Experiment: Cisplatin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20219 and SM_b22002 are separated by 91 nucleotides SM_b22002 and SM_b20221 overlap by 124 nucleotides SM_b20221 and SM_b20222 are separated by 9 nucleotides
SM_b20219: SM_b20219 - response regulator protein, at 227,578 to 228,315
_b20219
SM_b22002: SM_b22002 - epoxide hydrolase, at 228,407 to 228,829
_b22002
SM_b20221: SM_b20221 - epoxide hydrolase, at 228,706 to 229,740
_b20221
SM_b20222: SM_b20222 - hypothetical protein, at 229,750 to 230,172
_b20222
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 227.839 kb on - strand, within SM_b20219 at 228.006 kb on + strand, within SM_b20219 at 228.006 kb on + strand, within SM_b20219 at 228.007 kb on - strand, within SM_b20219 at 228.009 kb on - strand, within SM_b20219 at 228.174 kb on + strand at 228.249 kb on - strand at 228.249 kb on - strand at 228.373 kb on + strand at 228.373 kb on + strand at 228.374 kb on - strand at 228.501 kb on + strand, within SM_b22002 at 228.504 kb on + strand at 228.552 kb on + strand, within SM_b22002 at 228.553 kb on - strand, within SM_b22002 at 228.672 kb on + strand, within SM_b22002 at 228.710 kb on + strand at 228.759 kb on + strand at 228.759 kb on + strand at 228.960 kb on + strand, within SM_b20221 at 228.961 kb on - strand, within SM_b20221 at 229.063 kb on + strand, within SM_b20221 at 229.356 kb on + strand, within SM_b20221 at 229.356 kb on + strand, within SM_b20221 at 229.412 kb on + strand, within SM_b20221 at 229.791 kb on + strand at 229.791 kb on + strand at 229.792 kb on - strand at 229.792 kb on - strand at 229.792 kb on - strand at 229.792 kb on - strand at 229.793 kb on + strand, within SM_b20222 at 229.793 kb on + strand, within SM_b20222 at 229.793 kb on + strand, within SM_b20222 at 229.794 kb on - strand, within SM_b20222 at 229.794 kb on - strand, within SM_b20222 at 229.937 kb on + strand, within SM_b20222 at 229.937 kb on + strand, within SM_b20222 at 229.937 kb on + strand, within SM_b20222 at 229.938 kb on - strand, within SM_b20222 at 229.938 kb on - strand, within SM_b20222 at 229.938 kb on - strand, within SM_b20222 at 230.152 kb on - strand at 230.152 kb on - strand at 230.205 kb on + strand at 230.223 kb on + strand at 230.223 kb on + strand at 230.224 kb on - strand at 230.224 kb on - strand at 230.541 kb on + strand at 230.541 kb on + strand at 230.542 kb on - strand at 230.686 kb on + strand at 230.687 kb on - strand at 230.725 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0005 mg/ml remove 227,839 - SM_b20219 0.35 -0.6 228,006 + SM_b20219 0.58 -3.1 228,006 + SM_b20219 0.58 -1.8 228,007 - SM_b20219 0.58 +1.0 228,009 - SM_b20219 0.58 +1.5 228,174 + +1.2 228,249 - -2.5 228,249 - -1.6 228,373 + +0.2 228,373 + +0.7 228,374 - -1.0 228,501 + SM_b22002 0.22 +0.5 228,504 + +1.2 228,552 + SM_b22002 0.34 -0.5 228,553 - SM_b22002 0.35 -1.8 228,672 + SM_b22002 0.63 +1.0 228,710 + -1.4 228,759 + -0.7 228,759 + -2.5 228,960 + SM_b20221 0.25 -2.7 228,961 - SM_b20221 0.25 +0.5 229,063 + SM_b20221 0.34 +0.2 229,356 + SM_b20221 0.63 -1.1 229,356 + SM_b20221 0.63 -1.8 229,412 + SM_b20221 0.68 -0.8 229,791 + +0.1 229,791 + -0.4 229,792 - +0.2 229,792 - +0.2 229,792 - -0.1 229,792 - +0.2 229,793 + SM_b20222 0.10 -1.0 229,793 + SM_b20222 0.10 -2.6 229,793 + SM_b20222 0.10 -0.8 229,794 - SM_b20222 0.10 -1.1 229,794 - SM_b20222 0.10 +1.2 229,937 + SM_b20222 0.44 +1.0 229,937 + SM_b20222 0.44 +0.2 229,937 + SM_b20222 0.44 -0.4 229,938 - SM_b20222 0.44 +0.5 229,938 - SM_b20222 0.44 -1.0 229,938 - SM_b20222 0.44 +0.2 230,152 - +1.5 230,152 - +0.6 230,205 + -1.6 230,223 + -2.1 230,223 + +0.8 230,224 - +0.1 230,224 - -0.9 230,541 + -1.3 230,541 + -0.7 230,542 - +0.1 230,686 + +0.4 230,687 - +4.2 230,725 + +0.4
Or see this region's nucleotide sequence