Experiment: Cisplatin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b22004 and SM_b20121 overlap by 56 nucleotides SM_b20121 and SM_b20122 are separated by 181 nucleotides SM_b20122 and SM_b20123 are separated by 185 nucleotides SM_b20123 and SM_b20124 are separated by 121 nucleotides
SM_b22004: SM_b22004 - plasmid stabilization system protein, at 132,946 to 133,338
_b22004
SM_b20121: SM_b20121 - hypothetical protein, at 133,283 to 133,558
_b20121
SM_b20122: SM_b20122 - hypothetical protein, at 133,740 to 134,075
_b20122
SM_b20123: SM_b20123 - transcriptional regulator, at 134,261 to 135,157
_b20123
SM_b20124: SM_b20124 - sugar ABC transporter ATP-binding protein, at 135,279 to 136,805
_b20124
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 133.368 kb on - strand, within SM_b20121 at 133.476 kb on + strand, within SM_b20121 at 133.609 kb on - strand at 133.610 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.654 kb on + strand at 133.689 kb on + strand at 133.689 kb on + strand at 133.702 kb on + strand at 133.723 kb on - strand at 133.723 kb on - strand at 133.830 kb on - strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.885 kb on + strand, within SM_b20122 at 133.886 kb on - strand, within SM_b20122 at 133.930 kb on - strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.034 kb on + strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.035 kb on - strand, within SM_b20122 at 134.092 kb on + strand at 134.097 kb on + strand at 134.496 kb on + strand, within SM_b20123 at 134.497 kb on - strand, within SM_b20123 at 134.793 kb on - strand, within SM_b20123 at 134.793 kb on - strand, within SM_b20123 at 134.922 kb on - strand, within SM_b20123 at 135.070 kb on + strand at 135.098 kb on + strand at 135.162 kb on - strand at 135.253 kb on - strand at 135.376 kb on + strand at 135.376 kb on + strand at 135.376 kb on + strand at 135.377 kb on - strand at 135.377 kb on - strand at 135.377 kb on - strand at 135.516 kb on - strand, within SM_b20124 at 135.792 kb on - strand, within SM_b20124 at 135.821 kb on - strand, within SM_b20124 at 135.972 kb on + strand, within SM_b20124 at 135.972 kb on + strand, within SM_b20124 at 135.973 kb on - strand at 135.973 kb on - strand, within SM_b20124 at 136.069 kb on + strand, within SM_b20124 at 136.070 kb on - strand, within SM_b20124 at 136.140 kb on - strand, within SM_b20124
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0005 mg/ml remove 133,368 - SM_b20121 0.31 -0.1 133,476 + SM_b20121 0.70 -0.5 133,609 - +1.5 133,610 + -1.3 133,654 + -0.7 133,654 + +0.1 133,654 + -0.3 133,689 + -1.8 133,689 + -0.7 133,702 + -2.3 133,723 - +0.7 133,723 - +0.3 133,830 - SM_b20122 0.27 +0.9 133,885 + SM_b20122 0.43 +0.4 133,885 + SM_b20122 0.43 -0.5 133,885 + SM_b20122 0.43 -0.3 133,886 - SM_b20122 0.43 -0.1 133,930 - SM_b20122 0.57 -1.3 134,034 + SM_b20122 0.88 -0.7 134,034 + SM_b20122 0.88 +0.2 134,034 + SM_b20122 0.88 -0.2 134,034 + SM_b20122 0.88 +0.3 134,034 + SM_b20122 0.88 +0.3 134,035 - SM_b20122 0.88 -0.5 134,035 - SM_b20122 0.88 +0.5 134,035 - SM_b20122 0.88 -0.8 134,092 + -2.7 134,097 + -2.0 134,496 + SM_b20123 0.26 -1.2 134,497 - SM_b20123 0.26 -1.3 134,793 - SM_b20123 0.59 -1.0 134,793 - SM_b20123 0.59 -0.3 134,922 - SM_b20123 0.74 +0.3 135,070 + -2.8 135,098 + +1.0 135,162 - +0.0 135,253 - -0.1 135,376 + -0.3 135,376 + +1.5 135,376 + -1.0 135,377 - +0.4 135,377 - -0.6 135,377 - -1.3 135,516 - SM_b20124 0.16 -0.7 135,792 - SM_b20124 0.34 -0.7 135,821 - SM_b20124 0.35 +1.3 135,972 + SM_b20124 0.45 +1.4 135,972 + SM_b20124 0.45 +1.3 135,973 - +3.4 135,973 - SM_b20124 0.45 -1.7 136,069 + SM_b20124 0.52 +1.5 136,070 - SM_b20124 0.52 -0.3 136,140 - SM_b20124 0.56 +0.3
Or see this region's nucleotide sequence