Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5992 and Pf1N1B4_5993 overlap by 4 nucleotides Pf1N1B4_5993 and Pf1N1B4_5994 are separated by 14 nucleotides Pf1N1B4_5994 and Pf1N1B4_5995 overlap by 4 nucleotides
Pf1N1B4_5992 - Inner membrane protein, at 443,714 to 444,724
_5992
Pf1N1B4_5993 - Ferredoxin, 2Fe-2S, at 444,721 to 445,029
_5993
Pf1N1B4_5994 - 2-hydroxychromene-2-carboxylate isomerase/DsbA-like thioredoxin domain, at 445,044 to 445,706
_5994
Pf1N1B4_5995 - Transporter, MFS superfamily, at 445,703 to 447,034
_5995
Position (kb) 445
446 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 444.515 kb on - strand, within Pf1N1B4_5992 at 444.757 kb on + strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.822 kb on - strand, within Pf1N1B4_5993 at 445.357 kb on - strand, within Pf1N1B4_5994 at 445.401 kb on - strand, within Pf1N1B4_5994 at 445.618 kb on - strand, within Pf1N1B4_5994 at 445.688 kb on + strand at 445.689 kb on - strand at 445.692 kb on + strand at 445.693 kb on - strand at 445.693 kb on - strand at 445.759 kb on + strand at 445.797 kb on + strand at 445.798 kb on - strand at 446.247 kb on + strand, within Pf1N1B4_5995 at 446.263 kb on - strand, within Pf1N1B4_5995 at 446.323 kb on - strand, within Pf1N1B4_5995 at 446.357 kb on - strand, within Pf1N1B4_5995 at 446.544 kb on - strand, within Pf1N1B4_5995 at 446.698 kb on - strand, within Pf1N1B4_5995
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 444,515 - Pf1N1B4_5992 0.79 -0.0 444,757 + Pf1N1B4_5993 0.12 -1.0 444,800 - Pf1N1B4_5993 0.26 +0.5 444,800 - Pf1N1B4_5993 0.26 +3.0 444,822 - Pf1N1B4_5993 0.33 -1.3 445,357 - Pf1N1B4_5994 0.47 -0.8 445,401 - Pf1N1B4_5994 0.54 +0.1 445,618 - Pf1N1B4_5994 0.87 +1.6 445,688 + +0.1 445,689 - -1.8 445,692 + -0.5 445,693 - -1.5 445,693 - -0.3 445,759 + +1.4 445,797 + +0.2 445,798 - +1.0 446,247 + Pf1N1B4_5995 0.41 -2.5 446,263 - Pf1N1B4_5995 0.42 +0.2 446,323 - Pf1N1B4_5995 0.47 -1.6 446,357 - Pf1N1B4_5995 0.49 -0.0 446,544 - Pf1N1B4_5995 0.63 -0.8 446,698 - Pf1N1B4_5995 0.75 -1.8
Or see this region's nucleotide sequence