Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5368

Experiment: Sisomicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5366 and Pf1N1B4_5367 are separated by 291 nucleotidesPf1N1B4_5367 and Pf1N1B4_5368 are separated by 16 nucleotidesPf1N1B4_5368 and Pf1N1B4_5369 are separated by 470 nucleotides Pf1N1B4_5366 - hypothetical protein, at 99,603 to 99,803 _5366 Pf1N1B4_5367 - hypothetical protein, at 100,095 to 100,583 _5367 Pf1N1B4_5368 - hypothetical protein, at 100,600 to 100,914 _5368 Pf1N1B4_5369 - hypothetical protein, at 101,385 to 101,894 _5369 Position (kb) 100 101Strain fitness (log2 ratio) -2 -1 0 1 2at 99.746 kb on + strand, within Pf1N1B4_5366at 99.895 kb on + strandat 99.896 kb on - strandat 99.896 kb on - strandat 99.896 kb on - strandat 99.896 kb on - strandat 100.062 kb on + strandat 100.077 kb on + strandat 100.077 kb on + strandat 100.077 kb on + strandat 100.174 kb on + strand, within Pf1N1B4_5367at 100.249 kb on + strand, within Pf1N1B4_5367at 100.273 kb on + strand, within Pf1N1B4_5367at 100.274 kb on - strand, within Pf1N1B4_5367at 100.274 kb on - strand, within Pf1N1B4_5367at 100.274 kb on - strand, within Pf1N1B4_5367at 100.313 kb on + strand, within Pf1N1B4_5367at 100.313 kb on + strand, within Pf1N1B4_5367at 100.313 kb on + strand, within Pf1N1B4_5367at 100.313 kb on + strand, within Pf1N1B4_5367at 100.313 kb on + strand, within Pf1N1B4_5367at 100.314 kb on - strand, within Pf1N1B4_5367at 100.468 kb on + strand, within Pf1N1B4_5367at 100.468 kb on + strand, within Pf1N1B4_5367at 100.534 kb on + strand, within Pf1N1B4_5367at 100.534 kb on + strand, within Pf1N1B4_5367at 100.534 kb on + strand, within Pf1N1B4_5367at 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.587 kb on + strandat 100.588 kb on - strandat 100.588 kb on - strandat 100.591 kb on + strandat 100.602 kb on - strandat 100.718 kb on + strand, within Pf1N1B4_5368at 100.718 kb on + strand, within Pf1N1B4_5368at 100.718 kb on + strand, within Pf1N1B4_5368at 100.719 kb on - strand, within Pf1N1B4_5368at 100.755 kb on - strand, within Pf1N1B4_5368at 100.788 kb on + strand, within Pf1N1B4_5368at 100.788 kb on + strand, within Pf1N1B4_5368at 100.789 kb on - strand, within Pf1N1B4_5368at 100.789 kb on - strand, within Pf1N1B4_5368at 100.790 kb on + strand, within Pf1N1B4_5368at 100.791 kb on - strand, within Pf1N1B4_5368at 100.832 kb on + strand, within Pf1N1B4_5368at 100.832 kb on + strand, within Pf1N1B4_5368at 100.832 kb on + strandat 100.832 kb on + strand, within Pf1N1B4_5368at 100.832 kb on + strand, within Pf1N1B4_5368at 100.832 kb on + strand, within Pf1N1B4_5368at 100.833 kb on - strand, within Pf1N1B4_5368at 100.833 kb on - strand, within Pf1N1B4_5368at 100.916 kb on + strandat 100.916 kb on + strandat 100.916 kb on + strandat 100.916 kb on + strandat 100.917 kb on - strandat 100.917 kb on - strandat 100.917 kb on - strandat 101.118 kb on + strandat 101.118 kb on + strandat 101.118 kb on + strandat 101.149 kb on + strandat 101.149 kb on + strandat 101.149 kb on + strandat 101.149 kb on + strandat 101.149 kb on + strandat 101.149 kb on + strandat 101.149 kb on + strandat 101.149 kb on + strandat 101.152 kb on - strandat 101.152 kb on - strandat 101.437 kb on + strand, within Pf1N1B4_5369at 101.503 kb on + strand, within Pf1N1B4_5369at 101.504 kb on - strand, within Pf1N1B4_5369at 101.504 kb on - strand, within Pf1N1B4_5369at 101.504 kb on - strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strandat 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.506 kb on + strand, within Pf1N1B4_5369at 101.507 kb on - strand, within Pf1N1B4_5369at 101.507 kb on - strand, within Pf1N1B4_5369at 101.507 kb on - strand, within Pf1N1B4_5369at 101.508 kb on + strand, within Pf1N1B4_5369at 101.509 kb on - strandat 101.509 kb on - strand, within Pf1N1B4_5369at 101.509 kb on - strand, within Pf1N1B4_5369at 101.542 kb on + strand, within Pf1N1B4_5369at 101.542 kb on + strandat 101.542 kb on + strand, within Pf1N1B4_5369at 101.543 kb on - strand, within Pf1N1B4_5369at 101.543 kb on - strand, within Pf1N1B4_5369at 101.595 kb on + strand, within Pf1N1B4_5369at 101.595 kb on + strand, within Pf1N1B4_5369at 101.595 kb on + strand, within Pf1N1B4_5369at 101.595 kb on + strand, within Pf1N1B4_5369at 101.596 kb on - strand, within Pf1N1B4_5369at 101.596 kb on - strand, within Pf1N1B4_5369at 101.596 kb on - strand, within Pf1N1B4_5369at 101.596 kb on - strand, within Pf1N1B4_5369at 101.598 kb on + strand, within Pf1N1B4_5369at 101.598 kb on + strand, within Pf1N1B4_5369at 101.598 kb on + strand, within Pf1N1B4_5369at 101.599 kb on - strand, within Pf1N1B4_5369at 101.802 kb on + strand, within Pf1N1B4_5369at 101.802 kb on + strand, within Pf1N1B4_5369at 101.803 kb on - strand, within Pf1N1B4_5369at 101.803 kb on - strand, within Pf1N1B4_5369at 101.803 kb on - strand, within Pf1N1B4_5369at 101.803 kb on - strand, within Pf1N1B4_5369at 101.803 kb on - strand, within Pf1N1B4_5369at 101.803 kb on - strand, within Pf1N1B4_5369at 101.803 kb on - strand, within Pf1N1B4_5369at 101.857 kb on + strandat 101.857 kb on + strandat 101.858 kb on - strandat 101.858 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml
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99,746 + Pf1N1B4_5366 0.71 +0.7
99,895 + +0.8
99,896 - -1.1
99,896 - -0.6
99,896 - -0.4
99,896 - +2.2
100,062 + -0.2
100,077 + -0.8
100,077 + +0.9
100,077 + +0.2
100,174 + Pf1N1B4_5367 0.16 -0.8
100,249 + Pf1N1B4_5367 0.31 +0.4
100,273 + Pf1N1B4_5367 0.36 -0.6
100,274 - Pf1N1B4_5367 0.37 +0.2
100,274 - Pf1N1B4_5367 0.37 +0.6
100,274 - Pf1N1B4_5367 0.37 -0.5
100,313 + Pf1N1B4_5367 0.45 +0.8
100,313 + Pf1N1B4_5367 0.45 +0.6
100,313 + Pf1N1B4_5367 0.45 +0.8
100,313 + Pf1N1B4_5367 0.45 -0.2
100,313 + Pf1N1B4_5367 0.45 +0.6
100,314 - Pf1N1B4_5367 0.45 -0.4
100,468 + Pf1N1B4_5367 0.76 +0.6
100,468 + Pf1N1B4_5367 0.76 +1.8
100,534 + Pf1N1B4_5367 0.90 +0.2
100,534 + Pf1N1B4_5367 0.90 -0.4
100,534 + Pf1N1B4_5367 0.90 -0.1
100,587 + +0.6
100,587 + +0.4
100,587 + +0.2
100,587 + +0.2
100,587 + +0.3
100,587 + -0.8
100,587 + -0.5
100,587 + +2.2
100,587 + +0.8
100,587 + -0.2
100,588 - -0.1
100,588 - -0.8
100,591 + +1.3
100,602 - -1.0
100,718 + Pf1N1B4_5368 0.37 -0.6
100,718 + Pf1N1B4_5368 0.37 -1.5
100,718 + Pf1N1B4_5368 0.37 -1.0
100,719 - Pf1N1B4_5368 0.38 -1.4
100,755 - Pf1N1B4_5368 0.49 -0.4
100,788 + Pf1N1B4_5368 0.60 +0.1
100,788 + Pf1N1B4_5368 0.60 -1.4
100,789 - Pf1N1B4_5368 0.60 -1.8
100,789 - Pf1N1B4_5368 0.60 +0.8
100,790 + Pf1N1B4_5368 0.60 +0.7
100,791 - Pf1N1B4_5368 0.61 +0.6
100,832 + Pf1N1B4_5368 0.74 -1.1
100,832 + Pf1N1B4_5368 0.74 -1.4
100,832 + +1.2
100,832 + Pf1N1B4_5368 0.74 -1.8
100,832 + Pf1N1B4_5368 0.74 +0.4
100,832 + Pf1N1B4_5368 0.74 -1.9
100,833 - Pf1N1B4_5368 0.74 -1.8
100,833 - Pf1N1B4_5368 0.74 -1.6
100,916 + -0.6
100,916 + -0.1
100,916 + +0.1
100,916 + +2.2
100,917 - +0.2
100,917 - -2.0
100,917 - -1.4
101,118 + +0.6
101,118 + -0.6
101,118 + +1.8
101,149 + +0.3
101,149 + +1.5
101,149 + +1.2
101,149 + -1.8
101,149 + +0.6
101,149 + +1.0
101,149 + -0.4
101,149 + +0.6
101,152 - +0.0
101,152 - -0.2
101,437 + Pf1N1B4_5369 0.10 +0.0
101,503 + Pf1N1B4_5369 0.23 +0.7
101,504 - Pf1N1B4_5369 0.23 -0.4
101,504 - Pf1N1B4_5369 0.23 -0.4
101,504 - Pf1N1B4_5369 0.23 +0.1
101,506 + Pf1N1B4_5369 0.24 -0.5
101,506 + Pf1N1B4_5369 0.24 +1.8
101,506 + Pf1N1B4_5369 0.24 -0.0
101,506 + +0.2
101,506 + Pf1N1B4_5369 0.24 -1.0
101,506 + Pf1N1B4_5369 0.24 +0.2
101,506 + Pf1N1B4_5369 0.24 -2.1
101,506 + Pf1N1B4_5369 0.24 +0.2
101,506 + Pf1N1B4_5369 0.24 -2.0
101,506 + Pf1N1B4_5369 0.24 +0.0
101,507 - Pf1N1B4_5369 0.24 +0.7
101,507 - Pf1N1B4_5369 0.24 -0.4
101,507 - Pf1N1B4_5369 0.24 +1.9
101,508 + Pf1N1B4_5369 0.24 -0.4
101,509 - +1.2
101,509 - Pf1N1B4_5369 0.24 -1.2
101,509 - Pf1N1B4_5369 0.24 -0.8
101,542 + Pf1N1B4_5369 0.31 -0.4
101,542 + +0.2
101,542 + Pf1N1B4_5369 0.31 +1.6
101,543 - Pf1N1B4_5369 0.31 +0.5
101,543 - Pf1N1B4_5369 0.31 +0.2
101,595 + Pf1N1B4_5369 0.41 -1.1
101,595 + Pf1N1B4_5369 0.41 -2.0
101,595 + Pf1N1B4_5369 0.41 -1.4
101,595 + Pf1N1B4_5369 0.41 +0.2
101,596 - Pf1N1B4_5369 0.41 -0.1
101,596 - Pf1N1B4_5369 0.41 -0.3
101,596 - Pf1N1B4_5369 0.41 -0.4
101,596 - Pf1N1B4_5369 0.41 +0.6
101,598 + Pf1N1B4_5369 0.42 -0.7
101,598 + Pf1N1B4_5369 0.42 -0.6
101,598 + Pf1N1B4_5369 0.42 -0.0
101,599 - Pf1N1B4_5369 0.42 +0.4
101,802 + Pf1N1B4_5369 0.82 -0.8
101,802 + Pf1N1B4_5369 0.82 +0.2
101,803 - Pf1N1B4_5369 0.82 -0.1
101,803 - Pf1N1B4_5369 0.82 -1.3
101,803 - Pf1N1B4_5369 0.82 +0.4
101,803 - Pf1N1B4_5369 0.82 -1.3
101,803 - Pf1N1B4_5369 0.82 -0.2
101,803 - Pf1N1B4_5369 0.82 +0.1
101,803 - Pf1N1B4_5369 0.82 -0.4
101,857 + -0.4
101,857 + -0.6
101,858 - +0.6
101,858 - -0.3

Or see this region's nucleotide sequence