Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5547

Experiment: Sisomicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5545 and Pf1N1B4_5546 overlap by 1 nucleotidesPf1N1B4_5546 and Pf1N1B4_5547 are separated by 37 nucleotidesPf1N1B4_5547 and Pf1N1B4_5548 are separated by 150 nucleotides Pf1N1B4_5545 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 311,793 to 317,519 _5545 Pf1N1B4_5546 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 317,519 to 318,460 _5546 Pf1N1B4_5547 - hypothetical protein, at 318,498 to 318,779 _5547 Pf1N1B4_5548 - hypothetical protein, at 318,930 to 319,943 _5548 Position (kb) 318 319Strain fitness (log2 ratio) -2 -1 0 1 2 3at 317.518 kb on - strandat 317.532 kb on + strandat 317.533 kb on - strandat 317.533 kb on - strandat 317.533 kb on - strandat 317.823 kb on + strand, within Pf1N1B4_5546at 317.878 kb on + strand, within Pf1N1B4_5546at 317.892 kb on + strand, within Pf1N1B4_5546at 317.892 kb on + strand, within Pf1N1B4_5546at 317.892 kb on + strand, within Pf1N1B4_5546at 317.893 kb on - strand, within Pf1N1B4_5546at 317.940 kb on + strand, within Pf1N1B4_5546at 317.948 kb on + strand, within Pf1N1B4_5546at 317.948 kb on + strand, within Pf1N1B4_5546at 317.985 kb on + strand, within Pf1N1B4_5546at 317.998 kb on + strand, within Pf1N1B4_5546at 317.998 kb on + strand, within Pf1N1B4_5546at 317.999 kb on - strand, within Pf1N1B4_5546at 317.999 kb on - strand, within Pf1N1B4_5546at 317.999 kb on - strand, within Pf1N1B4_5546at 318.105 kb on + strand, within Pf1N1B4_5546at 318.130 kb on + strand, within Pf1N1B4_5546at 318.131 kb on - strand, within Pf1N1B4_5546at 318.167 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.249 kb on + strand, within Pf1N1B4_5546at 318.250 kb on - strand, within Pf1N1B4_5546at 318.253 kb on + strandat 318.253 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.315 kb on + strand, within Pf1N1B4_5546at 318.316 kb on - strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.358 kb on + strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.359 kb on - strand, within Pf1N1B4_5546at 318.432 kb on + strandat 318.440 kb on + strandat 318.610 kb on - strand, within Pf1N1B4_5547at 318.651 kb on - strand, within Pf1N1B4_5547at 318.896 kb on + strandat 318.897 kb on - strandat 318.920 kb on + strandat 318.986 kb on - strandat 318.986 kb on - strandat 318.986 kb on - strandat 318.986 kb on - strandat 319.027 kb on + strandat 319.028 kb on - strandat 319.028 kb on - strandat 319.028 kb on - strandat 319.028 kb on - strandat 319.044 kb on + strand, within Pf1N1B4_5548at 319.060 kb on - strand, within Pf1N1B4_5548at 319.060 kb on - strand, within Pf1N1B4_5548at 319.060 kb on - strand, within Pf1N1B4_5548at 319.182 kb on + strand, within Pf1N1B4_5548at 319.360 kb on - strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548at 319.408 kb on + strand, within Pf1N1B4_5548at 319.502 kb on + strand, within Pf1N1B4_5548at 319.503 kb on - strand, within Pf1N1B4_5548at 319.503 kb on - strand, within Pf1N1B4_5548at 319.596 kb on + strand, within Pf1N1B4_5548at 319.611 kb on + strand, within Pf1N1B4_5548at 319.611 kb on + strand, within Pf1N1B4_5548at 319.612 kb on - strand, within Pf1N1B4_5548at 319.627 kb on + strand, within Pf1N1B4_5548at 319.627 kb on + strand, within Pf1N1B4_5548at 319.627 kb on + strand, within Pf1N1B4_5548at 319.627 kb on + strand, within Pf1N1B4_5548at 319.628 kb on - strand, within Pf1N1B4_5548at 319.716 kb on + strand, within Pf1N1B4_5548at 319.730 kb on - strand, within Pf1N1B4_5548at 319.779 kb on + strand, within Pf1N1B4_5548at 319.779 kb on + strand, within Pf1N1B4_5548at 319.779 kb on + strand, within Pf1N1B4_5548at 319.779 kb on + strand, within Pf1N1B4_5548

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml
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317,518 - -0.1
317,532 + -0.1
317,533 - +0.2
317,533 - +0.4
317,533 - +1.9
317,823 + Pf1N1B4_5546 0.32 +1.0
317,878 + Pf1N1B4_5546 0.38 -0.0
317,892 + Pf1N1B4_5546 0.40 +0.4
317,892 + Pf1N1B4_5546 0.40 +0.2
317,892 + Pf1N1B4_5546 0.40 -0.5
317,893 - Pf1N1B4_5546 0.40 +1.7
317,940 + Pf1N1B4_5546 0.45 +0.5
317,948 + Pf1N1B4_5546 0.46 +0.4
317,948 + Pf1N1B4_5546 0.46 -0.1
317,985 + Pf1N1B4_5546 0.49 +1.9
317,998 + Pf1N1B4_5546 0.51 -0.1
317,998 + Pf1N1B4_5546 0.51 +0.8
317,999 - Pf1N1B4_5546 0.51 +0.7
317,999 - Pf1N1B4_5546 0.51 -0.6
317,999 - Pf1N1B4_5546 0.51 +0.5
318,105 + Pf1N1B4_5546 0.62 -0.4
318,130 + Pf1N1B4_5546 0.65 +1.6
318,131 - Pf1N1B4_5546 0.65 +0.6
318,167 + Pf1N1B4_5546 0.69 -0.0
318,249 + Pf1N1B4_5546 0.77 +0.1
318,249 + Pf1N1B4_5546 0.77 -2.0
318,249 + Pf1N1B4_5546 0.77 -0.1
318,249 + Pf1N1B4_5546 0.77 +0.2
318,250 - Pf1N1B4_5546 0.78 +1.8
318,253 + +3.2
318,253 + Pf1N1B4_5546 0.78 +1.8
318,315 + Pf1N1B4_5546 0.85 +0.0
318,315 + Pf1N1B4_5546 0.85 -1.6
318,315 + Pf1N1B4_5546 0.85 +1.4
318,315 + Pf1N1B4_5546 0.85 -0.8
318,316 - Pf1N1B4_5546 0.85 -0.4
318,358 + Pf1N1B4_5546 0.89 -1.4
318,358 + Pf1N1B4_5546 0.89 -1.4
318,358 + Pf1N1B4_5546 0.89 -2.4
318,358 + Pf1N1B4_5546 0.89 +0.6
318,358 + Pf1N1B4_5546 0.89 +1.8
318,359 - Pf1N1B4_5546 0.89 +0.8
318,359 - Pf1N1B4_5546 0.89 +0.2
318,359 - Pf1N1B4_5546 0.89 +0.2
318,359 - Pf1N1B4_5546 0.89 +1.7
318,432 + +0.4
318,440 + +1.6
318,610 - Pf1N1B4_5547 0.40 +1.2
318,651 - Pf1N1B4_5547 0.54 -0.1
318,896 + +1.2
318,897 - +0.2
318,920 + +0.9
318,986 - -1.8
318,986 - +1.2
318,986 - +0.4
318,986 - +0.8
319,027 + +1.2
319,028 - -2.0
319,028 - +0.7
319,028 - -1.8
319,028 - -2.1
319,044 + Pf1N1B4_5548 0.11 -2.0
319,060 - Pf1N1B4_5548 0.13 +0.4
319,060 - Pf1N1B4_5548 0.13 -0.4
319,060 - Pf1N1B4_5548 0.13 -1.7
319,182 + Pf1N1B4_5548 0.25 -0.6
319,360 - Pf1N1B4_5548 0.42 +1.2
319,408 + Pf1N1B4_5548 0.47 -0.0
319,408 + Pf1N1B4_5548 0.47 +0.8
319,408 + Pf1N1B4_5548 0.47 +0.2
319,408 + Pf1N1B4_5548 0.47 -0.4
319,502 + Pf1N1B4_5548 0.56 +1.2
319,503 - Pf1N1B4_5548 0.57 +0.8
319,503 - Pf1N1B4_5548 0.57 +1.9
319,596 + Pf1N1B4_5548 0.66 -0.4
319,611 + Pf1N1B4_5548 0.67 -0.4
319,611 + Pf1N1B4_5548 0.67 +0.2
319,612 - Pf1N1B4_5548 0.67 +1.4
319,627 + Pf1N1B4_5548 0.69 +0.2
319,627 + Pf1N1B4_5548 0.69 +1.2
319,627 + Pf1N1B4_5548 0.69 +0.7
319,627 + Pf1N1B4_5548 0.69 +1.0
319,628 - Pf1N1B4_5548 0.69 -2.1
319,716 + Pf1N1B4_5548 0.78 +1.8
319,730 - Pf1N1B4_5548 0.79 +0.6
319,779 + Pf1N1B4_5548 0.84 +0.4
319,779 + Pf1N1B4_5548 0.84 +2.0
319,779 + Pf1N1B4_5548 0.84 -0.0
319,779 + Pf1N1B4_5548 0.84 +1.6

Or see this region's nucleotide sequence