Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5550

Experiment: Sisomicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5549 and Pf1N1B4_5550 are separated by 322 nucleotidesPf1N1B4_5550 and Pf1N1B4_5551 are separated by 148 nucleotides Pf1N1B4_5549 - hypothetical protein, at 320,933 to 321,061 _5549 Pf1N1B4_5550 - Group 2 RNA polymerase sigma factor @ Cyanobacteria-specific RpoD-like sigma factor, type-7, at 321,384 to 322,937 _5550 Pf1N1B4_5551 - hypothetical protein, at 323,086 to 324,072 _5551 Position (kb) 321 322 323Strain fitness (log2 ratio) -2 -1 0 1 2 3at 320.409 kb on - strandat 320.558 kb on + strandat 320.558 kb on + strandat 320.559 kb on - strandat 320.622 kb on + strandat 320.622 kb on + strandat 320.622 kb on + strandat 320.623 kb on - strandat 320.623 kb on - strandat 320.623 kb on - strandat 320.681 kb on + strandat 320.682 kb on - strandat 320.682 kb on - strandat 320.682 kb on - strandat 320.685 kb on + strandat 320.685 kb on + strandat 320.685 kb on + strandat 320.685 kb on + strandat 320.685 kb on + strandat 320.685 kb on + strandat 320.686 kb on - strandat 320.686 kb on - strandat 320.686 kb on - strandat 320.686 kb on - strandat 320.686 kb on - strandat 320.686 kb on - strandat 320.735 kb on - strandat 320.787 kb on + strandat 320.788 kb on - strandat 320.788 kb on - strandat 320.848 kb on + strandat 320.849 kb on - strandat 320.935 kb on - strandat 320.995 kb on + strand, within Pf1N1B4_5549at 320.995 kb on + strand, within Pf1N1B4_5549at 320.995 kb on + strand, within Pf1N1B4_5549at 320.996 kb on - strand, within Pf1N1B4_5549at 320.996 kb on - strand, within Pf1N1B4_5549at 320.996 kb on - strand, within Pf1N1B4_5549at 320.996 kb on - strand, within Pf1N1B4_5549at 321.053 kb on - strandat 321.053 kb on - strandat 321.208 kb on + strandat 321.223 kb on + strandat 321.427 kb on - strandat 321.427 kb on - strandat 321.435 kb on + strandat 321.595 kb on + strand, within Pf1N1B4_5550at 321.595 kb on + strand, within Pf1N1B4_5550at 321.596 kb on - strand, within Pf1N1B4_5550at 321.596 kb on - strand, within Pf1N1B4_5550at 321.596 kb on - strand, within Pf1N1B4_5550at 321.596 kb on - strand, within Pf1N1B4_5550at 321.596 kb on - strand, within Pf1N1B4_5550at 321.628 kb on - strand, within Pf1N1B4_5550at 321.628 kb on - strand, within Pf1N1B4_5550at 321.724 kb on + strand, within Pf1N1B4_5550at 321.724 kb on + strandat 321.734 kb on - strand, within Pf1N1B4_5550at 321.926 kb on - strand, within Pf1N1B4_5550at 321.926 kb on - strand, within Pf1N1B4_5550at 321.926 kb on - strand, within Pf1N1B4_5550at 321.958 kb on + strand, within Pf1N1B4_5550at 321.959 kb on - strand, within Pf1N1B4_5550at 321.959 kb on - strand, within Pf1N1B4_5550at 321.959 kb on - strand, within Pf1N1B4_5550at 322.013 kb on + strand, within Pf1N1B4_5550at 322.014 kb on - strand, within Pf1N1B4_5550at 322.014 kb on - strand, within Pf1N1B4_5550at 322.014 kb on - strand, within Pf1N1B4_5550at 322.031 kb on - strand, within Pf1N1B4_5550at 322.201 kb on - strand, within Pf1N1B4_5550at 322.201 kb on - strand, within Pf1N1B4_5550at 322.230 kb on - strand, within Pf1N1B4_5550at 322.230 kb on - strand, within Pf1N1B4_5550at 322.280 kb on - strand, within Pf1N1B4_5550at 322.280 kb on - strand, within Pf1N1B4_5550at 322.320 kb on + strand, within Pf1N1B4_5550at 322.320 kb on + strand, within Pf1N1B4_5550at 322.321 kb on - strand, within Pf1N1B4_5550at 322.437 kb on - strand, within Pf1N1B4_5550at 322.490 kb on + strand, within Pf1N1B4_5550at 322.636 kb on + strand, within Pf1N1B4_5550at 322.734 kb on - strand, within Pf1N1B4_5550at 322.801 kb on - strandat 322.839 kb on + strandat 322.839 kb on + strandat 322.878 kb on + strandat 322.878 kb on + strandat 322.879 kb on - strandat 322.879 kb on - strandat 322.903 kb on - strandat 322.937 kb on + strandat 322.937 kb on + strandat 322.937 kb on + strandat 322.937 kb on + strandat 322.937 kb on + strandat 322.937 kb on + strandat 322.937 kb on + strandat 322.938 kb on - strandat 322.938 kb on - strandat 322.938 kb on - strandat 322.938 kb on - strandat 322.938 kb on - strandat 323.049 kb on + strandat 323.049 kb on + strandat 323.093 kb on - strandat 323.093 kb on - strandat 323.181 kb on + strandat 323.208 kb on + strand, within Pf1N1B4_5551at 323.224 kb on + strand, within Pf1N1B4_5551at 323.224 kb on + strand, within Pf1N1B4_5551at 323.224 kb on + strand, within Pf1N1B4_5551at 323.224 kb on + strand, within Pf1N1B4_5551at 323.225 kb on - strand, within Pf1N1B4_5551at 323.225 kb on - strand, within Pf1N1B4_5551at 323.243 kb on + strand, within Pf1N1B4_5551at 323.243 kb on + strand, within Pf1N1B4_5551at 323.464 kb on - strand, within Pf1N1B4_5551at 323.464 kb on - strand, within Pf1N1B4_5551at 323.498 kb on + strand, within Pf1N1B4_5551at 323.499 kb on - strand, within Pf1N1B4_5551at 323.564 kb on - strand, within Pf1N1B4_5551at 323.576 kb on + strand, within Pf1N1B4_5551at 323.576 kb on + strand, within Pf1N1B4_5551at 323.576 kb on + strandat 323.577 kb on - strand, within Pf1N1B4_5551at 323.577 kb on - strand, within Pf1N1B4_5551at 323.577 kb on - strand, within Pf1N1B4_5551at 323.791 kb on + strand, within Pf1N1B4_5551at 323.791 kb on + strand, within Pf1N1B4_5551at 323.791 kb on + strand, within Pf1N1B4_5551at 323.792 kb on - strand, within Pf1N1B4_5551at 323.935 kb on + strand, within Pf1N1B4_5551

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml
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320,409 - +0.4
320,558 + +1.9
320,558 + +1.1
320,559 - -0.6
320,622 + +0.4
320,622 + +1.1
320,622 + +1.9
320,623 - +1.2
320,623 - +0.4
320,623 - +0.2
320,681 + -1.4
320,682 - +1.2
320,682 - +2.2
320,682 - +0.1
320,685 + -0.1
320,685 + +0.5
320,685 + -0.8
320,685 + +2.5
320,685 + +1.0
320,685 + -1.0
320,686 - +0.8
320,686 - +1.2
320,686 - +1.2
320,686 - +0.7
320,686 - +0.2
320,686 - +0.6
320,735 - +1.8
320,787 + +1.7
320,788 - +0.2
320,788 - +2.4
320,848 + +1.6
320,849 - -1.0
320,935 - +0.8
320,995 + Pf1N1B4_5549 0.48 +0.2
320,995 + Pf1N1B4_5549 0.48 -1.8
320,995 + Pf1N1B4_5549 0.48 +0.4
320,996 - Pf1N1B4_5549 0.49 +0.2
320,996 - Pf1N1B4_5549 0.49 +0.2
320,996 - Pf1N1B4_5549 0.49 -1.4
320,996 - Pf1N1B4_5549 0.49 +1.4
321,053 - -0.4
321,053 - -0.7
321,208 + -0.1
321,223 + +1.8
321,427 - -1.1
321,427 - +1.5
321,435 + +1.0
321,595 + Pf1N1B4_5550 0.14 -0.4
321,595 + Pf1N1B4_5550 0.14 -0.6
321,596 - Pf1N1B4_5550 0.14 +1.2
321,596 - Pf1N1B4_5550 0.14 +1.0
321,596 - Pf1N1B4_5550 0.14 -1.0
321,596 - Pf1N1B4_5550 0.14 +0.5
321,596 - Pf1N1B4_5550 0.14 +0.2
321,628 - Pf1N1B4_5550 0.16 +0.4
321,628 - Pf1N1B4_5550 0.16 -1.0
321,724 + Pf1N1B4_5550 0.22 +1.6
321,724 + -0.4
321,734 - Pf1N1B4_5550 0.23 +0.8
321,926 - Pf1N1B4_5550 0.35 +0.2
321,926 - Pf1N1B4_5550 0.35 -1.4
321,926 - Pf1N1B4_5550 0.35 -0.2
321,958 + Pf1N1B4_5550 0.37 +1.9
321,959 - Pf1N1B4_5550 0.37 +0.2
321,959 - Pf1N1B4_5550 0.37 -0.6
321,959 - Pf1N1B4_5550 0.37 +0.2
322,013 + Pf1N1B4_5550 0.40 +1.9
322,014 - Pf1N1B4_5550 0.41 +0.4
322,014 - Pf1N1B4_5550 0.41 -0.3
322,014 - Pf1N1B4_5550 0.41 -0.1
322,031 - Pf1N1B4_5550 0.42 -0.4
322,201 - Pf1N1B4_5550 0.53 +2.5
322,201 - Pf1N1B4_5550 0.53 -2.0
322,230 - Pf1N1B4_5550 0.54 +0.8
322,230 - Pf1N1B4_5550 0.54 -0.5
322,280 - Pf1N1B4_5550 0.58 +1.6
322,280 - Pf1N1B4_5550 0.58 -0.9
322,320 + Pf1N1B4_5550 0.60 +1.0
322,320 + Pf1N1B4_5550 0.60 +0.2
322,321 - Pf1N1B4_5550 0.60 +1.2
322,437 - Pf1N1B4_5550 0.68 +2.8
322,490 + Pf1N1B4_5550 0.71 +1.8
322,636 + Pf1N1B4_5550 0.81 +1.2
322,734 - Pf1N1B4_5550 0.87 -0.1
322,801 - +1.4
322,839 + +0.6
322,839 + +0.5
322,878 + -1.6
322,878 + -0.4
322,879 - -0.4
322,879 - -1.1
322,903 - -0.1
322,937 + -1.0
322,937 + -1.1
322,937 + +1.7
322,937 + +0.2
322,937 + +0.4
322,937 + +1.2
322,937 + +0.3
322,938 - +0.6
322,938 - -1.1
322,938 - -1.3
322,938 - +0.5
322,938 - +2.6
323,049 + +0.6
323,049 + +0.7
323,093 - -2.4
323,093 - +0.9
323,181 + -0.4
323,208 + Pf1N1B4_5551 0.12 +0.2
323,224 + Pf1N1B4_5551 0.14 -1.1
323,224 + Pf1N1B4_5551 0.14 -2.3
323,224 + Pf1N1B4_5551 0.14 -0.5
323,224 + Pf1N1B4_5551 0.14 +0.6
323,225 - Pf1N1B4_5551 0.14 -0.4
323,225 - Pf1N1B4_5551 0.14 -0.1
323,243 + Pf1N1B4_5551 0.16 -1.1
323,243 + Pf1N1B4_5551 0.16 +0.2
323,464 - Pf1N1B4_5551 0.38 +3.0
323,464 - Pf1N1B4_5551 0.38 +1.8
323,498 + Pf1N1B4_5551 0.42 -0.1
323,499 - Pf1N1B4_5551 0.42 -1.8
323,564 - Pf1N1B4_5551 0.48 +0.6
323,576 + Pf1N1B4_5551 0.50 -0.4
323,576 + Pf1N1B4_5551 0.50 -0.2
323,576 + -0.1
323,577 - Pf1N1B4_5551 0.50 +0.4
323,577 - Pf1N1B4_5551 0.50 -1.1
323,577 - Pf1N1B4_5551 0.50 +0.8
323,791 + Pf1N1B4_5551 0.71 -0.8
323,791 + Pf1N1B4_5551 0.71 +0.4
323,791 + Pf1N1B4_5551 0.71 -0.3
323,792 - Pf1N1B4_5551 0.72 -0.8
323,935 + Pf1N1B4_5551 0.86 +0.6

Or see this region's nucleotide sequence