Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5548 and Pf1N1B4_5549 are separated by 989 nucleotides Pf1N1B4_5549 and Pf1N1B4_5550 are separated by 322 nucleotides
Pf1N1B4_5548 - hypothetical protein, at 318,930 to 319,943
_5548
Pf1N1B4_5549 - hypothetical protein, at 320,933 to 321,061
_5549
Pf1N1B4_5550 - Group 2 RNA polymerase sigma factor @ Cyanobacteria-specific RpoD-like sigma factor, type-7, at 321,384 to 322,937
_5550
Position (kb) 320
321
322 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 319.947 kb on + strand at 319.948 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.954 kb on - strand at 319.966 kb on + strand at 319.971 kb on + strand at 319.975 kb on - strand at 319.975 kb on - strand at 320.021 kb on + strand at 320.022 kb on - strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.135 kb on + strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.136 kb on - strand at 320.174 kb on + strand at 320.174 kb on + strand at 320.175 kb on - strand at 320.175 kb on - strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.178 kb on + strand at 320.179 kb on - strand at 320.179 kb on - strand at 320.200 kb on - strand at 320.255 kb on + strand at 320.256 kb on - strand at 320.277 kb on - strand at 320.282 kb on - strand at 320.339 kb on + strand at 320.339 kb on + strand at 320.340 kb on - strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.365 kb on + strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.367 kb on + strand at 320.367 kb on + strand at 320.368 kb on - strand at 320.409 kb on - strand at 320.558 kb on + strand at 320.558 kb on + strand at 320.559 kb on - strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.622 kb on + strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.623 kb on - strand at 320.681 kb on + strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.682 kb on - strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.685 kb on + strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.686 kb on - strand at 320.735 kb on - strand at 320.787 kb on + strand at 320.788 kb on - strand at 320.788 kb on - strand at 320.848 kb on + strand at 320.849 kb on - strand at 320.935 kb on - strand at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.995 kb on + strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 320.996 kb on - strand, within Pf1N1B4_5549 at 321.053 kb on - strand at 321.053 kb on - strand at 321.208 kb on + strand at 321.223 kb on + strand at 321.427 kb on - strand at 321.427 kb on - strand at 321.435 kb on + strand at 321.595 kb on + strand, within Pf1N1B4_5550 at 321.595 kb on + strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.596 kb on - strand, within Pf1N1B4_5550 at 321.628 kb on - strand, within Pf1N1B4_5550 at 321.628 kb on - strand, within Pf1N1B4_5550 at 321.724 kb on + strand, within Pf1N1B4_5550 at 321.724 kb on + strand at 321.734 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.926 kb on - strand, within Pf1N1B4_5550 at 321.958 kb on + strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 321.959 kb on - strand, within Pf1N1B4_5550 at 322.013 kb on + strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.014 kb on - strand, within Pf1N1B4_5550 at 322.031 kb on - strand, within Pf1N1B4_5550
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 319,947 + -0.7 319,948 - +1.0 319,954 - +1.2 319,954 - -0.6 319,954 - -0.1 319,954 - +0.8 319,966 + +0.3 319,971 + -1.4 319,975 - -0.4 319,975 - -0.6 320,021 + +2.2 320,022 - +3.3 320,135 + +1.5 320,135 + -0.0 320,135 + -0.4 320,135 + -1.6 320,135 + -0.4 320,135 + -1.4 320,135 + -1.0 320,136 - -0.5 320,136 - -0.4 320,136 - +0.8 320,136 - -0.0 320,136 - +0.4 320,174 + +1.8 320,174 + -1.1 320,175 - +0.3 320,175 - +0.6 320,178 + +0.2 320,178 + +0.9 320,178 + +0.6 320,178 + +1.2 320,178 + +0.9 320,179 - -0.1 320,179 - +0.9 320,200 - +0.6 320,255 + -0.5 320,256 - -0.2 320,277 - -0.6 320,282 - +1.0 320,339 + +0.2 320,339 + -0.7 320,340 - +0.9 320,365 + +0.3 320,365 + +1.2 320,365 + +0.1 320,366 - -0.3 320,366 - -0.8 320,366 - +0.6 320,366 - +0.3 320,366 - -1.4 320,367 + -1.4 320,367 + -1.1 320,368 - -0.1 320,409 - +0.4 320,558 + +1.9 320,558 + +1.1 320,559 - -0.6 320,622 + +0.4 320,622 + +1.1 320,622 + +1.9 320,623 - +1.2 320,623 - +0.4 320,623 - +0.2 320,681 + -1.4 320,682 - +1.2 320,682 - +2.2 320,682 - +0.1 320,685 + -0.1 320,685 + +0.5 320,685 + -0.8 320,685 + +2.5 320,685 + +1.0 320,685 + -1.0 320,686 - +0.8 320,686 - +1.2 320,686 - +1.2 320,686 - +0.7 320,686 - +0.2 320,686 - +0.6 320,735 - +1.8 320,787 + +1.7 320,788 - +0.2 320,788 - +2.4 320,848 + +1.6 320,849 - -1.0 320,935 - +0.8 320,995 + Pf1N1B4_5549 0.48 +0.2 320,995 + Pf1N1B4_5549 0.48 -1.8 320,995 + Pf1N1B4_5549 0.48 +0.4 320,996 - Pf1N1B4_5549 0.49 +0.2 320,996 - Pf1N1B4_5549 0.49 +0.2 320,996 - Pf1N1B4_5549 0.49 -1.4 320,996 - Pf1N1B4_5549 0.49 +1.4 321,053 - -0.4 321,053 - -0.7 321,208 + -0.1 321,223 + +1.8 321,427 - -1.1 321,427 - +1.5 321,435 + +1.0 321,595 + Pf1N1B4_5550 0.14 -0.4 321,595 + Pf1N1B4_5550 0.14 -0.6 321,596 - Pf1N1B4_5550 0.14 +1.2 321,596 - Pf1N1B4_5550 0.14 +1.0 321,596 - Pf1N1B4_5550 0.14 -1.0 321,596 - Pf1N1B4_5550 0.14 +0.5 321,596 - Pf1N1B4_5550 0.14 +0.2 321,628 - Pf1N1B4_5550 0.16 +0.4 321,628 - Pf1N1B4_5550 0.16 -1.0 321,724 + Pf1N1B4_5550 0.22 +1.6 321,724 + -0.4 321,734 - Pf1N1B4_5550 0.23 +0.8 321,926 - Pf1N1B4_5550 0.35 +0.2 321,926 - Pf1N1B4_5550 0.35 -1.4 321,926 - Pf1N1B4_5550 0.35 -0.2 321,958 + Pf1N1B4_5550 0.37 +1.9 321,959 - Pf1N1B4_5550 0.37 +0.2 321,959 - Pf1N1B4_5550 0.37 -0.6 321,959 - Pf1N1B4_5550 0.37 +0.2 322,013 + Pf1N1B4_5550 0.40 +1.9 322,014 - Pf1N1B4_5550 0.41 +0.4 322,014 - Pf1N1B4_5550 0.41 -0.3 322,014 - Pf1N1B4_5550 0.41 -0.1 322,031 - Pf1N1B4_5550 0.42 -0.4
Or see this region's nucleotide sequence