Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5434 and Pf1N1B4_5435 are separated by 704 nucleotides Pf1N1B4_5435 and Pf1N1B4_5436 are separated by 700 nucleotides
Pf1N1B4_5434 - HIPA PROTEIN, at 173,694 to 175,007
_5434
Pf1N1B4_5435 - hypothetical protein, at 175,712 to 175,990
_5435
Pf1N1B4_5436 - Probable helicase, at 176,691 to 178,637
_5436
Position (kb)
175
176 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 174.730 kb on + strand, within Pf1N1B4_5434 at 174.731 kb on - strand, within Pf1N1B4_5434 at 174.744 kb on + strand, within Pf1N1B4_5434 at 174.762 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.805 kb on + strand, within Pf1N1B4_5434 at 174.827 kb on + strand, within Pf1N1B4_5434 at 174.911 kb on + strand at 174.911 kb on + strand at 174.911 kb on + strand at 174.911 kb on + strand at 174.911 kb on + strand at 174.912 kb on - strand at 174.927 kb on + strand at 175.005 kb on + strand at 175.005 kb on + strand at 175.005 kb on + strand at 175.005 kb on + strand at 175.050 kb on + strand at 175.114 kb on + strand at 175.115 kb on - strand at 175.148 kb on + strand at 175.157 kb on + strand at 175.157 kb on + strand at 175.157 kb on + strand at 175.158 kb on - strand at 175.158 kb on - strand at 175.173 kb on + strand at 175.181 kb on + strand at 175.182 kb on - strand at 175.312 kb on + strand at 175.354 kb on + strand at 175.407 kb on + strand at 175.407 kb on + strand at 175.408 kb on - strand at 175.431 kb on - strand at 175.613 kb on + strand at 175.614 kb on - strand at 175.691 kb on + strand at 175.751 kb on + strand, within Pf1N1B4_5435 at 175.751 kb on + strand, within Pf1N1B4_5435 at 175.968 kb on + strand at 176.196 kb on + strand at 176.284 kb on + strand at 176.402 kb on + strand at 176.402 kb on + strand at 176.403 kb on - strand at 176.438 kb on + strand at 176.438 kb on + strand at 176.438 kb on + strand at 176.533 kb on + strand at 176.592 kb on + strand at 176.592 kb on + strand at 176.592 kb on + strand at 176.593 kb on - strand at 176.593 kb on - strand at 176.593 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 174,730 + Pf1N1B4_5434 0.79 +1.5 174,731 - Pf1N1B4_5434 0.79 +0.4 174,744 + Pf1N1B4_5434 0.80 +2.8 174,762 + Pf1N1B4_5434 0.81 +1.0 174,805 + Pf1N1B4_5434 0.85 +1.3 174,805 + Pf1N1B4_5434 0.85 +0.9 174,805 + Pf1N1B4_5434 0.85 -2.9 174,805 + Pf1N1B4_5434 0.85 +0.9 174,805 + Pf1N1B4_5434 0.85 -1.4 174,827 + Pf1N1B4_5434 0.86 -0.1 174,911 + -0.1 174,911 + +0.6 174,911 + +1.5 174,911 + +0.2 174,911 + +0.8 174,912 - +0.7 174,927 + -0.4 175,005 + +0.9 175,005 + -1.8 175,005 + +1.2 175,005 + +1.8 175,050 + +0.8 175,114 + -0.3 175,115 - -0.7 175,148 + +1.0 175,157 + -0.8 175,157 + +0.4 175,157 + +0.3 175,158 - +2.2 175,158 - -0.2 175,173 + -0.6 175,181 + -1.4 175,182 - +0.4 175,312 + +0.7 175,354 + -0.0 175,407 + -0.0 175,407 + +0.5 175,408 - +0.9 175,431 - -1.1 175,613 + -2.9 175,614 - -1.4 175,691 + +1.6 175,751 + Pf1N1B4_5435 0.14 -2.1 175,751 + Pf1N1B4_5435 0.14 -0.3 175,968 + -0.1 176,196 + +1.4 176,284 + +1.0 176,402 + +0.2 176,402 + -0.4 176,403 - +1.0 176,438 + -1.0 176,438 + +0.9 176,438 + -0.6 176,533 + -2.0 176,592 + +1.2 176,592 + -1.4 176,592 + -0.1 176,593 - +1.3 176,593 - +1.9 176,593 - -0.6
Or see this region's nucleotide sequence