Experiment: Cisplatin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21481 and SM_b21482 are separated by 296 nucleotides SM_b21482 and SM_b21483 are separated by 92 nucleotides SM_b21483 and SM_b21686 are separated by 16 nucleotides SM_b21686 and SM_b21484 are separated by 38 nucleotides SM_b21484 and SM_b21687 are separated by 65 nucleotides SM_b21687 and SM_b21485 are separated by 142 nucleotides
SM_b21481: SM_b21481 - hypothetical protein, at 1,403,628 to 1,404,104
_b21481
SM_b21482: SM_b21482 - hypothetical protein, at 1,404,401 to 1,404,703
_b21482
SM_b21483: SM_b21483 - hypothetical protein, at 1,404,796 to 1,405,077
_b21483
SM_b21686: SM_b21686 - hypothetical protein, at 1,405,094 to 1,405,279
_b21686
SM_b21484: SM_b21484 - RNA polymerase sigma-E factor (sigma-24) protein, at 1,405,318 to 1,405,839
_b21484
SM_b21687: SM_b21687 - hypothetical protein, at 1,405,905 to 1,406,099
_b21687
SM_b21485: SM_b21485 - hypothetical protein, at 1,406,242 to 1,406,682
_b21485
Position (kb)
1405
1406 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1404.094 kb on + strand at 1404.095 kb on - strand at 1404.195 kb on + strand at 1404.195 kb on + strand at 1404.196 kb on - strand at 1404.297 kb on + strand at 1404.298 kb on - strand at 1404.298 kb on - strand at 1404.352 kb on - strand at 1404.364 kb on + strand at 1404.602 kb on + strand, within SM_b21482 at 1404.603 kb on - strand, within SM_b21482 at 1404.722 kb on - strand at 1404.722 kb on - strand at 1404.765 kb on - strand at 1404.765 kb on - strand at 1404.849 kb on + strand, within SM_b21483 at 1404.866 kb on + strand, within SM_b21483 at 1404.866 kb on + strand, within SM_b21483 at 1404.867 kb on - strand, within SM_b21483 at 1404.948 kb on - strand, within SM_b21483 at 1404.948 kb on - strand, within SM_b21483 at 1404.948 kb on - strand, within SM_b21483 at 1404.954 kb on + strand, within SM_b21483 at 1404.955 kb on - strand, within SM_b21483 at 1404.976 kb on + strand, within SM_b21483 at 1404.976 kb on + strand, within SM_b21483 at 1404.977 kb on - strand, within SM_b21483 at 1404.977 kb on - strand, within SM_b21483 at 1404.980 kb on + strand, within SM_b21483 at 1404.980 kb on + strand, within SM_b21483 at 1404.988 kb on + strand, within SM_b21483 at 1404.989 kb on - strand, within SM_b21483 at 1404.989 kb on - strand, within SM_b21483 at 1405.059 kb on + strand at 1405.059 kb on + strand at 1405.172 kb on + strand, within SM_b21686 at 1405.173 kb on - strand, within SM_b21686 at 1405.192 kb on + strand, within SM_b21686 at 1405.193 kb on - strand, within SM_b21686 at 1405.296 kb on - strand at 1405.296 kb on - strand at 1405.311 kb on - strand at 1405.555 kb on + strand, within SM_b21484 at 1405.556 kb on - strand, within SM_b21484 at 1405.556 kb on - strand, within SM_b21484 at 1405.556 kb on - strand, within SM_b21484 at 1405.556 kb on - strand, within SM_b21484 at 1405.556 kb on - strand, within SM_b21484 at 1405.655 kb on + strand, within SM_b21484 at 1405.656 kb on - strand, within SM_b21484 at 1405.656 kb on - strand, within SM_b21484 at 1405.729 kb on + strand, within SM_b21484 at 1405.729 kb on + strand, within SM_b21484 at 1405.730 kb on - strand, within SM_b21484 at 1405.730 kb on - strand, within SM_b21484 at 1405.730 kb on - strand, within SM_b21484 at 1405.731 kb on + strand, within SM_b21484 at 1405.732 kb on - strand, within SM_b21484 at 1405.732 kb on - strand, within SM_b21484 at 1405.748 kb on + strand, within SM_b21484 at 1405.748 kb on + strand at 1405.749 kb on - strand, within SM_b21484 at 1405.749 kb on - strand, within SM_b21484 at 1405.767 kb on + strand, within SM_b21484 at 1406.028 kb on - strand, within SM_b21687 at 1406.028 kb on - strand, within SM_b21687 at 1406.077 kb on - strand, within SM_b21687 at 1406.090 kb on - strand at 1406.098 kb on - strand at 1406.129 kb on - strand at 1406.186 kb on - strand at 1406.251 kb on + strand at 1406.252 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.001 mg/ml remove 1,404,094 + -0.8 1,404,095 - -0.1 1,404,195 + +0.7 1,404,195 + +1.8 1,404,196 - +0.2 1,404,297 + -2.7 1,404,298 - +2.0 1,404,298 - +0.0 1,404,352 - -0.8 1,404,364 + -2.5 1,404,602 + SM_b21482 0.66 -1.2 1,404,603 - SM_b21482 0.67 +0.0 1,404,722 - +0.6 1,404,722 - -1.2 1,404,765 - +0.6 1,404,765 - -0.0 1,404,849 + SM_b21483 0.19 -0.6 1,404,866 + SM_b21483 0.25 +0.1 1,404,866 + SM_b21483 0.25 -1.1 1,404,867 - SM_b21483 0.25 +0.2 1,404,948 - SM_b21483 0.54 -1.7 1,404,948 - SM_b21483 0.54 -0.1 1,404,948 - SM_b21483 0.54 -0.1 1,404,954 + SM_b21483 0.56 -1.6 1,404,955 - SM_b21483 0.56 +0.3 1,404,976 + SM_b21483 0.64 -0.2 1,404,976 + SM_b21483 0.64 -2.8 1,404,977 - SM_b21483 0.64 -1.2 1,404,977 - SM_b21483 0.64 +0.9 1,404,980 + SM_b21483 0.65 -2.2 1,404,980 + SM_b21483 0.65 +0.9 1,404,988 + SM_b21483 0.68 +0.3 1,404,989 - SM_b21483 0.68 -1.6 1,404,989 - SM_b21483 0.68 +0.9 1,405,059 + +0.1 1,405,059 + +0.0 1,405,172 + SM_b21686 0.42 -1.0 1,405,173 - SM_b21686 0.42 -0.3 1,405,192 + SM_b21686 0.53 -0.9 1,405,193 - SM_b21686 0.53 +0.1 1,405,296 - +3.1 1,405,296 - +0.6 1,405,311 - -0.3 1,405,555 + SM_b21484 0.45 +3.2 1,405,556 - SM_b21484 0.46 +0.4 1,405,556 - SM_b21484 0.46 -0.5 1,405,556 - SM_b21484 0.46 -0.9 1,405,556 - SM_b21484 0.46 +0.2 1,405,556 - SM_b21484 0.46 +0.4 1,405,655 + SM_b21484 0.65 -0.3 1,405,656 - SM_b21484 0.65 -0.6 1,405,656 - SM_b21484 0.65 -3.1 1,405,729 + SM_b21484 0.79 -2.3 1,405,729 + SM_b21484 0.79 -1.1 1,405,730 - SM_b21484 0.79 +2.2 1,405,730 - SM_b21484 0.79 -0.5 1,405,730 - SM_b21484 0.79 +0.2 1,405,731 + SM_b21484 0.79 -1.0 1,405,732 - SM_b21484 0.79 -2.2 1,405,732 - SM_b21484 0.79 -1.1 1,405,748 + SM_b21484 0.82 -1.9 1,405,748 + +1.2 1,405,749 - SM_b21484 0.83 +0.7 1,405,749 - SM_b21484 0.83 +0.4 1,405,767 + SM_b21484 0.86 -3.0 1,406,028 - SM_b21687 0.63 +1.5 1,406,028 - SM_b21687 0.63 +0.0 1,406,077 - SM_b21687 0.88 -3.1 1,406,090 - -0.1 1,406,098 - +1.3 1,406,129 - -0.0 1,406,186 - -0.3 1,406,251 + +0.2 1,406,252 - +0.7
Or see this region's nucleotide sequence