Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4890 and Pf1N1B4_4891 are separated by 122 nucleotides Pf1N1B4_4891 and Pf1N1B4_4892 are separated by 277 nucleotides Pf1N1B4_4892 and Pf1N1B4_4893 are separated by 51 nucleotides
Pf1N1B4_4890 - Cystathionine gamma-lyase (EC 4.4.1.1), at 5,412,449 to 5,413,630
_4890
Pf1N1B4_4891 - FIG00959288: hypothetical protein, at 5,413,753 to 5,414,028
_4891
Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121
_4892
Pf1N1B4_4893 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 5,415,173 to 5,416,579
_4893
Position (kb)
5414
5415
5416 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 5413.761 kb on - strand at 5414.306 kb on - strand at 5414.432 kb on + strand at 5414.432 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.540 kb on - strand, within Pf1N1B4_4892 at 5414.810 kb on + strand, within Pf1N1B4_4892 at 5414.880 kb on - strand, within Pf1N1B4_4892 at 5414.913 kb on - strand, within Pf1N1B4_4892 at 5414.919 kb on - strand, within Pf1N1B4_4892 at 5415.033 kb on - strand, within Pf1N1B4_4892 at 5415.308 kb on + strand at 5415.308 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.311 kb on + strand at 5415.710 kb on - strand, within Pf1N1B4_4893 at 5415.713 kb on + strand at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.713 kb on + strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.714 kb on - strand, within Pf1N1B4_4893 at 5415.797 kb on + strand at 5416.040 kb on + strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand, within Pf1N1B4_4893 at 5416.041 kb on - strand at 5416.071 kb on + strand, within Pf1N1B4_4893
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 5,413,761 - -0.0 5,414,306 - +0.1 5,414,432 + +0.9 5,414,432 + Pf1N1B4_4892 0.15 +1.9 5,414,483 + Pf1N1B4_4892 0.22 +1.3 5,414,483 + Pf1N1B4_4892 0.22 +0.8 5,414,540 - Pf1N1B4_4892 0.29 +0.3 5,414,810 + Pf1N1B4_4892 0.62 -0.1 5,414,880 - Pf1N1B4_4892 0.70 +1.0 5,414,913 - Pf1N1B4_4892 0.74 -1.7 5,414,919 - Pf1N1B4_4892 0.75 +0.5 5,415,033 - Pf1N1B4_4892 0.89 +2.5 5,415,308 + +1.3 5,415,308 + -0.9 5,415,311 + -0.7 5,415,311 + -1.6 5,415,311 + +0.3 5,415,710 - Pf1N1B4_4893 0.38 +0.9 5,415,713 + -0.3 5,415,713 + Pf1N1B4_4893 0.38 -1.5 5,415,713 + Pf1N1B4_4893 0.38 -1.3 5,415,713 + Pf1N1B4_4893 0.38 -0.2 5,415,714 - Pf1N1B4_4893 0.38 +0.8 5,415,714 - Pf1N1B4_4893 0.38 +1.9 5,415,797 + +0.3 5,416,040 + Pf1N1B4_4893 0.62 -0.7 5,416,041 - -0.7 5,416,041 - Pf1N1B4_4893 0.62 +3.9 5,416,041 - Pf1N1B4_4893 0.62 +1.9 5,416,041 - -0.7 5,416,071 + Pf1N1B4_4893 0.64 -0.1
Or see this region's nucleotide sequence