Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5419 and Pf1N1B4_5420 are separated by 264 nucleotides Pf1N1B4_5420 and Pf1N1B4_5421 are separated by 112 nucleotides Pf1N1B4_5421 and Pf1N1B4_5422 are separated by 121 nucleotides
Pf1N1B4_5419 - hypothetical protein, at 159,566 to 159,685
_5419
Pf1N1B4_5420 - hypothetical protein, at 159,950 to 160,297
_5420
Pf1N1B4_5421 - hypothetical protein, at 160,410 to 160,661
_5421
Pf1N1B4_5422 - hypothetical protein, at 160,783 to 162,591
_5422
Position (kb)
159
160
161 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 158.982 kb on + strand at 159.039 kb on + strand at 159.046 kb on - strand at 159.136 kb on + strand at 159.165 kb on - strand at 159.359 kb on + strand at 159.465 kb on + strand at 159.497 kb on + strand at 159.497 kb on + strand at 159.497 kb on + strand at 159.497 kb on + strand at 159.567 kb on + strand at 159.685 kb on + strand at 159.685 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.785 kb on + strand at 159.786 kb on - strand at 159.786 kb on - strand at 159.786 kb on - strand at 159.786 kb on - strand at 159.877 kb on + strand at 159.877 kb on + strand at 159.978 kb on + strand at 159.978 kb on + strand at 159.978 kb on + strand at 160.191 kb on + strand, within Pf1N1B4_5420 at 160.192 kb on - strand, within Pf1N1B4_5420 at 160.218 kb on + strand, within Pf1N1B4_5420 at 160.218 kb on + strand, within Pf1N1B4_5420 at 160.219 kb on - strand, within Pf1N1B4_5420 at 160.219 kb on - strand, within Pf1N1B4_5420 at 160.314 kb on + strand at 160.315 kb on - strand at 160.317 kb on + strand at 160.317 kb on + strand at 160.317 kb on + strand at 160.318 kb on - strand at 160.352 kb on - strand at 160.352 kb on - strand at 160.370 kb on - strand at 160.373 kb on + strand at 160.373 kb on + strand at 160.373 kb on + strand at 160.532 kb on - strand, within Pf1N1B4_5421 at 160.636 kb on + strand at 160.636 kb on + strand at 160.636 kb on + strand at 160.636 kb on + strand at 160.637 kb on - strand at 160.693 kb on + strand at 160.694 kb on - strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.695 kb on + strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.696 kb on - strand at 160.747 kb on + strand at 160.747 kb on + strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.748 kb on - strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.749 kb on + strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.750 kb on - strand at 160.774 kb on + strand at 160.774 kb on + strand at 160.804 kb on + strand at 160.823 kb on + strand at 160.851 kb on + strand at 161.100 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.132 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.201 kb on + strand, within Pf1N1B4_5422 at 161.202 kb on - strand, within Pf1N1B4_5422 at 161.236 kb on + strand, within Pf1N1B4_5422 at 161.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 158,982 + -1.3 159,039 + -1.1 159,046 - -0.2 159,136 + -0.6 159,165 - -0.6 159,359 + +0.2 159,465 + -0.2 159,497 + -1.1 159,497 + -0.2 159,497 + +1.2 159,497 + -1.4 159,567 + +0.2 159,685 + -0.8 159,685 + -1.8 159,785 + +0.1 159,785 + +1.2 159,785 + +0.8 159,785 + -2.0 159,785 + -1.5 159,785 + -0.2 159,785 + -0.6 159,785 + +0.6 159,786 - -0.8 159,786 - -0.5 159,786 - -1.1 159,786 - +0.2 159,877 + -0.3 159,877 + +0.2 159,978 + -0.6 159,978 + +0.2 159,978 + -0.7 160,191 + Pf1N1B4_5420 0.69 -0.1 160,192 - Pf1N1B4_5420 0.70 -0.8 160,218 + Pf1N1B4_5420 0.77 -0.5 160,218 + Pf1N1B4_5420 0.77 -0.4 160,219 - Pf1N1B4_5420 0.77 -1.1 160,219 - Pf1N1B4_5420 0.77 -0.8 160,314 + +1.0 160,315 - +2.2 160,317 + +1.6 160,317 + +0.0 160,317 + +0.5 160,318 - -1.1 160,352 - +0.2 160,352 - +0.8 160,370 - +0.2 160,373 + -1.1 160,373 + +0.5 160,373 + +1.5 160,532 - Pf1N1B4_5421 0.48 +0.9 160,636 + +1.8 160,636 + +0.4 160,636 + +0.9 160,636 + +0.6 160,637 - +0.3 160,693 + -0.3 160,694 - -1.8 160,695 + -0.8 160,695 + +0.6 160,695 + -0.7 160,695 + +0.2 160,695 + +2.5 160,696 - +0.2 160,696 - -0.4 160,696 - -0.8 160,696 - -0.4 160,696 - +2.8 160,747 + +0.8 160,747 + -0.4 160,748 - +0.2 160,748 - +1.2 160,748 - -1.5 160,748 - +0.5 160,748 - +1.2 160,749 + -0.8 160,749 + -0.4 160,749 + -0.4 160,749 + -0.8 160,749 + +0.4 160,749 + +0.2 160,750 - +0.2 160,750 - -1.4 160,750 - -1.5 160,750 - +0.2 160,750 - -0.1 160,750 - +0.6 160,774 + -0.4 160,774 + +1.2 160,804 + +0.5 160,823 + +2.5 160,851 + +1.2 161,100 + Pf1N1B4_5422 0.18 -1.1 161,132 + Pf1N1B4_5422 0.19 +1.2 161,132 + Pf1N1B4_5422 0.19 +0.0 161,132 + Pf1N1B4_5422 0.19 +1.2 161,132 + Pf1N1B4_5422 0.19 +0.7 161,201 + Pf1N1B4_5422 0.23 +0.4 161,201 + +1.8 161,201 + Pf1N1B4_5422 0.23 +0.6 161,201 + Pf1N1B4_5422 0.23 -0.4 161,201 + Pf1N1B4_5422 0.23 -0.1 161,201 + Pf1N1B4_5422 0.23 +1.8 161,201 + Pf1N1B4_5422 0.23 -1.6 161,202 - Pf1N1B4_5422 0.23 -1.6 161,236 + Pf1N1B4_5422 0.25 -1.0 161,237 - -0.8
Or see this region's nucleotide sequence