Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5101 and Pf1N1B4_5102 are separated by 3 nucleotides Pf1N1B4_5102 and Pf1N1B4_5103 overlap by 4 nucleotides Pf1N1B4_5103 and Pf1N1B4_5104 are separated by 8 nucleotides
Pf1N1B4_5101 - Proline iminopeptidase (EC 3.4.11.5), at 5,654,870 to 5,655,757
_5101
Pf1N1B4_5102 - Putative glutathione transporter, permease component, at 5,655,761 to 5,656,708
_5102
Pf1N1B4_5103 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 5,656,705 to 5,657,559
_5103
Pf1N1B4_5104 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 5,657,568 to 5,658,542
_5104
Position (kb) 5655
5656
5657 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5654.786 kb on - strand at 5654.786 kb on - strand at 5654.938 kb on + strand at 5654.938 kb on + strand at 5655.002 kb on + strand, within Pf1N1B4_5101 at 5655.002 kb on + strand at 5655.225 kb on - strand, within Pf1N1B4_5101 at 5655.696 kb on - strand at 5655.714 kb on - strand at 5655.727 kb on - strand at 5655.966 kb on - strand, within Pf1N1B4_5102 at 5655.971 kb on + strand at 5655.971 kb on + strand, within Pf1N1B4_5102 at 5656.242 kb on + strand, within Pf1N1B4_5102 at 5656.242 kb on + strand, within Pf1N1B4_5102 at 5656.455 kb on + strand, within Pf1N1B4_5102 at 5656.456 kb on - strand, within Pf1N1B4_5102 at 5656.569 kb on + strand, within Pf1N1B4_5102 at 5656.598 kb on + strand, within Pf1N1B4_5102 at 5656.598 kb on + strand, within Pf1N1B4_5102 at 5656.599 kb on - strand, within Pf1N1B4_5102 at 5656.599 kb on - strand, within Pf1N1B4_5102 at 5656.601 kb on - strand, within Pf1N1B4_5102 at 5656.869 kb on + strand, within Pf1N1B4_5103 at 5657.188 kb on + strand, within Pf1N1B4_5103 at 5657.378 kb on - strand, within Pf1N1B4_5103
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 5,654,786 - +0.2 5,654,786 - -0.9 5,654,938 + +0.9 5,654,938 + +0.0 5,655,002 + Pf1N1B4_5101 0.15 +4.0 5,655,002 + +1.4 5,655,225 - Pf1N1B4_5101 0.40 -0.1 5,655,696 - +2.5 5,655,714 - -1.0 5,655,727 - +0.9 5,655,966 - Pf1N1B4_5102 0.22 +1.4 5,655,971 + +1.9 5,655,971 + Pf1N1B4_5102 0.22 -1.1 5,656,242 + Pf1N1B4_5102 0.51 +1.6 5,656,242 + Pf1N1B4_5102 0.51 -1.4 5,656,455 + Pf1N1B4_5102 0.73 -1.6 5,656,456 - Pf1N1B4_5102 0.73 +0.6 5,656,569 + Pf1N1B4_5102 0.85 -0.9 5,656,598 + Pf1N1B4_5102 0.88 -0.6 5,656,598 + Pf1N1B4_5102 0.88 -1.8 5,656,599 - Pf1N1B4_5102 0.88 -0.6 5,656,599 - Pf1N1B4_5102 0.88 -0.9 5,656,601 - Pf1N1B4_5102 0.89 -0.4 5,656,869 + Pf1N1B4_5103 0.19 -1.1 5,657,188 + Pf1N1B4_5103 0.56 -0.8 5,657,378 - Pf1N1B4_5103 0.79 +1.0
Or see this region's nucleotide sequence