Experiment: Cisplatin 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20112 and SM_b20113 are separated by 36 nucleotides SM_b20113 and SM_b20114 are separated by 2 nucleotides SM_b20114 and SM_b20115 are separated by 26 nucleotides
SM_b20112: SM_b20112 - hypothetical protein, at 123,785 to 124,672
_b20112
SM_b20113: SM_b20113 - dehydrogenase, at 124,709 to 125,641
_b20113
SM_b20114: SM_b20114 - hypothetical protein, at 125,644 to 126,369
_b20114
SM_b20115: SM_b20115 - dihydroxy-acid dehydratase, at 126,396 to 128,117
_b20115
Position (kb) 125
126
127 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 124.671 kb on - strand at 124.960 kb on - strand, within SM_b20113 at 125.171 kb on - strand, within SM_b20113 at 125.171 kb on - strand, within SM_b20113 at 125.171 kb on - strand, within SM_b20113 at 125.412 kb on + strand, within SM_b20113 at 125.465 kb on - strand, within SM_b20113 at 125.558 kb on - strand at 125.646 kb on - strand at 125.709 kb on - strand at 126.197 kb on + strand, within SM_b20114 at 126.198 kb on - strand, within SM_b20114 at 126.198 kb on - strand, within SM_b20114 at 126.208 kb on - strand, within SM_b20114 at 126.319 kb on + strand at 126.319 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.359 kb on + strand at 126.360 kb on - strand at 126.360 kb on - strand at 126.360 kb on - strand at 126.503 kb on - strand at 126.515 kb on - strand at 126.558 kb on + strand at 126.688 kb on + strand, within SM_b20115 at 126.773 kb on + strand, within SM_b20115 at 127.009 kb on + strand, within SM_b20115 at 127.010 kb on - strand, within SM_b20115 at 127.010 kb on - strand, within SM_b20115 at 127.162 kb on + strand, within SM_b20115 at 127.162 kb on + strand, within SM_b20115 at 127.163 kb on - strand, within SM_b20115 at 127.180 kb on + strand, within SM_b20115 at 127.181 kb on - strand, within SM_b20115 at 127.181 kb on - strand, within SM_b20115
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0005 mg/ml remove 124,671 - +1.3 124,960 - SM_b20113 0.27 -1.7 125,171 - SM_b20113 0.50 -1.1 125,171 - SM_b20113 0.50 +1.0 125,171 - SM_b20113 0.50 +0.0 125,412 + SM_b20113 0.75 +0.3 125,465 - SM_b20113 0.81 -0.0 125,558 - -3.7 125,646 - -1.6 125,709 - -0.4 126,197 + SM_b20114 0.76 -3.3 126,198 - SM_b20114 0.76 -0.8 126,198 - SM_b20114 0.76 -1.2 126,208 - SM_b20114 0.78 -1.2 126,319 + -0.4 126,319 + -2.2 126,359 + -0.3 126,359 + +0.8 126,359 + +0.9 126,359 + -0.1 126,360 - -0.6 126,360 - -0.1 126,360 - +2.1 126,503 - -0.2 126,515 - +0.6 126,558 + -0.6 126,688 + SM_b20115 0.17 +1.9 126,773 + SM_b20115 0.22 -0.4 127,009 + SM_b20115 0.36 +0.3 127,010 - SM_b20115 0.36 -1.9 127,010 - SM_b20115 0.36 -1.4 127,162 + SM_b20115 0.44 -0.3 127,162 + SM_b20115 0.44 -0.0 127,163 - SM_b20115 0.45 -3.6 127,180 + SM_b20115 0.46 +0.0 127,181 - SM_b20115 0.46 +0.1 127,181 - SM_b20115 0.46 +0.7
Or see this region's nucleotide sequence