Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5533 and Pf1N1B4_5534 are separated by 395 nucleotides
Pf1N1B4_5533 - cobS protein, putative, at 295,253 to 296,158
_5533
Pf1N1B4_5534 - hypothetical protein, at 296,554 to 296,670
_5534
Position (kb) 295
296
297 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 294.445 kb on + strand at 294.448 kb on + strand at 294.448 kb on + strand at 294.448 kb on + strand at 294.448 kb on + strand at 294.476 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.479 kb on + strand at 294.514 kb on + strand at 294.575 kb on + strand at 294.609 kb on + strand at 294.609 kb on + strand at 294.610 kb on - strand at 294.736 kb on - strand at 294.809 kb on + strand at 294.809 kb on + strand at 294.810 kb on - strand at 294.834 kb on - strand at 294.869 kb on + strand at 294.989 kb on + strand at 294.995 kb on + strand at 294.995 kb on + strand at 294.995 kb on + strand at 294.995 kb on + strand at 294.996 kb on - strand at 294.996 kb on - strand at 294.996 kb on - strand at 295.020 kb on - strand at 295.192 kb on + strand at 295.205 kb on - strand at 295.205 kb on - strand at 295.229 kb on - strand at 295.229 kb on - strand at 295.479 kb on - strand, within Pf1N1B4_5533 at 295.660 kb on + strand, within Pf1N1B4_5533 at 295.661 kb on - strand, within Pf1N1B4_5533 at 295.819 kb on + strand, within Pf1N1B4_5533 at 295.946 kb on - strand, within Pf1N1B4_5533 at 295.962 kb on + strand, within Pf1N1B4_5533 at 295.962 kb on + strand, within Pf1N1B4_5533 at 295.963 kb on - strand, within Pf1N1B4_5533 at 296.057 kb on - strand, within Pf1N1B4_5533 at 296.199 kb on + strand at 296.199 kb on + strand at 296.199 kb on + strand at 296.199 kb on + strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.200 kb on - strand at 296.291 kb on + strand at 296.304 kb on + strand at 296.304 kb on + strand at 296.334 kb on + strand at 296.552 kb on - strand at 296.692 kb on + strand at 296.693 kb on - strand at 296.745 kb on + strand at 296.745 kb on + strand at 296.745 kb on + strand at 296.904 kb on - strand at 296.914 kb on + strand at 296.932 kb on + strand at 296.932 kb on + strand at 297.054 kb on - strand at 297.088 kb on + strand at 297.110 kb on + strand at 297.111 kb on - strand at 297.114 kb on + strand at 297.115 kb on - strand at 297.146 kb on - strand at 297.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 294,445 + +0.5 294,448 + -0.8 294,448 + -0.7 294,448 + -0.2 294,448 + +0.9 294,476 + +0.6 294,479 + -0.4 294,479 + +2.2 294,479 + -0.3 294,479 + +0.9 294,479 + -1.1 294,479 + +1.1 294,514 + -0.4 294,575 + +1.9 294,609 + +1.2 294,609 + -0.7 294,610 - -1.8 294,736 - +0.6 294,809 + -0.8 294,809 + -0.5 294,810 - +1.0 294,834 - -0.4 294,869 + +0.8 294,989 + +0.2 294,995 + -1.4 294,995 + +0.4 294,995 + +1.0 294,995 + +1.7 294,996 - +0.1 294,996 - +1.3 294,996 - -0.4 295,020 - -0.2 295,192 + +0.2 295,205 - +0.2 295,205 - +0.5 295,229 - -0.1 295,229 - +0.7 295,479 - Pf1N1B4_5533 0.25 -0.9 295,660 + Pf1N1B4_5533 0.45 +0.9 295,661 - Pf1N1B4_5533 0.45 -1.4 295,819 + Pf1N1B4_5533 0.62 -1.1 295,946 - Pf1N1B4_5533 0.76 +1.0 295,962 + Pf1N1B4_5533 0.78 -0.4 295,962 + Pf1N1B4_5533 0.78 -1.1 295,963 - Pf1N1B4_5533 0.78 -0.8 296,057 - Pf1N1B4_5533 0.89 -0.9 296,199 + +1.6 296,199 + +1.6 296,199 + +0.2 296,199 + -0.0 296,200 - -1.4 296,200 - -0.4 296,200 - -0.5 296,200 - +0.2 296,200 - +1.6 296,200 - -1.8 296,200 - +0.9 296,291 + -0.9 296,304 + +1.5 296,304 + +3.2 296,334 + +1.9 296,552 - +0.5 296,692 + -0.4 296,693 - -0.8 296,745 + -0.1 296,745 + +0.2 296,745 + +1.2 296,904 - -1.4 296,914 + +1.9 296,932 + -0.8 296,932 + +1.6 297,054 - +0.3 297,088 + -1.1 297,110 + -0.4 297,111 - -1.1 297,114 + +1.8 297,115 - +0.4 297,146 - -1.6 297,148 + +0.4
Or see this region's nucleotide sequence