Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5375 and Pf1N1B4_5376 are separated by 164 nucleotides Pf1N1B4_5376 and Pf1N1B4_5377 are separated by 206 nucleotides Pf1N1B4_5377 and Pf1N1B4_5378 are separated by 577 nucleotides
Pf1N1B4_5375 - putative DNA methyltransferase, at 107,874 to 109,142
_5375
Pf1N1B4_5376 - hypothetical protein, at 109,307 to 109,810
_5376
Pf1N1B4_5377 - Uncharacterized domain COG3236 / GTP cyclohydrolase II (EC 3.5.4.25), at 110,017 to 110,511
_5377
Pf1N1B4_5378 - Phage protein, at 111,089 to 112,282
_5378
Position (kb)
110
111 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 109.078 kb on + strand at 109.078 kb on + strand at 109.078 kb on + strand at 109.079 kb on - strand at 109.213 kb on + strand at 109.213 kb on + strand at 109.214 kb on - strand at 109.300 kb on - strand at 109.300 kb on - strand at 109.357 kb on + strand at 109.358 kb on - strand, within Pf1N1B4_5376 at 109.361 kb on - strand, within Pf1N1B4_5376 at 109.482 kb on + strand, within Pf1N1B4_5376 at 109.483 kb on - strand, within Pf1N1B4_5376 at 109.483 kb on - strand, within Pf1N1B4_5376 at 109.612 kb on + strand, within Pf1N1B4_5376 at 109.777 kb on + strand at 109.791 kb on + strand at 109.791 kb on + strand at 109.791 kb on + strand at 109.791 kb on + strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.792 kb on - strand at 109.966 kb on - strand at 109.966 kb on - strand at 109.966 kb on - strand at 109.966 kb on - strand at 110.005 kb on + strand at 110.005 kb on + strand at 110.005 kb on + strand at 110.019 kb on - strand at 110.154 kb on - strand, within Pf1N1B4_5377 at 110.170 kb on + strand, within Pf1N1B4_5377 at 110.263 kb on - strand, within Pf1N1B4_5377 at 110.289 kb on - strand, within Pf1N1B4_5377 at 110.390 kb on + strand, within Pf1N1B4_5377 at 110.391 kb on - strand, within Pf1N1B4_5377 at 110.494 kb on - strand at 110.494 kb on - strand at 110.494 kb on - strand at 110.580 kb on + strand at 110.581 kb on - strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.605 kb on + strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.606 kb on - strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.609 kb on + strand at 110.610 kb on - strand at 110.610 kb on - strand at 110.653 kb on + strand at 110.653 kb on + strand at 110.653 kb on + strand at 110.654 kb on - strand at 110.654 kb on - strand at 110.689 kb on + strand at 110.690 kb on - strand at 110.697 kb on + strand at 110.697 kb on + strand at 110.697 kb on + strand at 110.698 kb on - strand at 110.698 kb on - strand at 110.698 kb on - strand at 110.698 kb on - strand at 111.110 kb on + strand at 111.110 kb on + strand at 111.111 kb on - strand at 111.111 kb on - strand at 111.111 kb on - strand at 111.111 kb on - strand at 111.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 109,078 + +0.8 109,078 + +0.4 109,078 + -1.0 109,079 - +0.9 109,213 + +1.8 109,213 + +1.2 109,214 - +1.2 109,300 - +0.2 109,300 - +0.6 109,357 + -1.1 109,358 - Pf1N1B4_5376 0.10 -1.1 109,361 - Pf1N1B4_5376 0.11 +2.8 109,482 + Pf1N1B4_5376 0.35 +1.8 109,483 - Pf1N1B4_5376 0.35 -0.4 109,483 - Pf1N1B4_5376 0.35 +0.9 109,612 + Pf1N1B4_5376 0.61 +0.6 109,777 + +1.0 109,791 + +0.4 109,791 + -0.8 109,791 + +0.7 109,791 + +1.8 109,792 - +2.5 109,792 - -0.5 109,792 - +1.2 109,792 - -0.8 109,792 - -0.1 109,792 - -1.1 109,966 - +1.2 109,966 - +0.2 109,966 - -2.1 109,966 - -1.4 110,005 + +0.2 110,005 + +0.6 110,005 + +1.0 110,019 - +1.6 110,154 - Pf1N1B4_5377 0.28 +0.2 110,170 + Pf1N1B4_5377 0.31 +0.8 110,263 - Pf1N1B4_5377 0.50 +0.0 110,289 - Pf1N1B4_5377 0.55 +1.4 110,390 + Pf1N1B4_5377 0.75 +1.2 110,391 - Pf1N1B4_5377 0.76 +0.8 110,494 - +0.7 110,494 - -1.0 110,494 - -1.4 110,580 + +0.6 110,581 - +0.6 110,605 + +0.7 110,605 + +2.2 110,605 + +1.6 110,605 + -0.0 110,605 + -0.5 110,605 + +0.1 110,606 - -0.4 110,606 - -0.4 110,606 - -0.4 110,606 - +0.4 110,606 - +0.6 110,606 - +1.2 110,606 - -1.1 110,606 - +0.4 110,606 - +0.2 110,609 + +0.0 110,609 + -0.6 110,609 + +0.4 110,609 + +1.7 110,610 - -1.1 110,610 - +0.2 110,653 + -0.0 110,653 + -0.4 110,653 + -1.3 110,654 - +0.6 110,654 - +0.4 110,689 + -2.1 110,690 - +1.0 110,697 + -0.7 110,697 + -0.2 110,697 + -0.4 110,698 - +0.5 110,698 - -0.2 110,698 - +2.2 110,698 - +0.4 111,110 + +1.2 111,110 + +0.6 111,111 - +1.6 111,111 - -1.6 111,111 - -0.0 111,111 - +0.0 111,111 - +0.2
Or see this region's nucleotide sequence