa0013 at 8.558 kb on - strand, within SMa0013 at 8.731 kb on + strand, within SMa0013 at 9.007 kb on + strand, within SMa0013 at 9.007 kb on + strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.008 kb on - strand, within SMa0013 at 9.075 kb on + strand, within SMa0013 at 9.382 kb on - strand at 9.449 kb on + strand at 9.449 kb on + strand at 9.449 kb on + strand at 9.450 kb on - strand at 9.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.001 mg/ml remove 6,166 - +0.1 6,296 + SMa0009 0.16 +0.8 6,297 - SMa0009 0.16 +0.2 6,620 + SMa0009 0.51 -1.0 6,620 + SMa0009 0.51 -2.0 6,620 + SMa0009 0.51 +0.2 6,620 + SMa0009 0.51 +0.2 6,621 - SMa0009 0.51 +1.1 6,621 - SMa0009 0.51 -0.3 6,621 - SMa0009 0.51 -1.7 6,861 - SMa0009 0.77 +0.6 6,944 + SMa0009 0.86 +0.7 7,028 + -0.0 7,028 + -2.4 7,196 - -0.1 7,340 + SMa0011 0.19 -0.8 7,341 - SMa0011 0.19 -0.3 7,695 + SMa0011 0.44 -0.2 7,696 - SMa0011 0.44 +0.0 7,696 - SMa0011 0.44 +1.6 7,742 + SMa0011 0.48 +0.9 7,743 - SMa0011 0.48 +0.3 7,752 + SMa0011 0.48 -1.2 7,872 + SMa0011 0.57 -1.6 7,873 - SMa0011 0.57 -0.5 8,086 - SMa0011 0.72 -2.2 8,249 - SMa0011 0.84 -1.7 8,490 + SMa0013 0.12 +0.4 8,491 - +0.3 8,491 - SMa0013 0.12 -1.0 8,491 - SMa0013 0.12 -0.3 8,558 - SMa0013 0.20 +0.3 8,731 + SMa0013 0.40 +0.1 9,007 + SMa0013 0.71 +0.1 9,007 + SMa0013 0.71 -0.2 9,008 - SMa0013 0.72 +0.8 9,008 - SMa0013 0.72 -2.7 9,008 - SMa0013 0.72 +0.0 9,008 - SMa0013 0.72 +0.8 9,075 + SMa0013 0.79 +0.0 9,382 - -0.1 9,449 + +0.1 9,449 + +0.3 9,449 + +2.6 9,450 - -1.3 9,450 - -0.5
Or see this region's nucleotide sequence