Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5875 and Pf1N1B4_5876 overlap by 4 nucleotides Pf1N1B4_5876 and Pf1N1B4_5877 are separated by 332 nucleotides Pf1N1B4_5877 and Pf1N1B4_5878 are separated by 86 nucleotides Pf1N1B4_5878 and Pf1N1B4_5879 are separated by 10 nucleotides Pf1N1B4_5879 and Pf1N1B4_5880 are separated by 163 nucleotides
Pf1N1B4_5875 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 317,897 to 318,244
_5875
Pf1N1B4_5876 - hypothetical protein, at 318,241 to 318,507
_5876
Pf1N1B4_5877 - FIG00967533: hypothetical protein, at 318,840 to 319,424
_5877
Pf1N1B4_5878 - hypothetical protein, at 319,511 to 319,684
_5878
Pf1N1B4_5879 - hypothetical protein, at 319,695 to 319,937
_5879
Pf1N1B4_5880 - AttH component of AttEFGH ABC transport system, at 320,101 to 321,174
_5880
Position (kb) 318
319
320 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 318.048 kb on + strand, within Pf1N1B4_5875 at 318.250 kb on + strand at 318.550 kb on + strand at 318.551 kb on - strand at 318.602 kb on - strand at 318.694 kb on - strand at 318.748 kb on - strand at 318.795 kb on + strand at 318.796 kb on - strand at 318.798 kb on - strand at 318.824 kb on + strand at 318.825 kb on - strand at 318.829 kb on - strand at 318.835 kb on - strand at 318.835 kb on - strand at 318.835 kb on - strand at 318.880 kb on + strand at 318.880 kb on + strand at 318.880 kb on + strand at 318.880 kb on + strand at 318.881 kb on - strand at 319.036 kb on - strand, within Pf1N1B4_5877 at 319.036 kb on - strand, within Pf1N1B4_5877 at 319.036 kb on - strand, within Pf1N1B4_5877 at 319.097 kb on + strand at 319.144 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.231 kb on - strand, within Pf1N1B4_5877 at 319.424 kb on + strand at 319.425 kb on - strand at 319.431 kb on + strand at 319.467 kb on + strand at 319.501 kb on + strand at 319.502 kb on - strand at 319.502 kb on - strand at 319.502 kb on - strand at 319.833 kb on + strand, within Pf1N1B4_5879 at 319.957 kb on + strand at 319.958 kb on - strand at 320.010 kb on - strand at 320.059 kb on - strand at 320.079 kb on + strand at 320.079 kb on + strand at 320.080 kb on - strand at 320.102 kb on + strand at 320.103 kb on - strand at 320.103 kb on - strand at 320.124 kb on - strand at 320.372 kb on + strand, within Pf1N1B4_5880
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 318,048 + Pf1N1B4_5875 0.43 -1.8 318,250 + -0.9 318,550 + -0.2 318,551 - -1.5 318,602 - -0.6 318,694 - +0.5 318,748 - -0.8 318,795 + -0.5 318,796 - -1.8 318,798 - -0.2 318,824 + -1.3 318,825 - -1.5 318,829 - +0.2 318,835 - -0.5 318,835 - -0.2 318,835 - +0.2 318,880 + +0.5 318,880 + -0.3 318,880 + +0.7 318,880 + +1.3 318,881 - -1.0 319,036 - Pf1N1B4_5877 0.34 +0.1 319,036 - Pf1N1B4_5877 0.34 -0.6 319,036 - Pf1N1B4_5877 0.34 -2.3 319,097 + +0.5 319,144 + Pf1N1B4_5877 0.52 -0.5 319,230 + Pf1N1B4_5877 0.67 -1.9 319,230 + Pf1N1B4_5877 0.67 -0.2 319,230 + Pf1N1B4_5877 0.67 -1.8 319,231 - Pf1N1B4_5877 0.67 -1.8 319,424 + +1.5 319,425 - -0.8 319,431 + +0.1 319,467 + +0.8 319,501 + +0.8 319,502 - +2.0 319,502 - -0.5 319,502 - +1.0 319,833 + Pf1N1B4_5879 0.57 -1.0 319,957 + +0.3 319,958 - -0.5 320,010 - -1.4 320,059 - -0.5 320,079 + -0.7 320,079 + +3.2 320,080 - +3.7 320,102 + +0.5 320,103 - +1.3 320,103 - +0.1 320,124 - +0.3 320,372 + Pf1N1B4_5880 0.25 -0.5
Or see this region's nucleotide sequence