Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_749 and Pf1N1B4_750 overlap by 4 nucleotides Pf1N1B4_750 and Pf1N1B4_751 are separated by 178 nucleotides
Pf1N1B4_749 - hypothetical protein, at 852,642 to 852,824
_749
Pf1N1B4_750 - hypothetical protein, at 852,821 to 853,477
_750
Pf1N1B4_751 - hypothetical protein, at 853,656 to 854,096
_751
Position (kb) 853
854
855 Strain fitness (log2 ratio) -2
-1
0
1
2 at 852.794 kb on + strand, within Pf1N1B4_749 at 852.795 kb on - strand, within Pf1N1B4_749 at 852.795 kb on - strand, within Pf1N1B4_749 at 852.850 kb on + strand at 852.850 kb on + strand at 852.850 kb on + strand at 852.850 kb on + strand at 852.851 kb on - strand at 852.936 kb on + strand, within Pf1N1B4_750 at 852.936 kb on + strand, within Pf1N1B4_750 at 852.937 kb on - strand, within Pf1N1B4_750 at 853.188 kb on + strand, within Pf1N1B4_750 at 853.248 kb on + strand at 853.248 kb on + strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.294 kb on + strand, within Pf1N1B4_750 at 853.294 kb on + strand, within Pf1N1B4_750 at 853.311 kb on - strand, within Pf1N1B4_750 at 853.494 kb on + strand at 853.494 kb on + strand at 853.495 kb on - strand at 853.495 kb on - strand at 853.529 kb on + strand at 853.530 kb on - strand at 853.530 kb on - strand at 853.598 kb on - strand at 853.818 kb on + strand, within Pf1N1B4_751 at 853.818 kb on + strand, within Pf1N1B4_751 at 853.819 kb on - strand, within Pf1N1B4_751 at 853.819 kb on - strand, within Pf1N1B4_751 at 854.083 kb on - strand at 854.213 kb on + strand at 854.300 kb on + strand at 854.300 kb on + strand at 854.361 kb on - strand at 854.421 kb on + strand at 854.422 kb on - strand at 854.422 kb on - strand at 854.434 kb on + strand at 854.435 kb on - strand at 854.435 kb on - strand at 854.438 kb on + strand at 854.547 kb on + strand at 854.547 kb on + strand at 854.598 kb on - strand at 854.656 kb on + strand at 854.657 kb on - strand at 854.801 kb on - strand at 854.864 kb on + strand at 854.865 kb on - strand at 854.915 kb on + strand at 854.958 kb on + strand at 854.959 kb on - strand at 854.959 kb on - strand at 854.959 kb on - strand at 854.998 kb on + strand at 854.999 kb on - strand at 854.999 kb on - strand at 855.002 kb on - strand at 855.002 kb on - strand at 855.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 852,794 + Pf1N1B4_749 0.83 +0.8 852,795 - Pf1N1B4_749 0.84 +0.6 852,795 - Pf1N1B4_749 0.84 -1.0 852,850 + -0.7 852,850 + -2.0 852,850 + -1.7 852,850 + -1.5 852,851 - +0.5 852,936 + Pf1N1B4_750 0.18 +2.4 852,936 + Pf1N1B4_750 0.18 +1.0 852,937 - Pf1N1B4_750 0.18 +2.0 853,188 + Pf1N1B4_750 0.56 -0.3 853,248 + +1.0 853,248 + Pf1N1B4_750 0.65 -1.3 853,249 - Pf1N1B4_750 0.65 +1.8 853,249 - Pf1N1B4_750 0.65 -2.0 853,249 - Pf1N1B4_750 0.65 -1.5 853,249 - Pf1N1B4_750 0.65 +2.4 853,249 - Pf1N1B4_750 0.65 -0.3 853,249 - Pf1N1B4_750 0.65 +0.9 853,249 - Pf1N1B4_750 0.65 +1.3 853,249 - Pf1N1B4_750 0.65 +1.0 853,294 + Pf1N1B4_750 0.72 +1.5 853,294 + Pf1N1B4_750 0.72 -0.1 853,311 - Pf1N1B4_750 0.75 +0.4 853,494 + -1.0 853,494 + -0.4 853,495 - +0.0 853,495 - -2.4 853,529 + +0.5 853,530 - +1.0 853,530 - +0.3 853,598 - -1.8 853,818 + Pf1N1B4_751 0.37 -0.8 853,818 + Pf1N1B4_751 0.37 +1.2 853,819 - Pf1N1B4_751 0.37 +0.9 853,819 - Pf1N1B4_751 0.37 +1.0 854,083 - +1.3 854,213 + +1.5 854,300 + -0.3 854,300 + +1.0 854,361 - -0.8 854,421 + +1.6 854,422 - -0.3 854,422 - -0.5 854,434 + +0.0 854,435 - +2.4 854,435 - -2.0 854,438 + +0.0 854,547 + -1.0 854,547 + +1.0 854,598 - +0.0 854,656 + +0.6 854,657 - +0.7 854,801 - +1.8 854,864 + +0.9 854,865 - -1.3 854,915 + -1.3 854,958 + +0.0 854,959 - +0.3 854,959 - -0.4 854,959 - -0.5 854,998 + -0.0 854,999 - +1.5 854,999 - +0.6 855,002 - -1.3 855,002 - +0.8 855,002 - +2.6
Or see this region's nucleotide sequence