Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_626 and Pf1N1B4_627 overlap by 33 nucleotides Pf1N1B4_627 and Pf1N1B4_5174 are separated by 170 nucleotides Pf1N1B4_5174 and Pf1N1B4_628 are separated by 63 nucleotides
Pf1N1B4_626 - Quinolinate synthetase (EC 2.5.1.72), at 724,431 to 725,489
_626
Pf1N1B4_627 - hypothetical protein, at 725,457 to 725,573
_627
Pf1N1B4_5174 - tRNA-Lys-TTT, at 725,744 to 725,816
_5174
Pf1N1B4_628 - Queuosine Biosynthesis QueC ATPase, at 725,880 to 726,560
_628
Position (kb) 725
726 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 724.610 kb on + strand, within Pf1N1B4_626 at 724.935 kb on - strand, within Pf1N1B4_626 at 724.936 kb on + strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 725.072 kb on - strand at 725.072 kb on - strand, within Pf1N1B4_626 at 725.072 kb on - strand, within Pf1N1B4_626 at 725.084 kb on + strand, within Pf1N1B4_626 at 725.084 kb on + strand, within Pf1N1B4_626 at 725.085 kb on - strand, within Pf1N1B4_626 at 725.085 kb on - strand, within Pf1N1B4_626 at 725.341 kb on + strand, within Pf1N1B4_626 at 725.341 kb on + strand, within Pf1N1B4_626 at 725.342 kb on - strand, within Pf1N1B4_626 at 725.397 kb on - strand at 725.404 kb on + strand at 725.405 kb on - strand at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand, within Pf1N1B4_627 at 725.532 kb on - strand at 725.576 kb on - strand at 725.576 kb on - strand at 725.576 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.715 kb on - strand at 725.887 kb on + strand at 725.887 kb on + strand at 725.955 kb on - strand, within Pf1N1B4_628 at 726.096 kb on + strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.097 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.176 kb on - strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.274 kb on + strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.275 kb on - strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.320 kb on + strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.321 kb on - strand, within Pf1N1B4_628 at 726.446 kb on - strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.463 kb on + strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628 at 726.464 kb on - strand, within Pf1N1B4_628
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 724,610 + Pf1N1B4_626 0.17 -1.5 724,935 - Pf1N1B4_626 0.48 -0.3 724,936 + Pf1N1B4_626 0.48 +4.2 724,937 - Pf1N1B4_626 0.48 -1.6 724,937 - Pf1N1B4_626 0.48 +1.1 724,937 - Pf1N1B4_626 0.48 -0.4 724,937 - Pf1N1B4_626 0.48 +2.0 724,937 - Pf1N1B4_626 0.48 -1.0 724,937 - Pf1N1B4_626 0.48 -0.9 724,937 - Pf1N1B4_626 0.48 -1.3 724,937 - Pf1N1B4_626 0.48 -0.2 724,937 - Pf1N1B4_626 0.48 +1.6 724,937 - Pf1N1B4_626 0.48 -0.4 724,937 - Pf1N1B4_626 0.48 -2.3 724,937 - Pf1N1B4_626 0.48 +1.0 725,072 - -1.0 725,072 - Pf1N1B4_626 0.61 +1.0 725,072 - Pf1N1B4_626 0.61 -0.4 725,084 + Pf1N1B4_626 0.62 -0.5 725,084 + Pf1N1B4_626 0.62 +0.7 725,085 - Pf1N1B4_626 0.62 -0.0 725,085 - Pf1N1B4_626 0.62 +0.1 725,341 + Pf1N1B4_626 0.86 +1.8 725,341 + Pf1N1B4_626 0.86 -1.5 725,342 - Pf1N1B4_626 0.86 -0.2 725,397 - -1.3 725,404 + -1.6 725,405 - -0.7 725,532 - Pf1N1B4_627 0.64 +1.0 725,532 - Pf1N1B4_627 0.64 +0.5 725,532 - Pf1N1B4_627 0.64 -1.1 725,532 - Pf1N1B4_627 0.64 -1.0 725,532 - Pf1N1B4_627 0.64 -2.5 725,532 - Pf1N1B4_627 0.64 -1.3 725,532 - Pf1N1B4_627 0.64 -2.7 725,532 - -0.6 725,576 - -0.4 725,576 - +1.0 725,576 - -0.6 725,715 - +1.0 725,715 - -0.6 725,715 - -2.8 725,715 - +0.6 725,887 + -1.0 725,887 + +0.6 725,955 - Pf1N1B4_628 0.11 +2.0 726,096 + Pf1N1B4_628 0.32 +1.4 726,097 - Pf1N1B4_628 0.32 -0.6 726,097 - Pf1N1B4_628 0.32 +0.3 726,176 - Pf1N1B4_628 0.43 -0.0 726,176 - Pf1N1B4_628 0.43 +1.7 726,274 + Pf1N1B4_628 0.58 -0.0 726,274 + Pf1N1B4_628 0.58 +0.5 726,274 + Pf1N1B4_628 0.58 +0.2 726,274 + Pf1N1B4_628 0.58 -0.9 726,275 - Pf1N1B4_628 0.58 +1.0 726,275 - Pf1N1B4_628 0.58 +1.8 726,275 - Pf1N1B4_628 0.58 +1.0 726,320 + Pf1N1B4_628 0.65 +0.7 726,320 + Pf1N1B4_628 0.65 +0.5 726,321 - Pf1N1B4_628 0.65 +0.3 726,321 - Pf1N1B4_628 0.65 -0.6 726,321 - Pf1N1B4_628 0.65 +0.3 726,321 - Pf1N1B4_628 0.65 -1.2 726,321 - Pf1N1B4_628 0.65 +0.3 726,321 - Pf1N1B4_628 0.65 -0.0 726,446 - Pf1N1B4_628 0.83 +2.8 726,463 + Pf1N1B4_628 0.86 +0.5 726,463 + Pf1N1B4_628 0.86 -1.8 726,463 + Pf1N1B4_628 0.86 -1.6 726,464 - Pf1N1B4_628 0.86 +0.7 726,464 - Pf1N1B4_628 0.86 +0.5
Or see this region's nucleotide sequence