Experiment: Cisplatin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2287 and SMa2289 are separated by 140 nucleotides SMa2289 and SMa2291 are separated by 1 nucleotides SMa2291 and SMa2293 are separated by 75 nucleotides
SMa2287: SMa2287 - LysR family transcriptional regulator, at 1,271,938 to 1,272,870
SMa2287
SMa2289: SMa2289 - hypothetical protein, at 1,273,011 to 1,273,403
SMa2289
SMa2291: SMa2291 - dehydrogenase, at 1,273,405 to 1,274,517
SMa2291
SMa2293: SMa2293 - beta lactamase transcriptional activator, at 1,274,593 to 1,275,477
SMa2293
Position (kb)
1273
1274
1275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1272.423 kb on - strand, within SMa2287 at 1272.516 kb on - strand, within SMa2287 at 1272.889 kb on + strand at 1272.890 kb on - strand at 1272.946 kb on + strand at 1272.946 kb on + strand at 1272.946 kb on + strand at 1272.947 kb on - strand at 1272.947 kb on - strand at 1272.947 kb on - strand at 1272.947 kb on - strand at 1272.947 kb on - strand at 1272.947 kb on - strand at 1272.947 kb on - strand at 1273.048 kb on - strand at 1273.279 kb on + strand, within SMa2289 at 1273.549 kb on + strand, within SMa2291 at 1273.552 kb on + strand, within SMa2291 at 1273.552 kb on + strand, within SMa2291 at 1273.553 kb on - strand, within SMa2291 at 1273.604 kb on - strand, within SMa2291 at 1273.604 kb on - strand, within SMa2291 at 1273.604 kb on - strand, within SMa2291 at 1273.618 kb on + strand, within SMa2291 at 1273.619 kb on - strand, within SMa2291 at 1273.631 kb on - strand, within SMa2291 at 1273.765 kb on + strand, within SMa2291 at 1273.996 kb on + strand, within SMa2291 at 1273.997 kb on - strand, within SMa2291 at 1273.997 kb on - strand, within SMa2291 at 1274.515 kb on + strand at 1274.515 kb on + strand at 1274.516 kb on - strand at 1274.518 kb on + strand at 1274.518 kb on + strand at 1274.571 kb on + strand at 1274.571 kb on + strand at 1274.571 kb on + strand at 1274.594 kb on + strand at 1274.594 kb on + strand at 1274.594 kb on + strand at 1274.596 kb on + strand at 1274.596 kb on + strand at 1274.597 kb on - strand at 1274.597 kb on - strand at 1274.597 kb on - strand at 1274.652 kb on + strand at 1274.694 kb on - strand, within SMa2293 at 1274.903 kb on + strand, within SMa2293 at 1274.904 kb on - strand, within SMa2293 at 1274.912 kb on - strand, within SMa2293 at 1274.912 kb on - strand, within SMa2293 at 1275.087 kb on + strand, within SMa2293 at 1275.087 kb on + strand, within SMa2293 at 1275.087 kb on + strand, within SMa2293 at 1275.088 kb on - strand, within SMa2293 at 1275.227 kb on - strand, within SMa2293 at 1275.251 kb on - strand, within SMa2293 at 1275.251 kb on - strand, within SMa2293 at 1275.262 kb on + strand, within SMa2293 at 1275.388 kb on + strand, within SMa2293
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0005 mg/ml remove 1,272,423 - SMa2287 0.52 +1.3 1,272,516 - SMa2287 0.62 +1.0 1,272,889 + +0.3 1,272,890 - -1.1 1,272,946 + -0.1 1,272,946 + -1.1 1,272,946 + +0.1 1,272,947 - +0.4 1,272,947 - -3.7 1,272,947 - +0.4 1,272,947 - -1.2 1,272,947 - +0.5 1,272,947 - -1.2 1,272,947 - -1.0 1,273,048 - -0.3 1,273,279 + SMa2289 0.68 -1.7 1,273,549 + SMa2291 0.13 +2.3 1,273,552 + SMa2291 0.13 -0.3 1,273,552 + SMa2291 0.13 -0.2 1,273,553 - SMa2291 0.13 -3.9 1,273,604 - SMa2291 0.18 -0.7 1,273,604 - SMa2291 0.18 +4.4 1,273,604 - SMa2291 0.18 -0.8 1,273,618 + SMa2291 0.19 -1.7 1,273,619 - SMa2291 0.19 +0.2 1,273,631 - SMa2291 0.20 -1.5 1,273,765 + SMa2291 0.32 -0.3 1,273,996 + SMa2291 0.53 -0.3 1,273,997 - SMa2291 0.53 -0.3 1,273,997 - SMa2291 0.53 -0.4 1,274,515 + -1.9 1,274,515 + -0.5 1,274,516 - +0.2 1,274,518 + +1.3 1,274,518 + -1.8 1,274,571 + +1.4 1,274,571 + +0.3 1,274,571 + -0.7 1,274,594 + -0.2 1,274,594 + -1.2 1,274,594 + +0.5 1,274,596 + -0.1 1,274,596 + -0.4 1,274,597 - +0.3 1,274,597 - -1.0 1,274,597 - -1.8 1,274,652 + -1.0 1,274,694 - SMa2293 0.11 -2.5 1,274,903 + SMa2293 0.35 +1.3 1,274,904 - SMa2293 0.35 -0.4 1,274,912 - SMa2293 0.36 -2.7 1,274,912 - SMa2293 0.36 -0.1 1,275,087 + SMa2293 0.56 +0.2 1,275,087 + SMa2293 0.56 -1.2 1,275,087 + SMa2293 0.56 -1.8 1,275,088 - SMa2293 0.56 +0.7 1,275,227 - SMa2293 0.72 +0.6 1,275,251 - SMa2293 0.74 -0.2 1,275,251 - SMa2293 0.74 +0.5 1,275,262 + SMa2293 0.76 +0.7 1,275,388 + SMa2293 0.90 -0.4
Or see this region's nucleotide sequence