Experiment: Cisplatin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1406 and SMa1408 are separated by 12 nucleotides SMa1408 and SMa1409 overlap by 4 nucleotides SMa1409 and SMa1410 are separated by 112 nucleotides
SMa1406: SMa1406 - TtuD3 hydroxypyruvate reductase, at 772,165 to 773,424
SMa1406
SMa1408: SMa1408 - dehydratase, at 773,437 to 774,207
SMa1408
SMa1409: SMa1409 - acyl-CoA transferase, at 774,204 to 775,775
SMa1409
SMa1410: SMa1410 - oxidoreductase, at 775,888 to 776,721
SMa1410
Position (kb) 774
775
776 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 773.210 kb on + strand, within SMa1406 at 773.210 kb on + strand, within SMa1406 at 773.216 kb on + strand, within SMa1406 at 773.583 kb on + strand, within SMa1408 at 773.583 kb on + strand, within SMa1408 at 773.615 kb on - strand, within SMa1408 at 773.615 kb on - strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.708 kb on + strand, within SMa1408 at 773.795 kb on - strand, within SMa1408 at 773.821 kb on + strand, within SMa1408 at 773.821 kb on + strand, within SMa1408 at 773.885 kb on - strand, within SMa1408 at 774.056 kb on - strand, within SMa1408 at 774.056 kb on - strand, within SMa1408 at 774.142 kb on - strand at 774.142 kb on - strand at 774.213 kb on + strand at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.388 kb on + strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.389 kb on - strand, within SMa1409 at 774.566 kb on + strand, within SMa1409 at 774.567 kb on - strand, within SMa1409 at 774.698 kb on - strand, within SMa1409 at 774.860 kb on + strand, within SMa1409 at 774.956 kb on + strand, within SMa1409 at 774.956 kb on + strand, within SMa1409 at 774.956 kb on + strand, within SMa1409 at 775.053 kb on + strand, within SMa1409 at 775.924 kb on + strand at 775.924 kb on + strand at 775.925 kb on - strand at 775.945 kb on + strand at 775.946 kb on - strand at 776.029 kb on + strand, within SMa1410 at 776.029 kb on + strand, within SMa1410 at 776.029 kb on + strand, within SMa1410 at 776.090 kb on + strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.091 kb on - strand, within SMa1410 at 776.337 kb on - strand, within SMa1410 at 776.337 kb on - strand, within SMa1410 at 776.337 kb on - strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.562 kb on + strand, within SMa1410 at 776.563 kb on - strand, within SMa1410 at 776.606 kb on + strand, within SMa1410 at 776.606 kb on + strand, within SMa1410 at 776.607 kb on - strand, within SMa1410 at 776.668 kb on + strand at 776.668 kb on + strand at 776.720 kb on - strand at 776.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0005 mg/ml remove 773,210 + SMa1406 0.83 +0.6 773,210 + SMa1406 0.83 -0.4 773,216 + SMa1406 0.83 +0.1 773,583 + SMa1408 0.19 +0.7 773,583 + SMa1408 0.19 -1.0 773,615 - SMa1408 0.23 +1.1 773,615 - SMa1408 0.23 -0.5 773,708 + SMa1408 0.35 +0.7 773,708 + SMa1408 0.35 -0.8 773,708 + SMa1408 0.35 -0.4 773,795 - SMa1408 0.46 +0.0 773,821 + SMa1408 0.50 -0.6 773,821 + SMa1408 0.50 +0.4 773,885 - SMa1408 0.58 -0.1 774,056 - SMa1408 0.80 +2.0 774,056 - SMa1408 0.80 +0.3 774,142 - +0.8 774,142 - +0.4 774,213 + +1.0 774,388 + SMa1409 0.12 -0.5 774,388 + SMa1409 0.12 -1.9 774,388 + SMa1409 0.12 -1.2 774,388 + SMa1409 0.12 +0.1 774,389 - SMa1409 0.12 +0.9 774,389 - SMa1409 0.12 -1.0 774,389 - SMa1409 0.12 -0.7 774,389 - SMa1409 0.12 -1.8 774,566 + SMa1409 0.23 +0.3 774,567 - SMa1409 0.23 +1.2 774,698 - SMa1409 0.31 -1.1 774,860 + SMa1409 0.42 -1.1 774,956 + SMa1409 0.48 -0.3 774,956 + SMa1409 0.48 +1.2 774,956 + SMa1409 0.48 -2.9 775,053 + SMa1409 0.54 +1.1 775,924 + -0.2 775,924 + +0.7 775,925 - +2.5 775,945 + +0.8 775,946 - +1.2 776,029 + SMa1410 0.17 -1.7 776,029 + SMa1410 0.17 -1.2 776,029 + SMa1410 0.17 +0.5 776,090 + SMa1410 0.24 +0.5 776,091 - SMa1410 0.24 -0.1 776,091 - SMa1410 0.24 +1.2 776,091 - SMa1410 0.24 -0.4 776,091 - SMa1410 0.24 +0.9 776,337 - SMa1410 0.54 +0.1 776,337 - SMa1410 0.54 -0.1 776,337 - SMa1410 0.54 +0.9 776,562 + SMa1410 0.81 +0.3 776,562 + SMa1410 0.81 -0.6 776,562 + SMa1410 0.81 +0.3 776,562 + SMa1410 0.81 -0.8 776,562 + SMa1410 0.81 -0.4 776,563 - SMa1410 0.81 +0.4 776,606 + SMa1410 0.86 +0.6 776,606 + SMa1410 0.86 +1.7 776,607 - SMa1410 0.86 -2.1 776,668 + +0.2 776,668 + +0.7 776,720 - +1.3 776,764 + +0.0
Or see this region's nucleotide sequence